Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor

Zheng, Wanli; Teng, Jun; Cheng, Lin; Ye, Yingwang; Pan, Daodong; Wu, Jingjing; Xue, Feng; Liu, Guodong; Chen, Wei

doi:10.1016/j.bios.2016.01.091

Cited by 100 publications

(39 citation statements)

References 35 publications

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“…Generally, analysis of AFB 1 has been achieved by instrumental analysis, [14][15][16] enzyme-linked immunosorbent assay (ELISA) 17,18 and immunochromatographic assay. [19][20][21][22] Immunoassays have been shown to be simple, highly sensitive, cost effective and suitable for high-throughput screening analysis.…”

Section: Introductionmentioning

confidence: 99%

Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed

Yao

Wang

et al. 2017

J Sci Food Agric

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Section: Introductionmentioning

confidence: 99%

Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed

Yao

Wang

et al. 2017

J Sci Food Agric

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“…Presently, to effectively avoid the inherent drawbacks of signal-off biosensors, the integration of different amplification approaches became attractive, such as in vitro DNA amplification. Based on the above facts, an enzyme-based signal amplification electrochemical aptasensing platform for ultrasensitive detection of AFB1 has been reported by Zheng et al [82]. In this work, a heteroenzyme-based two-round signal amplification electrochemical aptasensor approach was designed for AFB1 detection.…”

Section: Electrochemical Aptasensors For Afs Detectionmentioning

confidence: 94%

“…Chemosensors 2017, 5, 1 9 of 14 aptasensing platform for ultrasensitive detection of AFB1 has been reported by Zheng et al [82]. In this work, a heteroenzyme-based two-round signal amplification electrochemical aptasensor approach was designed for AFB1 detection.…”

Section: Electrochemical Aptasensors For Afs Detectionmentioning

confidence: 95%

“…The AFs are subject to light degradation; therefore, the samples must be protected from daylight and standards must be stored in amber-colored vials. For aqueous solutions of AFs, aptasensing platform for ultrasensitive detection of AFB1 has been reported by Zheng et al [82]. In this work, a heteroenzyme-based two-round signal amplification electrochemical aptasensor approach was designed for AFB1 detection.…”

Section: Safety Notesmentioning

confidence: 99%

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Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

et al. 2016

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Abstract:Mycotoxin are small (MW~700 Da), toxic secondary metabolites produced by fungal species that readily colonize crops and contaminate them at both pre-and post-harvesting. Among all, aflatoxins (AFs) are mycotoxins of major significance due to their presence in common food commodities and the potential threat to human health worldwide. Based on the severity of illness and increased incidences of AFs poisoning, a broad range of conventional and analytical detection techniques that could be useful and practical have already been reported. However, due to the variety of structural analogous of these toxins, it is impossible to use one common technique for their analysis. Numerous recent research efforts have been directed to explore alternative detection technologies. Recently, immunosensors and aptasensors have gained promising potential in the area of sample preparation and detection systems. These sensors offer the advantages of disposability, portability, miniaturization, and on-site analysis. In a typical design of an aptasensor, an aptamer (ssDNA or RNA) is used as a bio-recognition element either integrated within or in intimate association with the transducer surface. This review paper is focused on the recent advances in electrochemical immunoand aptasensing platforms for detection of AFs in real samples.

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“…In some aptasensors, biochemical amplification previously developed for DNA sensors devoted to hybridization detection was used like the telomerase/EXO III amplification cycle [149]. This approach increases the time and cost of measurement but decreases the concentrations detected by more than one order of magnitude against conventional analogs.…”

Section: Mycotoxinmentioning

confidence: 99%

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

Evtugyn

Hianik

2019

Chemosensors

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Modern analysis of food and feed is mostly focused on development of fast and reliable portable devices intended for field applications. In this review, electrochemical biosensors based on immunological reactions and aptamers are considered in the determination of mycotoxins as one of most common contaminants able to negatively affect human health. The characteristics of biosensors are considered from the point of view of general principles of bioreceptor implementation and signal transduction providing sub-nanomolar detection limits of mycotoxins. Moreover, the modern trends of bioreceptor selection and modification are discussed as well as future trends of biosensor development for mycotoxin determination are considered.

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Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor

Cited by 100 publications

References 35 publications

Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed

Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

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Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor

Cited by 100 publications

References 35 publications

Eu3+‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B1 in feed

Eu3+‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B1 in feed

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

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Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed

Eu³⁺‐labeled IgG‐based time‐resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B₁ in feed