Herpes simplex virus-specific serum immunoglobulin a: detection in patients with primary or recurrent herpes infections and in healthy adults

Friedman, Maureen G.; Kimmel, Nina

doi:10.1128/iai.37.1.374-377.1982

Cited by 31 publications

(10 citation statements)

References 16 publications

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“…In our antibody-capture assay this type of inhibition is no longer possible since IgA and IgG are separated prior to reaction with antigen. This might also explain why Friedman and Kimmel [1982] found a significant HSV-IgA response in some of their patients with primary HSV infection. Little is known about the persistence of HSV-IgA, but preliminary results suggest that it is an acute-phase antibody which may persist for approximately the same time as HSV-IgM antibody.…”

Section: Discussionmentioning

confidence: 97%

“…As has been stressed, class-specificity of antisera has to be determined since we as well as others [J. Wilhelm, personal communication] found that some commerical antisera to human IgM or IgA, including the one used by Friedman and Kimmel [1982], cross-react with other classes of antibody, particularly with IgG antibody. On the other hand, the differences might also be due to competititve inhibition by specific IgG antibody.…”

Section: Discussionmentioning

confidence: 97%

“…In contrast Friedman and Kimmel [ 19821 found equal levels in patients with recurrent infections and healthy controls. While differences in sensitivities between assay systems might explain discrepancies with older studies, they cannot explain the differences between our study and that of Friedman and Kimmel [1982]. One possible explanation might be the use of insufficiently specific antisera against human IgA.…”

Section: Discussionmentioning

confidence: 97%

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Use of enzyme‐labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid

Loon¹,

Logt²,

Heessen³

et al. 1985

Journal of Medical Virology

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A direct enzyme-linked immunosorbent assay (ELISA that used peroxidase-labeled antigen) was developed for detection of IgM and IgA antibody to herpes simplex virus (HSV). The assay uses immuno-affinity-purified antihuman IgM or IgA antibody-coated wells of microtiter plates to separate IgM or IgA from other classes of antibody in serum or cerebrospinal fluid (CSF). The presence of specific IgM or IgA is detected by subsequent, consecutive incubation with peroxidase-labeled antigen and substrate. HSV antigen was purified by sucrose gradient centrifugation and coupled with peroxidase by the periodate method. By examining sucrose-gradient-fractionated sera the assays were shown to be specific for IgM and IgA classes of antibody. None of the sera from patients with Epstein-Barr virus (n = 20), cytomegalovirus (n = 20), or varicella-zoster virus (n = 8) infection or with both rheumatoid factor and IgG antibody to HSV (n = 13) reacted positively. Only one out of 78 sera from healthy persons was positive for IgA antibody to HSV, and none for IgM antibody. All 33 patients with HSV infection developed HSV-IgA, 22 developed HSV-IgM. Of the 11 patients with primary infection, all had IgM antibody in their first sera and six had IgA antibody. The corresponding figures for the 22 patients with recurrent infection were five and nine. Furthermore, HSV-IgA antibody was found in serum and CSF of all five patients with HSV encephalitis in the second week after onset of symptoms, indicating the usefulness of the assay as a noninvasive technique for diagnosing HSV encephalitis.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

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Use of enzyme‐labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid

Loon¹,

Logt²,

Heessen³

et al. 1985

Journal of Medical Virology

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“…The diagnostic value of specific total serum IgA antibodies as marker of virus infections is controversial [Halonen et al, 1979a,b;Nikoskelainen et al, 1979;Friedman and Kimmel, 1982;Sarov and Haikin, 1983;Sikuler et al, 1983;Strand and Hoddevik, 1984;Guglielmino et al, 1989;Vikerfors et al, 1989;Chau et al, 19931. In contrast, different reports have shown the usefulness of virus-specific polymeric IgA as indicator of acute or recent infection [Negro Ponzi et al, 1985;Hashido et al, 1989;Kawano and Minamishima, 19921. Against this background, the presence of heterotypic enteroviral total and polymeric IgA antibodies in serum from patients with acute enterovirus infections were examined and compared with sera from patients with other infections. The presence of heterotypic enteroviral total IgA antibodies in serum samples from blood donors that were collected in different seasons was also investigated and compared.…”

Section: Introductionmentioning

confidence: 99%

Detection of enterovirus‐specific total and polymeric IgA antibodies in serum using a synthetic peptide or heated virion antigen in ELISA

Cello

Svennerholm

1994

Journal of Medical Virology

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The presence of enterovirus-specific total and polymeric IgA antibodies was assessed in serum from different groups of patients and healthy controls by indirect ELISA using heated virions and synthetic peptide, both enteroviral broad reactive antigens. Total IgA antibody response against a synthetic peptide, representing an enterovirus group-common epitope, was detected in 52% of the patients with an acute enterovirus infection and in 12% of the patients with other infections (P = 0.02). We also found a significant difference (P = 0.005) in the prevalence of peptide IgA antibodies between serum samples collected from blood donors during summer (20%), the prevalent season of enterovirus infections, and winter (6%). A polymeric IgA activity against the peptide was detected in only three patients with an enterovirus infection. In contrast, when a heated coxsackie B5 (coxB5) virus antigen was used, the prevalence of total serum IgA antibodies was not significantly different between patients with an acute enterovirus infection and patients with other infections (71% vs. 53% respectively; P = 0.3). Also no difference was found between the two groups of blood donors (47% in summer vs. 51% in winter; P = 0.7). However, the prevalence of serum polymeric IgA antibodies against coxsackie B5 antigen was significantly greater (P = 0.02) in patients with an acute enterovirus infection (57%) than in patients with other infections (18%). These findings suggest that the presence (18%). These findings suggest that the presence of total peptide-IgA or of polymeric coxsackie B5-IgA in serum is a specific marker of acute enterovirus infection. Finally, we show that the total peptide-IgA- and polymeric coxsackie B5-ELISAs may have a diagnostic value for the serodiagnosis of enterovirus infections when they are used in combination with enteroviral IgG-ELISA.

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“…One of the important aspects of genital herpes (GH) is the asymptomatic infection (13). HSV-IgA serum antibody response has been found to correlate well with asymptomatic and overt genital herpes infections (10)(11)(12). In the absence of any past history of HSV infection, a rise in titre might indicate a type-specific antibody response to some occult HSV lesion at a remote site.…”

mentioning

confidence: 99%

HSV‐IgA serum antibodies in cervical intraepithelial neoplasia and invasive cancer patients, and in their spouses: a case control study

Gupta

Jain

Parashari

et al. 1992

APMIS

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Satyanarayana, L. HSV-IgA serum antibodies in cervical intraepithelial neoplasfa and invasive cancer patients, and in their spouses: a case control Class-specific IgG and IgA antibodies to HSV were assayed in women with CIN (76), invasive cancer (52) (histological diagnosis) and age-matched controls (1 19), employing HSV-2-infected HEp-2 cells as antigen during IFA assay. We observed an elevated geometric mean titre (GMT) of serum antibody (IgG five-to eight-fold and IgA four-to five-fold) for the entire spectrum of cervical lesions, as compared to controls. The odds of finding HSV-IgA antibodies were highest with CIN 111 (OR= 22.0), followed by invasive carcinoma, and CIN I & I1 (OR = 9.5 and 5.2), respectively. Furthermore, the investigations with respect to married couples (husbands and wives) who volunteered to participate in this study (33 cases and 47 control group) also indicated relatively high antibody titres and increased frequency of HSV sero positivity amongst husbands of cases as compared to their wives, as well as the control group males and females. The contribution of HSV infection in women and/or their husbands to the risk of developing abnormal cervical lesions was analysed after adjusting for the same in respective counterparts. It was observed that the risk was increased 14-fold with HSV-IgA positivity of women, and that HSV-IgA positivity of husbands (male partners) further increased the risk 16-fold. This preliminary observation shows the importance of serum HSV-IgA antibodies as a risk indicator in cervical precancer and cancer lesions in women without a history of recent genital herpes lesions. The serum HSV-IgA may also be taken as an indicator of "high risk" males.

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Herpes simplex virus-specific serum immunoglobulin a: detection in patients with primary or recurrent herpes infections and in healthy adults

Cited by 31 publications

References 16 publications

Use of enzyme‐labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid

Use of enzyme‐labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid

Detection of enterovirus‐specific total and polymeric IgA antibodies in serum using a synthetic peptide or heated virion antigen in ELISA

HSV‐IgA serum antibodies in cervical intraepithelial neoplasia and invasive cancer patients, and in their spouses: a case control study

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