Heroin Abuse and/or HIV Infection Dysregulate Plasma Exosomal miRNAs

Wang, Xu; Lee, Sun; Zhou, Yu; Su, Qi-jian; Li, Jieliang; Li, Ye; Liu, Man‐Qing; Wang, Zhou; Ho, Wen‐Zhe

doi:10.1007/s11481-019-09892-9

Cited by 15 publications

(12 citation statements)

References 61 publications

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“…However, whether circulating miRNAs can be biomarkers for heroin addiction is not well-studied, and only a few preliminary studies available. Heroin use can upregulate a panel of plasma exosomal miRNAs associated with neuroinflammation and neurotoxicity ( 32 ). And recent studies have reported peripheral miRNAs, including miR-486-5p, miR-206, and let-7b-5p were dysregulated in patients with heroin use disorder ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Liu

Feng

et al. 2021

Front. Psychiatry

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Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomarkers for diagnosing brain diseases such as Alzheimer's disease, multiple sclerosis, schizophrenia, and bipolar disorder. However, studies on circulating miRNAs for the diagnosis of heroin use disorders are rarely reported. In this study, we investigated the differential expression of plasma miRNAs in 57 heroin-dependent patients. Based on literature research and microarray analysis, two candidate miRNAs, miR-320a and let-7b-5p, were selected and analyzed by quantitative real-time RT-PCR. The results showed miR-320a and let-7b were significantly upregulated in plasma of the heroin-dependent patients compared to that in healthy controls. The area under curves (AUCs) of receiver operating characteristic (ROC) curves of miR-320a and let-7b-5p were 0.748 and 0.758, respectively. The sensitivities of miR-320a and let-7b-5p were 71.9 and 70.2%, while the specificities of miR-320a and let-7b-5p were 76.1 and 78.3%, respectively. The combination of these two miRNAs predicted heron dependence with an AUC of 0.782 (95% CI 0.687–0.876), with 73.7% sensitivity and 82.6% specificity. Our findings suggest a potential use for circulating miRNAs as biomarkers for the diagnosis of heroin abuse.

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Section: Discussionmentioning

confidence: 99%

Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Liu

Feng

et al. 2021

Front. Psychiatry

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“…Similarly, a 2019 study investigated the effects of HIV infection and heroin use on inflammation-associated EV miRNA [173]. This study found that HIV-infected heroin users had significantly upregulated levels of miR-146a, miR-126, miR-21, and miR-let-7a, all of which are implicated in neuroinflammation.…”

Section: Evs Substance Abuse and Hivmentioning

confidence: 97%

Role of Extracellular Vesicles in Substance Abuse and HIV-Related Neurological Pathologies

Odegaard

Chand

Wheeler

et al. 2020

IJMS

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Extracellular vesicles (EVs) are a broad, heterogeneous class of membranous lipid-bilayer vesicles that facilitate intercellular communication throughout the body. As important carriers of various types of cargo, including proteins, lipids, DNA fragments, and a variety of small noncoding RNAs, including miRNAs, mRNAs, and siRNAs, EVs may play an important role in the development of addiction and other neurological pathologies, particularly those related to HIV. In this review, we summarize the findings of EV studies in the context of methamphetamine (METH), cocaine, nicotine, opioid, and alcohol use disorders, highlighting important EV cargoes that may contribute to addiction. Additionally, as HIV and substance abuse are often comorbid, we discuss the potential role of EVs in the intersection of substance abuse and HIV. Taken together, the studies presented in this comprehensive review shed light on the potential role of EVs in the exacerbation of substance use and HIV. As a subject of growing interest, EVs may continue to provide information about mechanisms and pathogenesis in substance use disorders and CNS pathologies, perhaps allowing for exploration into potential therapeutic options.

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“…Studies have shown that alterations in circulating miRNA profiles are strongly associated with disease progression as well as the regulation of immune activation and inflammation 31 , consequently, they can be used as biomarkers for cancer and infectious diseases 32 , 33 . For example, the level of plasma exosomal miRNAs that have been identified from HIV infection and/or heroin abuse can be used as biomarkers in immune regulation and neuroinflammation 34 . Furthermore, miRNAs and exosomes have been proposed as new diagnostic biomarkers for patients with anxiety 35 and depression 36 .…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have suggested that miRNAs played pivotal roles in the development of depression and anxiety, and have emerged as potential therapeutic targets and tools for use in the diagnosis and treatment responses of patients with depression and anxiety 38 . However, relatively few studies have focused on the role of circulating exosomal miRNAs in SUD patients 34 , while other two researches were performed on rats 39 , 40 . None of them were conducted in SUD patients with anxiety and depression.…”

Section: Introductionmentioning

confidence: 99%

Prognostic plasma exosomal microRNA biomarkers in patients with substance use disorders presenting comorbid with anxiety and depression

Chen

Zou

Dai

et al. 2021

Sci Rep

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Psychiatric disorders such as anxiety and depression precipitated by substance use occurred during both use and withdrawal. Exosomes play significant roles in biological functions and regulate numerous physiological and pathological processes in various diseases, in particular substance use disorders (SUDs) and other psychiatric disorders. To better understand the role of exosomal miRNAs in the pathology of symptoms of anxiety and depression in patients with SUDs, we first isolated circulating exosomes from heroin-dependent patients (HDPs) and methamphetamine-dependent patients (MDPs) and identified exosomal miRNAs that were differentially expressed between patients and healthy controls (HCs). Furthermore, the correlations between exosomal DE-miRNAs and symptoms of anxiety and depression which were measured using Hamilton-Anxiety (HAM-A)/Hamilton-Depression (HAM-D) Rating Scales in the participants. Notably, the expression level of exosomal hsa-miR-16-5p, hsa-miR-129-5p, hsa-miR-363-3p, and hsa-miR-92a-3p showed significantly negative correlations with HAM-A scores in both HDPs and MDPs. But all of the 4 DE-miRNAs lost significant correlations with HAM-D scores in HDPs. Functional annotation analyses showed that the target genes of the DE-miRNAs were mainly enriched for “synapse”, “cell adhesion”, “focal adhesion” and “MHC class II protein complex”. Our study suggests that a set of circulating exosomal miRNAs were associated with anxiety and depression in SUD patients and may have clinical utility as diagnostic and prognostic biomarkers.

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Heroin Abuse and/or HIV Infection Dysregulate Plasma Exosomal miRNAs

Cited by 15 publications

References 61 publications

Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Role of Extracellular Vesicles in Substance Abuse and HIV-Related Neurological Pathologies

Prognostic plasma exosomal microRNA biomarkers in patients with substance use disorders presenting comorbid with anxiety and depression

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