Hesperidin protects testicular and spermatological damages induced by cisplatin in rats

Abstract

The clinic usage of cisplatin, an anticancer drug, is limited due to it has many side effects in many systems and organs. In this context, it was aimed to investigate the protective effect of hesperidin, a citrus flavonoid, on testicular and spermatological damages induced by cisplatin in rats. The rats were randomly divided into four groups. The first group was kept as a control. In the second groups, cisplatin was given at the single dose of 7 mg kg(-1) intraperitoneally. In the third group, hesperidin was orally administered at the dose of 50 mg/kg day(-1) for 14 days. In the fourth group, cisplatin and hesperidin were given together at the same doses. Cisplatin treatment caused significant reductions enzymatic (SOD, CAT and GPx) and nonenzymatic (GSH) antioxidants and significant induction level of TBARS. In addition, cisplatin treatment caused decreased sperm motility, epididymal sperm concentration, increased abnormal sperm rate and histopathological damage. In contrast, hesperidin treatment significantly attenuated the harmful effects. In conclusion, this study clearly demonstrated that hesperidin has protective effects on cisplatin-induced reproductive system toxicity depending on its antioxidant properties. Thus, it is thought that hesperidin may be useful against cisplatin toxicity in patients with cancer in terms of reproductive system.

Investigation of the effects of curcumin, vitamin E and their combination in cisplatin-induced testicular apoptosis using immunohistochemical technique

“…[1,2] However, its ototoxicity, nephrotoxicity, myelotoxicity, and gastrointestinal toxicity, and serious side effects involving neurologic, hematologic, and reproductive system restrict its clinical use. [3,4] Multiple number of studies have indicated the roles of reactive oxygen radicals (ROSs), and oxidative stress (OS) on reproductive toxicity of cisplatin. [4] Though oxygen is indispensable for the survival of living organisms, during its metabolism, extremely reactive intermediate products which are known as the sources of free radicals are formed.…”

“…[3,4] Multiple number of studies have indicated the roles of reactive oxygen radicals (ROSs), and oxidative stress (OS) on reproductive toxicity of cisplatin. [4] Though oxygen is indispensable for the survival of living organisms, during its metabolism, extremely reactive intermediate products which are known as the sources of free radicals are formed. Reactive molecules released during conversion of foods into energy are called as free radicals.…”

“…[5] In various studies performed with rat testes, it has been demonstrated that cisplatin treatment leads to a marked increase in apoptosis of germ cells, histopathological damage, and decrease in levels of biochemical antioxidant enzymes including SOD, GPX, CAT, and GSH. [3,4,7] Cisplatin is very widely used in the cancer treatment either as a monotherapy or combination therapy with other agents. [8,9] Despite promising results especially in the treatment of testicular malignancy, long-lasting testicular dysfunction is one of the widely seen adverse effects of cisplatin.…”

“…Some studies performed have demonstrated protective effects of antioxidant substances on cisplatin-induced toxicity. [4,7] Curcumin is a polyphenol isolated from the roots of the plant turmeric. Antioxidant, anti-free radical, antineoplastic, and antifungal effects of curcumin have been reported.…”

Ratlarda Metoraksat Kaynaklı Testiküler Toksisite Üzerine Hesperidinin Etkisi

“…HP has radical clearance and anti-inflammatory effects [15,16] . It has been reported that Hesperidin significantly reduces lipid peroxidation in testicular tissue, leads to positive effects on sperm parameters and biochemical parameters, as well as provides improvements in epididymal functions [14,17] .…”