Prevalence of Human Herpes Viruses in Bronchoalveolar Lavage of Critically Ill Children Undergoing Mechanical Ventilation at a Pediatric Intensive Care Unit

Amanati, Ali; Karimi, Abdollah; Fahimzad, Alireza; Shamshiri, Ahmad Reza; Fallah, Fatemeh; Mahdavi, Alireza; Talebian, Mohammad Hassan

doi:10.5812/pedinfect.12685

Cited by 2 publications

(2 citation statements)

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“…Nevertheless, other sample types can be utilized as resources for HHV‐6/7 molecular study. Amanati et al investigated the incidence of HHV6/7 DNA in bronchoalveolar lavage (BAL) samples of critically ill Iranian children and reported an incidence rate of 13.2% for HHV‐6 DNA and 2.4% for HHV‐7 DNA, 27 which is relatively similar to our results and the existing difference is probably due to different sample type and higher age range of patients in their study. This effect of patient age on HHV‐7 incidence can be inferred from our study's significant relation between HHV7 DNA and age.…”

Section: Discussionsupporting

confidence: 89%

Frequency evaluation and molecular characterization of HHV‐6 and HHV‐7 among children under 5 years with fever and skin rash

Keshavarz

Ghasemi

Arjeini

et al. 2023

Journal of Medical Virology

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Skin rash is one of the most common complications during childhood. Viral agents play an essential role in the development of such symptoms. Present study aims to determine the prevalence and genetic variability of Human Herpesvirus 6 and 7 (HHV‐6 and HHV‐7) infections and their subtypes in children under 5 years of age with skin rash and negative for rubella and measles. We used serum and throat swap samples from 196 children with skin rash and fever. ELISA and IFA tests were performed to detect antibodies against HHV6/7. Sequencing was performed using Sanger sequencing, and BioEdit and MEGA10 software were used for sequence analysis. According to the results, 66% and 40% of cases were positive for HHV‐6 IgM and HHV‐7 IgM, respectively. According to the molecular analysis, HHV‐6 Nested‐PCR was positive in 18% of cases, however, HHV‐7 Nested‐PCR was positive in 7.7% of cases. On the other hand, HHV‐6 IgG and HHV‐7 IgG were positive in 91% and 55% of study cases, respectively. For HHV‐6, we found some genetic variabilities resulting in antigenic changes compared to reference strains. HHV‐7 isolates showed no genetic differentiation and had a stable gene sequence. Based on the results, the detection of some cases of HHV6/7 primary infection and the presence of specific symptoms of roseola in the study population needs continuous evaluation of HHV6/7 frequency and distribution, also genetic variabilities of HHV6. This can pave the way for investigating HHV6 immune evasion and vaccine research and studying the relationship between viral genetic variations and other factors like disease severity. Furthermore, it is necessary to determine the relation between HHV6 genetic changes and latent infection to be considered in possible future vaccines and antiviral drug development.

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Section: Discussionsupporting

confidence: 89%

Frequency evaluation and molecular characterization of HHV‐6 and HHV‐7 among children under 5 years with fever and skin rash

Keshavarz

Ghasemi

Arjeini

et al. 2023

Journal of Medical Virology

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“…HHV-7 and HHV-6 can be reactivated in states of immunosuppression or immunodeficiency in the host, leading to symptomatic infections ( 3 ). Amanati et al ( 4 ) performed bronchoalveolar lavage on 83 critically ill ventilated children and estimated prevalence of HSV-1, HHV-6, HHV7, EBV, and HCMV as approximately 2.4, 13.2, 2.4, 7.2, and 2.4%, respectively, by PCR.…”

Section: Discussionmentioning

confidence: 99%

Case report: A patient with HHV-6 and HHV-7 combined with Whipple’s trophoblast infection and streptococcal pneumonia

Chen,

Zhao,

Yang

et al. 2024

Front. Med.

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Adult respiratory distress syndrome due to viral pneumonia occurs predominantly in immunodeficient populations; adult respiratory distress syndrome secondary to human herpesvirus HHV-6 and HHV-7 pneumonia is extremely rare. Whipple’s disease, caused by Tropheryma whipplei, a Gram-positive bacillus and obligate intracellular pathogen, is clinically challenging to diagnose. Whipple’s disease is a chronic multisystem infectious disease caused by T. whipplei, most often affecting the gastrointestinal tract and joints, seldom the lungs. Both pathogens are opportunistic. We report a case of mixed infectious pneumonia in a patient with type 2 diabetes mellitus. The patient presented with dyspnea and intermittent fever. Imaging revealed multiple large patchy consolidations in the left lung. Routine anti-infective therapy was ineffective. Metagenomic next generation sequencing of bronchoalveolar lavage fluid indicated HHV-6 and HHV-7 pneumonia concurrent with T. whipplei and Streptococcus co-infections. Meropenem was administered to improve treatment. This case represents a rare mixed lung infection by multiple uncommon pathogens, and is of particular clinical significance.

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Prevalence of Human Herpes Viruses in Bronchoalveolar Lavage of Critically Ill Children Undergoing Mechanical Ventilation at a Pediatric Intensive Care Unit

Cited by 2 publications

References 46 publications

Frequency evaluation and molecular characterization of HHV‐6 and HHV‐7 among children under 5 years with fever and skin rash

Frequency evaluation and molecular characterization of HHV‐6 and HHV‐7 among children under 5 years with fever and skin rash

Case report: A patient with HHV-6 and HHV-7 combined with Whipple’s trophoblast infection and streptococcal pneumonia

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