AbstraetThe nitrogen-fixing blue-green alga Anabaena ambigua was grown in a medium which contained either ammonium chloride as nitrogen source or molecular nitrogen. In the latter case the alga produced heterocysts. The material was analysed for ascorbic acid, dehydro-ascorbic acid and diketogulotiic acid. The amount of ascorbic acid was found always to be higher in the alga grown with molecular nitrogen. When the alga grown with combined nitrogen was transferred to the medium lacking it, there was an increase in the ascorbic acid content. Conversely, when material cultured on the nitrogenfree medium was suspended in the medium containing ammonium chloride, there •was a decrease in the celiuiar ascorbic acid. Exogenously added ascorbic acid, up to 0.5 mg per ml, increased the heterocyst frequency to nearly three times that of the control. D-isoascorbic acid, an analogue of ascorbic acid, also showed an enhancement of heterocyst production.Algal extracts were fractionated by poiyacrylamide electrophoresis, and the presence of ascorbic acid oxidase was detected on the gels. Two bands, with M values 0.J4 and 1.0, were found to give positive test for the enzyme. The total enzyme activity was 16.7 "/ty higher in cells grown with molecular nitrogen than in those gro'wn Tvith combined nitrogen. The exact location of tbe enzyme in the alga ist not known although the heterocysts were earlier shown to contain ascorbic acid. Cytochemical tests, however, indicated strong peroxidase activity in tbe heterocysts.