Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

Mucha, Joanna; Guzicka, Marzenna; Łakomy, Piotr; Zadworny, Marcin

doi:10.12657/denbio.073.010

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…used in the current study. In addition, levels of oxalic acid were correlated negatively with cell death (Mucha et al 2015). This may suggest that oxalic acid is used as a source of H 2 O 2 , since oxalic acid is a substrate for oxalate oxidase, which releases H 2 O 2 and CO 2 from oxalic acid (Zhang et al 1995).…”

Section: Discussionmentioning

confidence: 99%

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

et al. 2015

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Reactive oxygen species (ROS) can be promoted differently, although iron is regarded as a condicio sine qua of their generation. We assessed how enzymes involved in ROS production and iron accumulation impact the interactions between Pinus sylvestris root and p at ho g e ns w i t h di ff e r e n t ho s t pr ef e r e n ce s (Heterobasidion annosum sensu stricto, H. parviporum and H. abietinum). Studies with inhibitors of ROS generation showed that diphenylene iodonium (DPI) and potassium cyanide (KCN) decreased the mortality of P. sylvestris root cells when exposed to H. parviporum in the elongation zone. In the meristematic zone, inhibited production of NADPH oxidase and oxalic acid decreased mortality of host root cells challenged with H. parviporum or H. abietinum. In the meristematic zone, iron immobilization with desferoxamine (DFO), inhibited percentage of dead host cells exposed to H. annosum s.s. Scots pine inoculation with high compatible H. annosum s.s., irrespectively in the root zone, led to an accumulation of superoxides (O 2 − ) and a colocalisation of H 2 O 2 with ferric or ferrous ions involved in production of the most harmful ROS -hydroxyl radicals at 4 h. After 48 h of mutual interaction between P. sylvestris and H. annousum s.s, a similar pattern as at 4 h was found in the root meristematic zone. We also showed that O 2 − accumulation in the P. sylvestris -H. annosum s.s. pathosystem was not NADPH oxidase dependent. During the interaction of P. sylvestris and H. annosum s.s., colocalisation of H 2 O 2 with Fe 2+ and Fe 3+ decreased by disturbing ROS production; this pattern was less apparent when H. parviporum or H. abietinum were identified as the invader. Our work implies that different mechanisms related to plant cell death can operate in both the root elongation and meristematic zone of P. sylvestris colonized with H. annosum s.s. pathosystem.

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Section: Discussionmentioning

confidence: 99%

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

et al. 2015

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“…Previous studies have demonstrated that iron plays a role in fungal-host interactions in both leaves [1,2,3] and roots [4,5,6,7,8]. Thus, metal-binding siderophores secreted by fungi, which regulate iron distribution, play an important role in virulence [1,3,5] and in maintaining the mutualistic interaction of mycorrhizae [9].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells

Mucha

Pawłowski

Klupczyńska

et al. 2019

IJMS

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Protein acetylation affects gene expression, as well as other processes in cells, and it might be dependent on the availability of the metals. However, whether iron chelating compounds (siderophores) can have an effect on the acetylation process in plant roots is largely unknown. In the present study, western blotting and confocal microscopy was used to examine the degree of acetylation of histone H3 and alpha tubulin in Pinus sylvestris root cells in the presence of structurally different siderophores. The effect of metabolites that were produced by pathogenic and mycorrhizal fungi was also assessed. No effect was observed on histone acetylation. By contrast, the metabolites of the pathogenic fungus were able to decrease the level of microtubule acetylation, whereas treatment with iron-free ferrioxamine (DFO) was able to increase it. This latter was not observed when ferrioxamine-iron complexes were used. The pathogen metabolites induced important modifications of cytoskeleton organization. Siderophores also induced changes in the tubulin skeleton and these changes were iron-dependent. The effect of siderophores on the microtubule network was dependent on the presence of iron. More root cells with a depolymerized cytoskeleton were observed when the roots were exposed to iron-free siderophores and the metabolites of pathogenic fungi; whereas, the metabolites from mycorrhizal fungi and iron-enriched forms of siderophores slightly altered the cytoskeleton network of root cells. Collectively, these data indicated that the metabolites of pathogenic fungi mirror siderophore action, and iron limitation can lead to enhanced alternations in cell structure and physiology.

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The effects of structurally different siderophores on the organelles of Pinus sylvestris root cells

Mucha

Gabała

Zadworny

2019

Planta

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Main conclusion Siderophores are a driver of Pinus sylvestris root responses to metabolites secreted by pathogenic and mycorrhizal fungi.Structurally different siderophores regulate the uptake of Fe by microorganisms and may play a key role in the colonization of plants by beneficial or pathogenic fungi. Siderophore action, however, may be dependent on the distribution of Fe within cells. Here, the involvement of siderophores in determining the changes of organelle morphology and element composition of some cellular fractions of root cells in Pinus sylvestris to trophically diverse fungi was investigated. Changes in the morphology and concentrations of different elements within organelles of root cells in response to three structurally different siderophores were examined by transmission electron microscopy combined with energy-dispersive X-ray spectroscopy. Weak development of mitochondrial cristae and the deposition of backup materials in plastids occurred in the absence of Fe in the structures of triacetylfusarinine C and ferricrocin. In response to metabolites of both pathogenic and mycorrhizal fungi, Fe accumulated mainly in the cell walls and cytoplasm. Fe counts increased in all of the analyzed organelles in response to applications of ferricrocin and triacetylfusarinine C. Chelation of Fe within the structure of siderophores prevents the binding of exogenous Fe, decreasing the abundance of Fe in the cell wall and cytoplasm. The concentrations of N, P, K, Ca, Mn, Cu, Mg, and Zn also increased in cells after applications of ferricrocin and triacetylfusarinine C, while the levels of these elements decreased in the cell wall and cytoplasm when Fe was present within the structure of the siderophores. These results provide insight into the siderophore-driven response of plants to various symbionts.

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Accumulation of iron-binding compounds in root of Pinus sylvestris challenged by Heterobasidion annosum sensu lato

Cited by 3 publications

References 36 publications

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells

The effects of structurally different siderophores on the organelles of Pinus sylvestris root cells

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