Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy: integrative diagnosis and bionomics

Luca, Francesca De; Vovlas, N.; Lucarelli, Giuseppe; Troccoli, Alberto; Radicci, V.; Fanelli, Elena; Cantalapiedra‐Navarrete, Carolina; Palomares-Rius, Juan E.; Castillo, Pablo

doi:10.1007/s10658-013-0212-9

Cited by 22 publications

(9 citation statements)

References 22 publications

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“…28S: Amplification of the 28S large ribosomal subunit (LSU) D2-D3 expansion segment included the primers D2A [5′-ACAAGTACCGTGAGGGAAAGTT-3′] and D3B [5′-TCGGAAGGAACCAGCTACTA-3′] and were amplified as previously described ( De Ley et al, 2005 ; Ye et al, 2007 ).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Skantar

Handoo

Kantor

et al. 2020

Journal of Nematology

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In the summer of 2016, a field of corn (Zea mays) in Spencer County, Indiana was observed with heavily stunted plants, and from the affected roots a large number of cysts were recovered. Soil samples were submitted to one of us (JF), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and the recovered second-stage juveniles (J2) that were examined morphologically conformed to the measurements of Vittatidera zeaphila, the goose cyst nematode originally described from Tennessee, USA in 2010. The molecular analysis of J2 showed the sample from Spencer County matched exactly with V. zeaphila according to ribosomal DNA markers ITS, 28S, and 18S, and with mitochondrial cytochrome oxidase I (COI). The nuclear marker heat shock protein 90 (Hsp90) was also analyzed for the first time from the Indiana population of V. zeaphila. Similarities to existing cyst nematode sequences are reported herein. Geographically, although the county is across the Ohio River from Kentucky, the previously reported Hickman County, Kentucky location and Indiana detection are approximately 200 miles apart. To the best of our knowledge, this is the first report of V. zeaphila in Indiana.

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Section: Methodsmentioning

confidence: 99%

“…and Heterodera spp. (DeLuca et al, 2013). The 28S tree from Bernard et al (2010) contained a wider range of heteroderid genera for comparison, with V. zeaphila as a sister taxon to Heterodera, Cactodera, Punctodera, Globodera, and Dolichodera.…”

Section: Journal Of Nematologymentioning

confidence: 99%

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Skantar

Handoo

Kantor

et al. 2020

Journal of Nematology

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“…Some of these characteristics can be used to distinguish our species from H. elachista that is described as smaller cysts with a fragile and thinner underbridge tending to be absent in old cysts (Nobbs et al, 1992;Subbotin et al, 2010). Heterodera elachista and Heterodera oryzae belong to 'Cyperi' group whose J2s have three incisors in the lateral field, vulva cones have ambifenestrate fenestration type, weak or absent underbridge with bullae present or absent (Subbotin et al, 2010;De Luca et al, 2013). According to Nobbs et al (1992), H. elachista second-stage juvenile stylets are shorter 19.2 µm compared to 20.5 µm of H. oryzae.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterizations of Heterodera oryzae in Korea

Mwesige

Kim

Park

et al. 2020

Journal of Nematology

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Rice is one of the most important staple grains in Korea and the largest starch source in addition to its usefulness in the production of beverages. Under different areas and environments of production, various pests and diseases including soil-borne plant pathogens such as plant-parasitic nematodes can compromise rice productivity. In a survey to identify plant parasitic nematodes on rice, cyst nematodes were encountered in rice fields that required characterization and identification. Phylogenetic analysis of the LSU D2-D3 region and ITS region could not separate the studied species from Heterodera elachista. However, phylogenetic analysis of the COI gene of the mitochondrial DNA clearly separated H. elachista from the new species into two different clusters. Combining morphology and molecular diagnostics, the species was identified as Heterodera oryzae belonging to the 'Cyperi' group whose cysts are characterized by vulval cones that are ambifenestrate, underbridge present with bullae. Second-stage juveniles have three incisors in the lateral field with long tails and long hyaline region.

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“…Based on classical morphology and host data, H.fici has been variously placed with members of the Schachtii group (Mulvey and Golden 1983, Baldwin and Mundo-Ocampo 1991) or in the Avenae group (Stone 1975). Subbotin et al (2001) and recently De Luca et al (2013), placed H.fici within the Humuli group characterised by bifenestral vulva in most species of the group, few or absent bullae, very weak underbridge, and long vulval slit situated in a cleft between the thickened vulval lips.…”

Section: Introductionmentioning

confidence: 97%

Integrative diagnosis, biological observations, and histopathology of the fig cyst nematode Heterodera fici Kirjanova (1954) associated with Ficus carica L. in southern Italy

Fanelli

Vovlas²,

Santoro³

et al. 2019

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Morpho-biological notes and histopathology, based on LM and SEM observations, of the fig cyst nematode Heteroderafici isolated from Ficuscarica roots, collected in home and public gardens of Apulia region, southern Italy, are described and illustrated. Seventy-five localities throughout the Apulia region were sampled and one-quarter of the sampled localities had fig roots infested with H.fici, with population densities ranging from 44 to 180 cysts/100 ml of soil. All attempts to detect H.fici on ornamental Ficus spp. as well as on imported bonsai in Italy were unsuccessful. Morphometric characters of the Italian population conform to those of the type and re-description populations reported for H.fici. Molecular analysis using ITS, D2–D3 expansion domains of the 28S rRNA, and the partial 18S rRNA sequences of H.fici newly obtained in this study matched well with the corresponding sequences of H.fici present in the GenBank database. Phylogenetic trees confirmed and supported the grouping of H.fici in the Humuli group. Heteroderafici completes its embryogenic development in 14–16 days at 25 °C. Post-invasion development and maturity in the roots of F.carica seedlings is completed in 64–68 days at 25–28 °C with juveniles and adults showing different parasitic habits, being endoparasitic and semi-endoparasitic respectively. The establishment of permanent feeding sites that consist of the formation of large syncytia causes anatomical modification of vascular elements and general disorder in the root stelar structures. Syncytia structures associated with mature females showed different degrees of vacuolisation, numbers of syncytial cells, and contained nuclei and nucleoli which were constantly hypertrophied.

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Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy: integrative diagnosis and bionomics

Cited by 22 publications

References 22 publications

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Morphological and molecular characterizations of Heterodera oryzae in Korea

Integrative diagnosis, biological observations, and histopathology of the fig cyst nematode Heterodera fici Kirjanova (1954) associated with Ficus carica L. in southern Italy

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