HSV‐IgA serum antibodies in cervical intraepithelial neoplasia and invasive cancer patients, and in their spouses: a case control study

Abstract

Satyanarayana, L. HSV-IgA serum antibodies in cervical intraepithelial neoplasfa and invasive cancer patients, and in their spouses: a case control Class-specific IgG and IgA antibodies to HSV were assayed in women with CIN (76), invasive cancer (52) (histological diagnosis) and age-matched controls (1 19), employing HSV-2-infected HEp-2 cells as antigen during IFA assay. We observed an elevated geometric mean titre (GMT) of serum antibody (IgG five-to eight-fold and IgA four-to five-fold) for the entire spectrum of cervical lesions, as compared to controls. The odds of finding HSV-IgA antibodies were highest with CIN 111 (OR= 22.0), followed by invasive carcinoma, and CIN I & I1 (OR = 9.5 and 5.2), respectively. Furthermore, the investigations with respect to married couples (husbands and wives) who volunteered to participate in this study (33 cases and 47 control group) also indicated relatively high antibody titres and increased frequency of HSV sero positivity amongst husbands of cases as compared to their wives, as well as the control group males and females. The contribution of HSV infection in women and/or their husbands to the risk of developing abnormal cervical lesions was analysed after adjusting for the same in respective counterparts. It was observed that the risk was increased 14-fold with HSV-IgA positivity of women, and that HSV-IgA positivity of husbands (male partners) further increased the risk 16-fold. This preliminary observation shows the importance of serum HSV-IgA antibodies as a risk indicator in cervical precancer and cancer lesions in women without a history of recent genital herpes lesions. The serum HSV-IgA may also be taken as an indicator of "high risk" males.

Biological behavior and etiology of inflammatory cervical smears

“…Using Ayer's spatula (wooden), cervical scrapes were collected for Pap and Gram stains. From each woman, 5 ml of blood were collected, and serological assays were performed for antibodies to herpes simplex virus (HSV), 9 human immunodefciency virus HSV (I and II) (Veronistika HIV Mixt, Organon Technika, Boxtel, Netherlands), and Trepanoma pallidum (TPHA test kit, Fusirebio, Inc., Tokyo, Japan). Bacterial vaginosis (BV) was diagnosed using any 3 of 5 standardized criteria, 10 i.e., pH Ͼ4.5, amine test positive, foul odor of cervicovaginal secretions, and presence of increased number of clue cells and gardnerella type organisms in the cervical smears.…”

Biological behavior and etiology of inflammatory cervical smears

“…Using Ayer's spatula (wooden), cervical scrapes were collected for Pap and Gram stains. From each woman, 5 ml of blood were collected, and serological assays were performed for antibodies to herpes simplex virus (HSV), 9 human immunodefciency virus HSV (I and II) (Veronistika HIV Mixt, Organon Technika, Boxtel, Netherlands), and Trepanoma pallidum (TPHA test kit, Fusirebio, Inc., Tokyo, Japan). Bacterial vaginosis (BV) was diagnosed using any 3 of 5 standardized criteria, 10 i.e., pH Ͼ4.5, amine test positive, foul odor of cervicovaginal secretions, and presence of increased number of clue cells and gardnerella type organisms in the cervical smears.…”