Hetero-exopolysaccharide from the extremely halophilic Halomonas smyrnensis K2: production, characterization and functional properties in vitro

Joulak, Ichrak; Finore, Ilaria; Poli, Annarita; Abid, Yousra; Bkhairia, Intidhar; Nicolaus, Barbara; Donato, Paola Di; Poggetto, Giovanni Dal; Gharsallaoui, Adem; Attia, Hamadi; Azabou, Samia

doi:10.1007/s13205-020-02356-5

Cited by 11 publications

(5 citation statements)

References 56 publications

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“…The peak flocculation activity of these EPS in this study was achieved at the similar level of dosage with those of several reported strains, including Leuconostoc mesenteroides strain XR1 (Wang et al, 2021), Streptococcus thermophilus ZJUIDS-2-01 (Cao et al, 2021). Furthermore, the peak flocculation activity of the three types of purified EPS were much higher than those from strains Halomonas elongata S6 (Joulak et al, 2020), Pseudoalteromonas sp. SM9913.…”

Section: A B C Dsupporting

confidence: 83%

“…The chemical structure of EPS plays important roles in the biological functions. The activity of EPS was considered to be related to the molecular weight (Mw), anomeric configuration, and monosaccharide composition (Guo et al, 2010;Joulak et al, 2020;Vinothkanna et al, 2021). However, the microbial isolates from marine environment are still not enough, and the biological function with the structure of EPS needs further study.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud

Feng

Qian²,

Yang³

et al. 2023

Front. Microbiol.

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Three novel types of exopolysaccharides (EPS) EPS-S8, EPS-S5, and EPS-F10 were extracted and purified from bacterial isolates Bacillus sp. GHS8, Pseudoalteromonas sp. GHS5 and Psychrobacter sp. GHF10, which were originated from natural bioflocculant of Ruditapes philippinarum conglutination mud (RPM), respectively. The EPS had similar function groups C-H, N-H, C-O, and C = O. The EPS were composed of different monosaccharides (EPS-F10, Man: GlcN: GlcUA: GalUA = 1:0.66:5.75:0.51; EPS-S5, Man: Gal: GlcN: Rib = 1: 0.50: 2.94: 0.26; EPS-S8, Man: Gal: GlcN = 1:1.54:7.69). The molecular weights (Mw) of EPS were ordered as 51.4 kDa (EPS-S5) > 9.15 kDa (EPS-S8) > 4.41 kDa (EPS-F10). Three types of EPS all showed higher peak flocculation activities than the reported crude EPS from the RPM. Besides, the EPS also exhibited efficient decoloration and antioxidation activities, especially for EPS-S8, which might be due to the low Mw and specific monosaccharide composition.

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Section: A B C Dsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud

Feng

Qian²,

Yang³

et al. 2023

Front. Microbiol.

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“…From the HPLC-RID data in Figure 2, it is apparent that nEPS produced by the strain G11 is an heteropolysaccharide containing two monomers, rhamnose and fructose. The most interesting aspect of Figure 2 is the detection of fructose, for the first time to the best of our knowledge, in the composition of EPSs produced by Halomonas strains; contrarily, rhamnose is present in the composition of EPSs synthesized by different strains of Halomonas [45,50,52].…”

Section: This Workmentioning

confidence: 90%

“…As shown in Table 2, the MW of the present nEPS was comparable to those synthesized by H. anticarinesis FP36 [47], H. almeriensis M8 T [45], and H. saliphila LCB169 T [53] and lower than the MW of EPS produced by Halomonas sp. MCTG39a [51] and H. smyrnensis K2 [52]. Strikingly, both iEPSs-containing fragments F1 and F2 (Figure 3) obtained by gamma irradiation with 1.0 and 1.5 kGy displayed the lowest MW.…”

Section: This Workmentioning

confidence: 96%

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Chouchane¹,

Boutiti²,

Ouertani³

et al. 2021

Polymers

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In this work, a native exopolysaccharide (nEPS) produced by Halomonas desertis G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (p < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies.

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“…Mining industries are primarily responsible for massive metal waste generation ( Gupta and Diwan, 2017 ; Gayathri et al, 2021 ). For metal biosorption, bacterial EPSs have already demonstrated significant capability to salvage the environment from hazardous heavy metal-containing wastewater, the basis of which is polyanionic bacterial EPSs interacting with positively charged metal ions or ionic exchange mechanism ( Joulak et al, 2020b ). Size, ionic nature, and charge activity of single or multiple metal ion solutions regulate its interaction with anionic bacterial EPSs ( Gupta and Diwan, 2017 ; Zhao et al, 2020 ).…”

Section: Application Of Extremophilic Eps For Environment Bioremediationmentioning

confidence: 99%

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

et al. 2021

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Various microorganisms thrive under extreme environments, like hot springs, hydrothermal vents, deep marine ecosystems, hyperacid lakes, acid mine drainage, high UV exposure, and more. To survive against the deleterious effect of these extreme circumstances, they form a network of biofilm where exopolysaccharides (EPSs) comprise a substantial part. The EPSs are often polyanionic due to different functional groups in their structural backbone, including uronic acids, sulfated units, and phosphate groups. Altogether, these chemical groups provide EPSs with a negative charge allowing them to (a) act as ligands toward dissolved cations as well as trace, and toxic metals; (b) be tolerant to the presence of salts, surfactants, and alpha-hydroxyl acids; and (c) interface the solubilization of hydrocarbons. Owing to their unique structural and functional characteristics, EPSs are anticipated to be utilized industrially to remediation of metals, crude oil, and hydrocarbons from contaminated wastewaters, mines, and oil spills. The biotechnological advantages of extremophilic EPSs are more diverse than traditional biopolymers. The present review aims at discussing the mechanisms and strategies for using EPSs from extremophiles in industries and environment bioremediation. Additionally, the potential of EPSs as fascinating biomaterials to mediate biogenic nanoparticles synthesis and treat multicomponent water contaminants is discussed.

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Hetero-exopolysaccharide from the extremely halophilic Halomonas smyrnensis K2: production, characterization and functional properties in vitro

Cited by 11 publications

References 56 publications

Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud

Characteristics of exopolysaccharides produced by isolates from natural bioflocculant of Ruditapes philippinarum conglutination mud

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

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