Herpes Simplex Virus Type 1 Infection of Isogenic Epstein-Barr Virus Genome-Negative and -Positive Burkitt's Lymphoma-Derived Cell Lines

Abstract

The Epstein-Barr virus (EBV) genome-negative Burkitt's lymphoma-derived cell lines BJAB and Ramos and their in vitro EBV-converted sublines BJAB-B1, BJAB-A5, BJAB-B95-8, and AW-Ramos were infected with high multiplicities of herpes simplex virus type 1 (HSV-1; 10 to 70 PFU/cell). Cultures were monitored for cell growth and HSV-1 DNA synthesis. EBV-converted BJAB cultures were more permissive for HSV-1 infection than BJAB cultures. Significant cell killing and HSV-1 DNA synthesis were observed during the first 48 h of infection in the EBV-converted BJAB cultures but not in the BJAB cultures. The EBV-converted BJAB-B1 cell line contains an appreciable fraction of EBV-negative cells. Therefore, it was cloned. EBV-positive and -negative cells were identified by using EBV-determined nuclear antigen anti-complement immunofluorescence. Two types of subclones were identified: (i) those which contained both EBV-determined nuclear antigen-positive and -negative cells and (ii) those which contained only EBV-determined nuclear antigen-negative cells. When levels of HSV-1 DNA synthesis were measured in these subclones, it was found that the former were more permissive for HSV-1 infection than the latter. Thus, the presence of the EBV genome in BJAB cells correlates with increased permissiveness of these celLs for HSV-1 during the first 48 h of infection. Nonetheless, persistent HSV-1 infections were established in both BJAB and EBV-converted BJAB-Bl cultures. No differences in extent of permissiveness for HSV-1 infection were found for Ramos and EBV-converted AW-Ramos cells.

Epstein-Barr Virus Stimulates Torque Teno Virus Replication: A Possible Relationship to Multiple Sclerosis

“…Replication on day 21 was similar in both isolates with higher replication in Ramos than in Ramos/EBV cells. A previous report did not detect any difference in the level of permissiveness for an HSV-1 infection between Ramos and EBVconverted Ramos/EBV cells [74].…”

“…Presently, studies on an association between both TTV and EBV infection in lymphocytes of B-cell lymphoma and Hodgkin's lymphoma have been inconclusive [92,93]. An increased permissiveness for infection with herpes simplex virus during the first 48 hours of infection has been demonstrated in EBV-converted, previously negative Burkitt's lymphoma derived cell lines [74]. Therefore the presence of both TTV and EBV in the same B cell is feasible.…”

Characterization of herpes simplex virus persistence in a human T lymphoblastoid cell line

“…Similar cell crises have been reported for persistent infection of mnouse L cells with VSV (26,39) and parvovirus infection of another human T lymphoblastoid (Molt-4) cell line (3). In contrast to these results, HSV persistence in certain human B lymphoblastoid cell lines is apparently not characterized by such crucial periods of enhanced CPE and viral replication (19,28,30,31).…”

An enhancer within the divergent promoter of Epstein-Barr virus responds synergistically to the R and Z transactivators

“…Sample (20), were maintained in Opt medium with 2.5% fetal calf serum. Raji cells and BJAB 107 cells (21,24) were maintained in RPMI 1640 with 10% fetal calf serum. Cells (5 x 106 per sample) were transfected with DEAE-dextran as described previously (16).…”