Herpesviridae: Herpes Simplex Virus

Lycke, Erik; Jeansson, Stig

doi:10.1007/978-1-4612-3900-0_11

Cited by 3 publications

(2 citation statements)

References 125 publications

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“…Availability of effective transport systems makes viral isolation a realistic approach for diagnosing many clinically significant virus infections including HSV (44), VZV (66), adenoviruses (70), reoviruses (24,68), togaviruses (13), parainfluenza viruses (28), mumps virus (52), RSV (5), influenza viruses (38), bunyaviruses (14), arenaviruses (48), the enteroviruses (including poliovirus, coxsackieviruses, and echoviruses) (37), and rhinoviruses (3).…”

Section: Virus Stability In Transport Media: a Comparisonmentioning

confidence: 99%

Transport of viral specimens.

Johnson

1990

Clinical Microbiology Reviews

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Section: Virus Stability In Transport Media: a Comparisonmentioning

confidence: 99%

Transport of viral specimens.

Johnson

1990

Clinical Microbiology Reviews

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“…Rapid HSV culture of the cervical and vesicle samples was carried out in mink lung cells by an immunoperoxidase staining method, as described elsewhere by Ziegler et al [16]. The HSV cultures of AF were performed in vero cells and in human diploid fibroblasts [17].…”

Section: Methodsmentioning

confidence: 99%

Herpes Simplex Virus DNA in Amniotic Fluid without Neonatal Infection

Alanen

Hukkanen

2000

Clinical Infectious Diseases

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Twenty-one pregnant women were studied to determine the distribution of herpes simplex virus (HSV): 10 had symptomatic genital herpes, including 1 with primary cervical HSV infection, and 11 had asymptomatic genital herpes. Samples from vesicles, the cervix, and amniotic fluid (AF) were analyzed with 2 separate polymerase chain reaction (PCR) tests and with viral culture. For newborns, clinical examinations and pharyngeal HSV cultures were performed, and cord blood IgM antibodies to HSV were measured. HSV DNA was present in the AF of 3 women with symptomatic HSV infection, but all cultures were negative. HSV was detected more often with PCR than with culture, in both vesicle and cervical samples. For the asymptomatic group, all AF samples were negative, whereas 4 cervical samples were positive by PCR (none were positive by culture). All isolates were HSV type 2. All infants were healthy, and none had cord blood IgM antibodies to HSV, including those with PCR-positive AF.

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Transport of viral specimens

Johnson

1990

Clin Microbiol Rev

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The diagnosis of viral infections by culture relies on the collection of proper specimens, proper care to protect the virus in the specimens from environmental damage, and use of an adequate transport system to maintain virus activity. Collection of specimens with swabs that are toxic to either virus or cell culture should be avoided. A variety of transport media have been formulated, beginning with early bacteriological transport media. Certain swab-tube combinations have proven to be both effective and convenient. Of the liquid transport media, sucrose-based and broth-based media appear to be the most widely accepted and used. Studies on virus stability show that most viruses tested are sufficiently stable in transport media to withstand a transport time of 1 to 3 days. Some viruses may withstand longer transport times. In many cases, it is not necessary to store virus specimens in a refrigerator or send them to the laboratory on wet ice or frozen on dry ice. However, the specimen should not be exposed to environmental extremes. Modern viral transport media allow for more effective use of viral culture and culture enhancement techniques for the diagnosis of human viral infections.

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Herpesviridae: Herpes Simplex Virus

Cited by 3 publications

References 125 publications

Transport of viral specimens.

Transport of viral specimens.

Herpes Simplex Virus DNA in Amniotic Fluid without Neonatal Infection

Transport of viral specimens

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