Hesperetin Liposomes for Cancer Therapy

Abstract

Hesperetin is a compound from citrus fruit that has previously been found to exert anticancer activity through a variety of mechanisms. However, the application of hesperetin to cancer therapy has been hampered by its hydrophobicity, necessitating the use of toxic solubilizing agents. Here, we have developed the first liposome-based delivery system for hesperetin. Liposomes were fabricated using the thin-layer evaporation technique and physical and pharmacological parameters were measured. The liposomes remained stable for prolonged periods of time in serum and under storage conditions, and displayed anticancer efficacy in both H441 lung cancer cells and MDA-MB-231 breast cancer cells. Furthermore, the anticancer activity was not impaired in cells expressing the multidrug resistance protein 1 (MDR-1). In conclusion, the encapsulation of hesperetin in liposomes does not interfere with therapeutic efficacy and provides a biocompatible alternative to toxic solubilizing agents, thereby enabling future clinical use of this compound for cancer therapy.

A Liposome Encapsulated Ruthenium Polypyridine Complex as a Theranostic Platform for Triple-Negative Breast Cancer

“…These results are supported by previous studies, which demonstrate that liposomes of similar composition do not cause toxicity. 32–34 The in vitro anticancer activity of Lipo-Ru in MDA-MB-231 cells after 24 h, 48 h, and 72 h was also determined with an MTT assay. The results reveal a dramatic difference in the ability of Ru and Lipo-Ru to reduce cell viability.…”

Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation

“…Zeta potential measurements were performed as previously described 88. Briefly, BiNPs were diluted in HPLC‐grade water (10 8 BiNPs mL −1 ) or phosphate buffered saline and placed in folded capillary cells (Malvern, USA).…”

Contribution of Kupffer cells to liposome accumulation in the liver

“…Dynamic light scattering and laser Doppler micro-electrophoresis were used to measure the size and zeta potential of liposomes (0.5% v/v in distilled water), respectively, using a Zetasizer Nano ZS (ZEN 3600, Malvern Instruments) as previously reported [18–20]. The fluorescence intensity (Ex/Em of 590 nm/620 nm) of serial dilutions of the liposome stock solution was measured in black clear-bottom 96-well microplates with a Synergy H4 Hybrid Microplate Reader (BioTek).…”