Heterobasidion annosum 5.8s ribosomal DNA and internal transcribed spacer sequence: rapid identification of European intersterility groups by ribosomal DNA restriction polymorphism

Kasuga, Takao; Woods, Caroline; Woodward, Steven; Mitchelson, Keith

doi:10.1007/bf00351853

Cited by 43 publications

(35 citation statements)

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“…We also observed a greater accumulation of iron in P. sylvestris roots in response to exposure to H. annosum s.s. than in response to the other two species . Heterobasidion parviporum and H. abietinum are genetically related to each other more closely than to H. annosum s. s. (Karjalainen 1996;Kasuga et al 1993), which was confirmed by evolutionary studies by Dalman et al (2010). This can explain the similar response of the host to H. parviporum and H. abietinum.…”

Section: Discussionmentioning

confidence: 55%

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

et al. 2015

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Abstract:We examined the interaction between the roots of Pinus sylvestris and closely related species Heterobasidion annousum s.l. (H. annosum s.s., H. parviporum, H. abietinum) that differ in host plant preference. The aim of the current study was to determine in roots the accumulation pattern of low molecular mass compounds such as catecholate and hydroxamate derivates, oxalic acid as well as iron-reduction ability of that low molecular mass compounds, that play important roles in wood degradation and they are also involved in pathogenesis. The accumulation of catechol and hydroxamate derivates increased during the early (2-6 h) and late (24-48 h) stages of interaction and similar pattern of oxalic acid accumulation were observed. The level of catecholate derivates in P. sylvestris roots that were challenged with H. parviporum or H. abietinum correlated strongly with iron reducing ability. However, when host was exposed to H. annosum s. s. hydroxamates rather than catecholates regulated iron reducing ability. The extracellular Fe 3+ reducing activity was greater for H. annosum s. s. isolates than for isolates of two other species, and reduction of ferric iron may promote oxidative burst in host cell and fungal colonization. Catecholate concentration in the presence of H. annosum s.s. contributing to host cell death, confirm iron involvement in infection success.

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Section: Discussionmentioning

confidence: 55%

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

et al. 2015

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“…Thus, no ITS polymorphisms have been observed within the European H. annosum s.s. and H. abietinum, while a relatively low degree of variation has been found within the European H. parviporum and North American S and P intersterility groups (Kasuga et al 1993, Harrington et al 1998), as well as within H. araucariae (Harrington et al 1998) and H. insulare . During study I, the ITS alleles of Chinese H. annosum s.l.…”

Section: Its Rdna Markers (I Ii)mentioning

confidence: 99%

“…species complex was long regarded as a single cosmopolitan species with a wide host range, but mating experiments have revealed the existence of five separate Heterobasidion species showing differences in their host tree preferences (Korhonen 1978a, Chase and Ullrich 1988, Capretti et al 1990, Korhonen et al 1992, Niemelä and Korhonen 1998. Differentiation between these biological species has been confirmed using numerous different molecular approaches including isoenzyme analysis (Karlsson and Stenlid 1991, Otrosina et al 1993, Maijala et al 1995, Goggioli et al 1998), fatty acid and sterol profiles (Müller et al 1995), and various genetic marker molecules (Fabritius and Karjalainen 1993, Kasuga et al 1993, Stenlid et al 1994, La Porta et al 1997, Garbelotto et al 1998, Goggioli et al 1998, Vainio et al 1999, Kasuga and Mitchelson 2000, Johannesson and Stenlid 2003, Maijala et al 2003.…”

Section: Heterobasidion Annosum Species Complexmentioning

confidence: 99%

“…Based on this, the ITS and/or IGS molecules have been used for phylogenetic and population studies among many wood-associated fungal species (Hallenberg et al 1996, Harrington et al 1998, Kasuga and Mitchelson 2000, James et al 2001, Kauserud and Schumacher 2003a, 2003b. The level of intraspecific sequence polymorphism varies greatly between fungal taxa, which is also evident for P. gigantea and H. annosum s.l.…”

Section: The Ribosomal Rna Gene Clustermentioning

confidence: 99%

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Ecological impacts of Phlebiopsis gigantea biocontrol treatment against Heterobasidion spp. as revealed by fungal community profiling and population analyses

Vainio¹

2008

Diss. For.

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Wood decay fungi belonging to the species complex Heterobasidion annosum sensu lato are among the most common and economically important species causing root rot and stem decay in conifers of the northern temperate regions. New infections by these pathogens can be suppressed by tree stump treatments using chemical or biological control agents. In Finland, the corticiaceous fungus Phlebiopsis gigantea h a s b e e n f o r m u l a t e d i n t o a commercial biocontrol agent called Rotstop (Verdera Ltd.).This thesis addresses the ecological impacts of Rotstop biocontrol treatment on the mycoflora of conifer stumps. Locally, fungal communities within Rotstop-treated and untreated stumps were analyzed using a novel method based on DGGE profiling of small subunit ribosomal DNA fragments amplified directly from wood samples. Population analyses for P. gigantea and H. annosum s.l. were conducted to evaluate possible risks associated with local and/or global distribution of the Rotstop strain.Based on molecular community profiling by DGGE, we detected a few individual wood-inhabiting fungal species (OTUs) that seemed to have suffered or benefited from the Rotstop biocontrol treatment. The DGGE analyses also revealed fungal diversity not retrieved by cultivation and some fungal sequence types untypical for decomposing conifer wood. However, statistical analysis of DGGE community profiles obtained from Rotstoptreated and untreated conifer stumps revealed that the Rotstop treatment had not caused a statistically significant reduction in the species diversity of wood-inhabiting fungi within our experimental forest plots.Locally, ISSR genotyping of cultured P. gigantea strains showed that the Rotstop biocontrol strain was capable of surviving up to six years within treated Norway spruce stumps, while in Scots pine stumps it was sooner replaced by successor fungal species. In addition, the spread of resident P. gigantea strains into Rotstop-treated forest stands seemed effective in preventing the formation of genetically monomorphic populations in the short run. On a global scale, we detected a considerable level of genetic differentiation between the interfertile European and North American populations of P. gigantea. These results strongly suggest that local biocontrol strains should be used in order to prevent global spread of P. gigantea and hybrid formation between geographically isolated populations.The population analysis for H. annosum s.l. revealed a collection of Chinese fungal strains that showed a high degree of laboratory fertility with three different allopatric H. annosum s.l. taxa. However, based on the molecular markers, the Chinese strains could be clearly affiliated with the H. parviporum taxonomical cluster, which thus appears to have a continuous distribution range from Europe through southern Siberia to northern China.

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“…The relative humidity was set at maximum (95%) with an 18 h, 18 °C day and 6 h, (Kasuga et al, 1993).…”

Section: Tree Seedlingsmentioning

confidence: 99%

Chitinolytic activities of Eucalyptus pilularis and Pinus sylvestris root systems challenged with mycorrhizal and pathogenic fungi

1995

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SUMM.ARYThe chitinolytic activities of Pinus sylvestris L. and Eucalyptus pilularis Sm. roots were measured after an exposure for 24 h to either ectotnycorrhizal {Pisolithus tinctorius (Pers.) Coker and Couch) or root-infecting pathogenic fungi {Heterobasidiuri annosum (Fr.) Karst. and Phytophthura cinnamomi Rands,). P. sylveslris roots not exposed to fungal hyphae had low levels of/?-.V-acetylglucosaminidase. This activity greatly increased after exposure to H, annosum but not P. linnamomi or P. tinctorius. /?-A'-AcetyIglucosaminidase activity in E. pilularis roots vi'as unchanged after exposure to P. cinnamomi or P. tinctorius. Exo-and endochitinase activity increased after P. sylvestris roots were challenged with H. annosum, but not by the other fungi. These results suggest that /?-A'-acetylglucosaminidase might be an important early response to chitinous pathogens.

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Heterobasidion annosum 5.8s ribosomal DNA and internal transcribed spacer sequence: rapid identification of European intersterility groups by ribosomal DNA restriction polymorphism

Cited by 43 publications

References 14 publications

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

Ecological impacts of Phlebiopsis gigantea biocontrol treatment against Heterobasidion spp. as revealed by fungal community profiling and population analyses

Chitinolytic activities of Eucalyptus pilularis and Pinus sylvestris root systems challenged with mycorrhizal and pathogenic fungi

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