Herpes simplex virus-1 (HSV-1) infection in radiation-induced oral mucositis

Nicolatou‐Galitis, Ourania; Athanassiadou, Pauline; Kouloulias, Vassilis; Sotiropoulou-Lontou, Anastasia; Dardoufas, K.; Polychronopoulou, Argy; Gonidi, Maria; Kyprianou, Konstantinos; Kolitsi, Georgia; Skarleas, Christos; Pissakas, George; Papanikolaou, Ioannis S.; Kouvaris, John

doi:10.1007/s00520-005-0006-5

Cited by 49 publications

(64 citation statements)

References 25 publications

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“…This may happen due to inclusion of different tumour types, patients with different protocol treatments for RT and/or chemotherapy, collection of oral rinsing samples performed at distinct moments, and lack of serological data [16,17,26]. …”

Section: Discussionmentioning

confidence: 99%

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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Background: Xerostomia is a very relevant and frequent complication of radiotherapy, causing the irradiated oral mucosa to be affected by bacterial, fungal and viral infections. Objective: The objective of this study was to evaluate a possible relationship between oral shedding of human herpesviruses and xerostomia in patients with squamous cell carcinoma of head and neck submitted to radio/chemotherapy. Methods: In this study, oral rinse samples were collected weekly from 20 patients during radiotherapy. The samples were submitted to PCR and enzymatic digestion for detection of human herpesviruses. Xerostomia was evaluated according to the Seminars in Radiation Oncology criteria. Results: There was a higher frequency of grade 1 xerostomia (51.4%), observed first in the 1st week of radiotherapy. In the 4th week of radiotherapy, all patients presented some degree of xerostomia. Analysis of herpesviruses showed oral shedding of EBV, HHV-6 and HHV-7 in all weeks. Considering all the periods, the highest frequency was in patients with EBV excretion (55.0%), which was significantly higher than that of other viruses. Conclusion: We observed that oral shedding of herpesviruses was not affected by xerostomia as there was a progression in their excretion, even with the evolution of xerostomia. This suggested that there is a local replication in the oral cavity that is not completely dependent of salivary excretion.

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Section: Discussionmentioning

confidence: 99%

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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“…Therefore, introduction of antifungal prevention in the form of 100 mg of fluconazole daily may significantly reduce occurrence of pathological changes (9,10) . Proper function of bone marrow and improvement in blood morphology parameters lead to regression of changes in the oral mucosa (11) .…”

Section: Discussionmentioning

confidence: 99%

Oral Mucositis in Children Suffering from Acute Lymphoblastic Leukaemia”

Alani¹,

Hasan²,

Hameed³

2014

Iraqi dent. j.

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Background: Oral mucositis is the most commonly reported side effect observed in neoplastic patients treated with chemotherapy and radiotherapy of the head and neck region as well as in patients who have received a haematopoietic stem cell transplant. The aim of the study was to assess the oral mucosa status in children with acute lymphoblastic leukaemia (ALL) during antineoplastic therapy. Material and methods:The clinical examination included 127 children aged 5-15 with ALL. The clinical examination was conducted using the dental diagnostic instrument. The condition of the oral mucosa was determined using the WHO scale for oral mucositis. Results: In the first period of antineoplastic therapy the pathological lesions of the oral mucosa of the mucositis type were observed among the examined patients. The lesions had various levels of intensity. Pain was found to be the primary symptom of oral mucositis. In this study the following were observed: local erythema of the oral mucosa in 10%, ulcerative lesions in 5%.The remaining 85% patient who could not eat or drink because of pain and soreness. Conclusion: Local treatment of oral mucositis with polyantibiotic-antifungal mixture, supporting antifungal systemic treatment, and improving the overall peripheral blood conditions in children suffering from acute lymphoblastic leukaemia improve the condition of the oral mucosa.

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“…Assim como, também é conhecida, a relação Uma grande dificuldade na comparação de estudos em mucosite oral induzida por radioterapia de cabeça e pescoço e sua possível associação com o HSV-1 é a falta de padronização da amostra, quer seja na inclusão de pacientes com diferentes tipos de tumor, diferentes esquemas de radioterapia e/ou quimioterapia, coletas de saliva realizadas em momentos únicos na maioria das vezes e falta de dados sorológicos (Nicolatou-Galitis et al, 2001;Nicolatou-Galitis et al, 2006;Correia et al, 2015). No presente estudo, todos os pacientes incluídos na amostra foram diagnosticados com carcinoma epidermoide (boca ou orofaringe), como proposta de tratamento foram submetidos à radioterapia, com dose total de 60 Gy, associado à quimioterapia (cisplatina 100mg/m2), preparo odontológico prévio e laserterapia.…”

Section: Discussionunclassified

“…Entretanto, não existem estudos que identifiquem a importância da família Herpesviridae como um todo na manifestação da mucosite oral decorrente da radioterapia, ou mesmo de sua associação com a quimioterapia (Redding et al, 1990;Nicolatou-Galitis et al, 2001;Epstein et al, 2002;Trotti et al, 2003;Nicolatou-Galitis et al, 2006;Correia et al, 2015).…”

Section: Revisão Da Literaturaunclassified

Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço

Palmieri¹

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Palmieri M. Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço. Dissertação apresentada à Faculdade de Odontologia da Universidade de São Paulo, para obter o título de Mestre, pelo Programa de Pós-Graduação em Ciências Odontológicas. A radioterapia para tratamento das neoplasias malignas em região de cabeça e pescoço é acompanhada de diversas complicações, decorrentes do comprometimento dos tecidos radiossensíveis localizados próximos ao tumor.Entre essas complicações a mucosite é a que merece maior destaque. A mucosite é uma reação tóxica inflamatória da mucosa oral causada pelo tratamento citorredutivo induzido pela radioterapia (RT) ou pela quimioterapia (QT). Ela manifesta-se com sinais de edema, eritema, úlcera e formação pseudomembrana, resultando em sintomas de ardência, que pode progredir para dor intensa e consequente prejuízo na alimentação e comunicação verbal.Infecções bacterianas, fúngicas ou virais podem acometer a mucosa bucal irradiada e exacerbar a manifestação da mucosite oral por meio da ativação de fatores de transcrição da resposta inflamatória. Existem poucos dados na literatura sobre a participação dos herpesvirus humanos na mucosite oral induzida pela radioterapia. A proposta desse trabalho foi avaliar a excreção oral dos herpesvirus humanos (HSV-1, HSV-2, EBV, CMV, VZV, HHV6, HHV7 e HHV8) e sua possível associação com o desenvolvimento e agravamento da mucosite oral, em pacientes diagnosticados com carcinoma epidermoide (CEC)de boca e orofaringe, submetidos à radioterapia associado à quimioterapia.Nesse estudo foram analisadas 158 amostras de lavado bucal, de 20 pacientes, submetidos à radioterapia para CEC em região de cabeça e pescoço, coletadas semanalmente, durante todo o tratamento. Foi realizada a extração do DNA dessas amostras e em seguida sua amplificação através da PCR utilizando dois conjuntos de primers: HSVP1/P2 para os subtipos HSV-1, HSV-2, EBV, CMV e HHV-8 e o VZVP1/P2 para os subtipos VZV, HHV-6 e HHV-7. As amostras positivas foram submetidas à digestão enzimática com enzimas de restrição BamHI e BstUI para determinação específica de cada um dos oito herpesvirus. Foi também avaliada clinicamente, a mucosite oral, em cada uma das coletas, seguindo os critérios da OMS e NCIC. As análises da amostra mostraram a excreção do EBV, HHV-6 e HHV-7, em todas as semanas de tratamento radioterápico, enquanto que a excreção do HSV1 não pode ser observada no momento da triagem. Considerando-se todos os períodos em conjunto (Triagem, semanas On our study, we analyzed 158 oral rinsing samples, collected weekly, from 20 patients during the whole radiotherapy treatment for squamous cell carcinoma in head and neck. From these samples, we extracted the DNA and afterwards we amplified them with PCR using two sets of primers:HSVP1/P2 for the subtypes HSV-1, HSV-2, EBV, CMV, and HHV-8 and VZVP1/P2 for the subtypes VZV, HHV 6, and HHV-7. The positive samples were subjected to enzymatic digestion with Ba...

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Herpes simplex virus-1 (HSV-1) infection in radiation-induced oral mucositis

Cited by 49 publications

References 25 publications

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Oral Mucositis in Children Suffering from Acute Lymphoblastic Leukaemia”

Detecção dos herpesvirus humanos na mucosa oral de pacientes irradiados para tratamento de carcinoma epidermoide em região de cabeça e pescoço

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