Hesperidin-loaded nanoemulsions improve cytotoxicity, induce apoptosis, and downregulate miR-21 and miR-155 expression in MCF-7

Magura, Judie; Hassan, Daniel; Moodley, Roshila; Mackraj, Irene

doi:10.1080/02652048.2021.1979673

Cited by 11 publications

(6 citation statements)

References 53 publications

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“…In another study, the cytotoxicity of hesperidin was evaluated against the MCF-7 cell lines and the IC 50 value was calculated 100–140 μg/mL [ 39 ]. Also, hesperidin-loaded PLGA nanoparticles and hesperidin-loaded nano-emulsions have improved the cytotoxicity effect of hesperidin [ 40 , 41 ]. However, using the nanoliposome composed of soy lecithin for loading hesperidin has not been investigated.…”

Section: Resultsmentioning

confidence: 99%

Experimental, molecular docking and molecular dynamic studies of natural products targeting overexpressed receptors in breast cancer

Taghizadeh

Niazi‎²,

Moghadam³

et al. 2022

PLoS ONE

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Natural compounds are proper tools for inhibiting cancer cell proliferation. Hence, the search for these ligands of overexpressed receptors in breast cancer has been a competitive challenge recently and opens new avenues for drug discovery. In this research, we have investigated molecular interactions between natural products and overexpressed receptors in breast cancer using molecular docking and dynamic simulation approaches followed by extraction of the best ligand from Citrus limetta and developing for nanoscale encapsulation composed of soy lecithin using a sonicator machine. The encapsulation process was confirmed by DLS and TEM analyses. Anticancer activity was also examined using MTT method. Among the investigated natural compounds, hesperidin was found to bind to specific targets with stronger binding energy. The molecular dynamics results indicated that the hesperidin-MCL-1 complex is very stable at 310.15 K for 200 ns. The RP-HPLC analysis revealed that the purity of extracted hesperidin was 98.8% with a yield of 1.72%. The results of DLS and TEM showed a strong interaction between hesperidin and lecithin with an entrapped efficiency of 92.02 ± 1.08%. Finally, the cytotoxicity effect of hesperidin was increased against the MDA-MB-231 cell line with an IC50 value of 62.93 μg/mL after encapsulation, whereas no significant effect against the MCF10A cell line. We showed for the first time that hesperidin is a flexible and strong ligand for the MCL-1 receptor. Also, it has the in vitro ability to kill the MDA-MB-231 cell lines without having a significant effect on the MCF10A cell lines. Therefore, hesperidin could be used as a food ingredient to generate functional foods.

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Section: Resultsmentioning

confidence: 99%

Experimental, molecular docking and molecular dynamic studies of natural products targeting overexpressed receptors in breast cancer

Taghizadeh

Niazi‎²,

Moghadam³

et al. 2022

PLoS ONE

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“…Cell cycle seizure in the G2/M phase and death of cells through apoptosis were both brought on by treatment with HP-NE, indicating that HP-NE may be used as a therapeutic agent to treat breast cancer. 118 The anticancer effect of optimized NG-loaded showed high stability, and controlled NG release from the NE was followed by an initial burst release. It also exhibited potential anticancer effect studies against A549 lung carcinoma cells.…”

Section: Flavonoids-loaded Nanoemulsion In Cancer Therapymentioning

confidence: 95%

“…They loaded QT into an agarose-polyvinylpyrrolidone (PVP)-hydroxyapatite (HAp) hydrogel nanocomposite, increased loading efficiency by up to 61%, encapsulated it within W/O/W Nes, and exhibited potential cell apoptosis against the MCF-7 human breast cancer cell line. 117 Hesperidin (HP)-loaded NEs (HP-NE) were prepared by Magura et al, 118 via spontaneous emulsification to improve the solubility, bioavailability, and efficacy of hesperidin for treating breast cancer using MCF-7 cell lines. Cell cycle seizure in the G2/M phase and death of cells through apoptosis were both brought on by treatment with HP-NE, indicating that HP-NE may be used as a therapeutic agent to treat breast cancer.…”

Section: Flavonoids-loaded Nanoemulsion In Cancer Therapymentioning

confidence: 99%

“…Hesperidin (HP)-loaded NEs (HP-NE) were prepared by Magura et al, via spontaneous emulsification to improve the solubility, bioavailability, and efficacy of hesperidin for treating breast cancer using MCF-7 cell lines. Cell cycle seizure in the G2/M phase and death of cells through apoptosis were both brought on by treatment with HP-NE, indicating that HP-NE may be used as a therapeutic agent to treat breast cancer . The anticancer effect of optimized NG-loaded showed high stability, and controlled NG release from the NE was followed by an initial burst release.…”

Section: Polyphenols-loaded Nanoemulsion In Cancer Therapymentioning

confidence: 99%

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Therapeutic Implications of Dietary Polyphenols-Loaded Nanoemulsions in Cancer Therapy

Tomar,

Das,

Balaga

et al. 2024

ACS Appl. Bio Mater.

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Cancer is one of the major causes of death worldwide, even the second foremost cause related to non-communicable diseases. Cancer cells typically possess several cellular and biological processes including, persistence, propagation, differentiation, cellular death, and expression of cellular-type specific functions. The molecular picture of carcinogenesis and progression is unwinding, and it appears to be a tangled combination of processes occurring within and between cancer cells and their surrounding tissue matrix. Polyphenols are plant secondary metabolites abundant in fruits, vegetables, cereals, and other natural plant sources. Natural polyphenols have implicated potential anticancer activity by various mechanisms involved in their antitumor action, including modulation of signaling pathways majorly related to cellular proliferation, differentiation, relocation, angiogenesis, metastatic processes, and cell death. The applications of polyphenols have been limited due to the hydrophobic nature and lower oral bioavailability that could be possibly overcome through encapsulating them into nanocarrier-mediated delivery systems, leading to improved anticancer activity. Nanoemulsions (NEs) possess diverse feasible properties, including greater surface area, modifiable surficial charge, higher half-life, site-specific targeting, and formulation imaging capability necessary to create a practical therapeutic impact, and have drawn increased attention in cancer therapy research. This review has summarized and discussed the basic concepts, classification, delivery approaches, and anticancer mechanism of various polyphenols and polyphenols-encapsulated nanoemulsions with improved cancer therapy.

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“…With this aim, the effect of HPNEM on MCF-7 cell cycle arrest, apoptosis, and the expression of oncomiRs (miR-155 and miR-21) were evaluated. The expression of miR-155 was downregulated by HPNEM, supporting its therapeutic potential in treating breast cancer [ 112 ].…”

Section: Lipid-based Carrier Systemsmentioning

confidence: 99%

Lipid-Based Delivery Systems for Flavonoids and Flavonolignans: Liposomes, Nanoemulsions, and Solid Lipid Nanoparticles

et al. 2023

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Herbal chemicals with a long history in medicine have attracted a lot of attention. Flavonolignans and flavonoids are considered as two classes of the above-mentioned compounds with different functional groups which exhibit several therapeutic capabilities such as antimicrobial, anti-inflammatory, antioxidant, antidiabetic, and anticancer activities. Based on the studies, high hydrophobic properties of the aforementioned compounds limit their bioavailability inside the human body and restrict their wide application. Nanoscale formulations such as solid lipid nanoparticles, liposomes, and other types of lipid-based delivery systems have been introduced to overcome the above-mentioned challenges. This approach allows the aforementioned hydrophobic therapeutic compounds to be encapsulated between hydrophobic structures, resulting in improving their bioavailability. The above-mentioned enhanced delivery system improves delivery to the targeted sites and reduces the daily required dosage. Lowering the required daily dose improves the performance of the drug by diminishing its side effects on non-targeted tissues. The present study aims to highlight the recent improvements in implementing lipid-based nanocarriers to deliver flavonolignans and flavonoids.

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Hesperidin-loaded nanoemulsions improve cytotoxicity, induce apoptosis, and downregulate miR-21 and miR-155 expression in MCF-7

Cited by 11 publications

References 53 publications

Experimental, molecular docking and molecular dynamic studies of natural products targeting overexpressed receptors in breast cancer

Experimental, molecular docking and molecular dynamic studies of natural products targeting overexpressed receptors in breast cancer

Therapeutic Implications of Dietary Polyphenols-Loaded Nanoemulsions in Cancer Therapy

Lipid-Based Delivery Systems for Flavonoids and Flavonolignans: Liposomes, Nanoemulsions, and Solid Lipid Nanoparticles

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