Heritage of Neem–Peepal Tree Resides a Profound Scientific Facts

“…Next-generation sequencing (NGS) technologies have accelerated the generation of draft genome sequences of Moraceae plant species, the genome size of Morus notabilis is 330Mb [9], 333Mb for Ficus carica [10], 436Mb for F. microcarpa , and 370Mb for F. hispida [11]. The genome sequencing of non-model plant species Peepal was first mentioned in The Neem Genome book chapter [3]. A recent study has generated the genomic resource of F. religiosa (332Mb) and F. benghalensis (392Mb).…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the culture is spread across Asia and it has been worshipped. Peepal has several vernacular names, like Pippali, Ashwatha, Arali, and so on; it is frequently found together with the Neem tree near Indian temples [3]. Generally, the Peepal tree has a special significance in communities across India as it is believed to produce oxygen day and night.…”

Section: Introductionmentioning

confidence: 99%

“…The genome sequencing of non-model plant species F. religiosa was first mentioned in The Neem Genome book chapter [3]. A recent study has generated the genomic resource of F. religiosa (332 Mb) and F. benghalensis (392 Mb).…”

Section: Introductionmentioning

confidence: 99%

“…The genome size of Morus notabilis is 330 Mb [9], 333 Mb in Ficus carica [10], 436 Mb in F. microcarpa and 370 Mb in F. hispida [11]. The genome sequencing of non-model plant species F. religiosa was first mentioned in The Neem Genome book chapter [3]. A recent study has generated the genomic resource of F. religiosa (332 Mb) and F. benghalensis (392 Mb).…”

Section: Introductionmentioning

confidence: 99%

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Genomic and transcriptomic analysis of sacred fig (Ficus religiosa)

Gowda

2022

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Peepal/Bodhi tree (Ficus religiosa L.) is an important long lived keystone ecological species. This plant has been widely used by community in Indian subcontinent in traditional medicine, ayurveda and spirituality. Our study was aimed to generate molecular resources from whole genome and transcriptome sequencing approaches. The whole genome of Peepal tree was sequenced using Illumina and MGISEQ-2000 sequencers. We assembled the draft genome (380-Mb) of Peepal tree and annotated 35,093 protein-coding genes. The 53% of its genome consists of repetitive sequences. To understand the photosynthetic pathways in leaf tissues, we analysed photosynthetically distinct conditions: bright sunny day and dark night. The RNA-seq analysis supported the expression of 26,691 genes. The gene expression analysis of day and night period revealed the molecular switches between the Calvin-Benson (C3) cycle, C4-Dicarboxylic cycle and Crassulacean acid metabolism (CAM) cycle for carbon fixation in leaves of Peepal tree.

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Genomic and transcriptomic analysis of sacred fig (Ficus religiosa)

2023

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Background Peepal/Bodhi tree (Ficus religiosa L.) is an important, long-lived keystone ecological species. Communities on the Indian subcontinent have extensively employed the plant in Ayurveda, traditional medicine, and spiritual practices. The Peepal tree is often thought to produce oxygen both during the day and at night by Indian folks. The goal of our research was to produce molecular resources using whole-genome and transcriptome sequencing techniques. Results The complete genome of the Peepal tree was sequenced using two next-generation sequencers Illumina HiSeq1000 and MGISEQ-2000. We assembled the draft genome of 406 Mb, using a hybrid assembly workflow. The genome annotation resulted in 35,093 protein-coding genes; 53% of its genome consists of repetitive sequences. To understand the physiological pathways in leaf tissues, we analyzed photosynthetically distinct conditions: bright sunny days and nights. The RNA-seq analysis supported the expression of 26,479 unigenes. The leaf transcriptomic analysis of the diurnal and nocturnal periods revealed the expression of the significant number of genes involved in the carbon-fixation pathway. Conclusions This study presents a draft hybrid genome assembly for F. religiosa and its functional annotated genes. The genomic and transcriptomic data-derived pathways have been analyzed for future studies on the Peepal tree.

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Heritage of Neem–Peepal Tree Resides a Profound Scientific Facts

Cited by 4 publications

References 2 publications

Neem in Dermatology

Neem in Dermatology

Genomic and transcriptomic analysis of sacred fig (Ficus religiosa)

Genomic and transcriptomic analysis of sacred fig (Ficus religiosa)

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