Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Liu, Haixiong; Xu, Wenjin; Feng, Jiying; Ma, Hong; Xie, Xiaohu; Zhuang, Dingding; Shen, Wenwen; Liu, Huifen; Zhou, Wenhua

doi:10.3389/fpsyt.2021.679206

Cited by 12 publications

(10 citation statements)

References 46 publications

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“…Based on the above studies, we speculated that the dysfunction of miR-320 in plasma and exosomes could reflect the corresponding dysfunction in the brain caused by MA addiction. Previously, our group have found that the levels of miR-181a, miR-320a, and let-7b-5p in circulating plasma significantly increased in heroin-dependent patients, and demonstrated that these miRNAs can distinguish heroin addicts from healthy controls (22,33). Tobacco affects the central and circulating expression of miRNAs and is prevalent in drug-addicted patients (21,34).…”

Section: Discussionmentioning

confidence: 98%

Circulating plasma and exosome levels of the miR-320 family as a non-invasive biomarker for methamphetamine use disorder

Hong

Zhou

et al. 2023

Front. Psychiatry

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The neurobiological mechanism underlying methamphetamine (MA) use disorder was still unclear, and no specific biomarker exists for clinical diagnosis of this disorder. Recent studies have demonstrated that microRNAs (miRNAs) are involved in the pathological process of MA addiction. The purpose of this study was to identify novel miRNAs for the diagnosis biomarkers of MA user disorder. First, members of the miR-320 family, including miR-320a-3p, miR-320b, and miR-320c, were screened and analyzed in the circulating plasma and exosomes by microarray and sequencing. Secondly, plasma miR-320 was quantified by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) in eighty-two MA patients and fifty age-gender-matched healthy controls. Meanwhile, we also analyzed exosomal miR-320 expression in thirty-nine MA patients and twenty-one age-matched healthy controls. Furthermore, the diagnostic power was evaluated using the area under the curve (AUC) of the receiver operating characteristic (ROC) curve. The expression of miR-320 significantly increased in plasma and exosomes of MA patients compared with healthy controls. The AUC of the ROC curves of miR-320 in plasma and exosomes of MA patients were 0.751 and 0.962, respectively. And the sensitivities of miR-320 were 0.900 and 0.846, respectively, whereas the specificities of miR-320 were 0.537 and 0.952, respectively, in plasma and exosomes in MA patients. And the increased plasma miR-320 was positively correlated with cigarette smoking, age of onset, and daily use of MA in MA patients. Finally, cardiovascular disease, synaptic plasticity, and neuroinflammation were predicted to be the target pathways related to miR-320. Taken together, our findings indicated that plasma and exosomal miR-320 might be used as a potential blood-based biomarker for diagnosing MA use disorder.

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Section: Discussionmentioning

confidence: 98%

Circulating plasma and exosome levels of the miR-320 family as a non-invasive biomarker for methamphetamine use disorder

Hong

Zhou

et al. 2023

Front. Psychiatry

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“…Also, Dakterzada aimed to find miRNAs in plasma from AD participants, identifying a BACE-1 related panel of biomarkers different from the miRNAs in the present work 33 . It could be due to the use of a different identification technique based on microarrays analysis 34 . The different methodologies employed could affect the miRNAs selection, so it should be taken into account in comparisons with other studies 33 .…”

Section: Discussionmentioning

confidence: 99%

Plasma microRNAs as potential biomarkers in early Alzheimer disease expression

Peña-Bautista

Tarazona-Sánchez

Braza-Boïls

et al. 2022

Sci Rep

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The microRNAs (miRNAs) are potential biomarkers for complex pathologies due to their involvement in the regulation of several pathways. Alzheimer Disease (AD) requires new biomarkers in minimally invasive samples that allow an early diagnosis. The aim of this work is to study miRNAS as potential AD biomarkers and their role in the pathology development. In this study, participants (n = 46) were classified into mild cognitive impairment due to AD (MCI-AD, n = 19), preclinical AD (n = 8) and healthy elderly controls (n = 19), according to CSF biomarkers levels (amyloid β42, total tau, phosphorylated tau) and neuropsychological assessment. Then, plasma miRNAomic expression profiles were analysed by Next Generation Sequencing. Finally, the selected miRNAs were validated by quantitative PCR (q-PCR). A panel of 11 miRNAs was selected from omics expression analysis, and 8 of them were validated by q-PCR. Individually, they did not show statistically significant differences among participant groups. However, a multivariate model including these 8 miRNAs revealed a potential association with AD for three of them. Specifically, relatively lower expression levels of miR-92a-3p and miR-486-5p are observed in AD patients, and relatively higher levels of miR-29a-3p are observed in AD patients. These biomarkers could be involved in the regulation of pathways such as synaptic transmission, structural functions, cell signalling and metabolism or transcription regulation. Some plasma miRNAs (miRNA-92a-3p, miRNA-486-5p, miRNA-29a-3p) are slightly dysregulated in AD, being potential biomarkers of the pathology. However, more studies with a large sample size should be carried out to verify these results, as well as to further investigate the mechanisms of action of these miRNAs.

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“…We identified 26 microRNAs that are predicted to bind at least three heroin-associated circRNAs and an additional 12 microRNAs predicted to bind circGrin2b, circAdcy5, circAnks1a, circSlc24a2 or circUbe2cbp. Of these, let-7b-5p, which is predicted to bind circAdcy5, has been reported to be elevated in both the serum and the plasma of patients with heroin-use disorder [52,53]. Moreover, the putative targets of the heroinassociated circRNA-miRNA network include genes that have been studied extensively in the field of addiction, including Bax, Egr1, Elk1, Mapk3 (Erk1) and Ncam1.…”

Section: Discussionmentioning

confidence: 99%

Heroin Regulates Orbitofrontal Circular RNAs

Floris

Gillespie

Zanda

et al. 2022

IJMS

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The number of drug overdose deaths involving opioids continues to rise in the United States. Many patients with opioid use disorder (OUD) that seek treatment still experience relapse. Perseverant opioid seeking behaviors represent a major challenge to treating OUD and additional therapeutic development will require insight into opioid-induced neurobiological adaptations. In this study, we explored the regulation of a novel class of RNAs, circular RNAs (circRNAs), by the addictive opioid heroin in the rat orbitofrontal cortex (OFC), a brain region that mediates behavioral responses to rewarding stimuli. Microarray analysis identified 76 OFC circRNAs significantly regulated in male rats after heroin self-administration. We evaluated the specificity of these findings by measuring heroin-associated circRNA expression in female rats after heroin self-administration and in rats that self-administered sucrose. We identify circGrin2b, circUbe2cp, circAnks1a, circAdcy5 and circSlc24A2 as heroin-responsive circRNAs in the OFC. Linear mRNA levels of heroin-associated circRNAs were unchanged except for Grin2b and Adcy5. An integrated bioinformatics analysis of regulated circRNAs identified microRNAs predicted to bind heroin-associated circRNAs and downstream targets of circRNA: microRNA sponging. Thus, heroin regulates the expression of OFC RNA splice variants that circularize and may impact cellular processes that contribute to the neurobiological adaptations that arise from chronic heroin exposure.

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Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Cited by 12 publications

References 46 publications

Circulating plasma and exosome levels of the miR-320 family as a non-invasive biomarker for methamphetamine use disorder

Circulating plasma and exosome levels of the miR-320 family as a non-invasive biomarker for methamphetamine use disorder

Plasma microRNAs as potential biomarkers in early Alzheimer disease expression

Heroin Regulates Orbitofrontal Circular RNAs

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