Herpesviruses Detection by Quantitative Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage and Transbronchial Biopsy in Lung Transplant: Viral Infections and Histopathological Correlation

Costa, Cristina; Delsedime, Luisa; Solidoro, Paolo; Curtoni, Antonio; Bergallo, Massimiliano; Libertucci, Daniela; Baldi, S.; Rinaldi, Mauro; Cavallo, Rossana

doi:10.1016/j.transproceed.2010.03.086

Cited by 39 publications

(39 citation statements)

References 13 publications

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“…The detection of CMV in BAL fluid may or may not be indicative of CMV pneumonia (68,73,(141)(142)(143)(144)(145). Shedding of CMV in saliva and respiratory secretions is not uncommon, and the demonstration of CMV DNA in these respiratory samples in the absence of compatible clinical signs and symptoms (or in the absence of biopsy confirmation) is of unclear significance and does not necessarily indicate CMV pneumonia (141).…”

Section: Nucleic Acid-based Methodsmentioning

confidence: 99%

Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation

2013

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SUMMARY The negative impact of cytomegalovirus (CMV) infection on transplant outcomes warrants efforts toward improving its prevention, diagnosis, and treatment. During the last 2 decades, significant breakthroughs in diagnostic virology have facilitated remarkable improvements in CMV disease management. During this period, CMV nucleic acid amplification testing (NAT) evolved to become one of the most commonly performed tests in clinical virology laboratories. NAT provides a means for rapid and sensitive diagnosis of CMV infection in transplant recipients. Viral quantification also introduced several principles of CMV disease management. Specifically, viral load has been utilized (i) for prognostication of CMV disease, (ii) to guide preemptive therapy, (iii) to assess the efficacy of antiviral treatment, (iv) to guide the duration of treatment, and (v) to indicate the risk of clinical relapse or antiviral drug resistance. However, there remain important limitations that require further optimization, including the interassay variability in viral load reporting, which has limited the generation of standardized viral load thresholds for various clinical indications. The recent introduction of an international reference standard should advance the major goal of uniform viral load reporting and interpretation. However, it has also become apparent that other aspects of NAT should be standardized, including sample selection, nucleic acid extraction, amplification, detection, and calibration, among others. This review article synthesizes the vast amount of information on CMV NAT and provides a timely review of the clinical utility of viral load testing in the management of CMV in solid organ transplant recipients. Current limitations are highlighted, and avenues for further research are suggested to optimize the clinical application of NAT in the management of CMV after transplantation.

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Section: Nucleic Acid-based Methodsmentioning

confidence: 99%

Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation

2013

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“…Herpesviruses were detected in CF and non-CF lung tissue, and were previously identified as core constituents of the respiratory virome (15,22). Although herpesviruses have been linked to complications in lung transplant patients, the roles they play in the progression of CF lung disease, if any, are largely unknown (23).…”

Section: Viral Infections Of Host Tissuementioning

confidence: 99%

Case Studies of the Spatial Heterogeneity of DNA Viruses in the Cystic Fibrosis Lung

Willner

Haynes²,

Furlan³

et al. 2012

Am J Respir Cell Mol Biol

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“…The majority of previous reports have investigated these viruses in high risk populations, such as in lung transplant patients (33). There are limited studies that have evaluated HHVs in critically ill pediatric patients (1).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Human Herpes Viruses in Bronchoalveolar Lavage of Critically Ill Children Undergoing Mechanical Ventilation at a Pediatric Intensive Care Unit

Amanati

Karimi

Fahimzad

et al. 2018

Arch Pediatr Infect Dis

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Background: Critically ill children at the intensive care unit frequently develop hospital-acquired pneumonia (HAP). Human herpes viruses (HHVs), primarily or by reactivation may cause bronchopneumonitis in mechanically ventilated patients. The exact prevalence and role of HHVs in morbidity and mortality of pediatric patients undergoing mechanical ventilation is unclear. The current study was conducted to evaluate the prevalence of 5 Herpes viruses by polymerase chain reaction (PCR) method in bronchoalveolar lavage (BAL) samples in critically ill children at a pediatric intensive care unit (PICU). Methods: Overall, 140 bronchoalveolar lavage samples from 83 mechanically ventilated cases were studied. Samples were taken via the mini-BAL technique. Samples were analyzed by qualitative PCR for detection of herpes simplex virus type 1 and 2 (HSV1/HSV2), human herpes virus type 6 and 7 (HHV6/HHV7), Ebstein-Barr virus (EBV), and cytomegalovirus (CMV).

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Herpesviruses Detection by Quantitative Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage and Transbronchial Biopsy in Lung Transplant: Viral Infections and Histopathological Correlation

Cited by 39 publications

References 13 publications

Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation

Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation

Case Studies of the Spatial Heterogeneity of DNA Viruses in the Cystic Fibrosis Lung

Prevalence of Human Herpes Viruses in Bronchoalveolar Lavage of Critically Ill Children Undergoing Mechanical Ventilation at a Pediatric Intensive Care Unit

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