Hericium erinaceus enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells

Lee, Jong Seok; Hong, Eock Kee

doi:10.1016/j.canlet.2010.05.006

Cited by 91 publications

(45 citation statements)

References 43 publications

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“…The cells in the early stages of apoptosis were annexin V-positive and PI-negative; however, the cells in the late stages of apoptosis were both annexin V-and PI-positive. The apoptotic index (%) was calculated as the sum of early and late apoptotic cells divided for the total number of events (29).…”

Section: Mushroom Extracts and The Chemotherapeutic Drugmentioning

confidence: 99%

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Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

2011

Int J Oncol

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Abstract. It has been demonstrated that the Agaricus blazei Murill (ABM) mushroom, which primarily consists of polysaccharides, possesses anti-tumor activities. However, the mechanisms by which ABM inhibits human hepatocellular carcinoma growth remain unknown. Our study demonstrates that ABM acts as an enhancer to sensitize doxorubicin (Dox)-mediated apoptotic signaling, and this sensitization can be achieved by enhancing intracellular Dox accumulation via the inhibition of NFκB activity. These findings suggest that ABM, when combined with low doses of Dox, has the potential to provide more efficient therapeutic effects against drug-resistant human hepatocellular carcinoma.

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Section: Mushroom Extracts and The Chemotherapeutic Drugmentioning

confidence: 99%

“…The membranes were washed with TBS-T after each antibody binding reaction. Detection of each protein was performed using an enhanced chemiluminescence kit (Millipore Co., Billerica, MA, USA) (29).…”

Section: Mushroom Extracts and The Chemotherapeutic Drugmentioning

confidence: 99%

Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

2011

Int J Oncol

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“…Intraperitoneal administration of HE extracts to tumor-bearing mice for a 2-week period significantly reduced tumor size through the induction of NK cell activity, activation of macrophages, and inhibition of angiogenesis (11). Polysaccharides from HE also enhanced apoptosis induced by doxorubicin, a chemotherapeutic drug, in human hepatocellular carcinoma cells (13). Compounds isolated from the fruiting body of HE also reduced proliferation of HL-60 leukemia cells through induction of apoptosis (14).…”

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confidence: 99%

Hericium erinaceus extracts alter behavioral rhythm in mice

et al. 2016

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Hericium erinaceus (HE), an edible mushroom, has been used as a herbal medicine in several Asian countries since ancient times. HE has potential as a medicine for the treatment and prevention of dementia, a disorder closely linked with circadian rhythm. This study investigated the effects of the intake of HE extracts on behavioral rhythm, photosensitivity of the circadian clock, and clock gene mRNA expression in the suprachiasmatic nucleus (SCN), a central clock, in mice. Although the HE ethanol extract only affected the offset time of activity, the HE water extract advanced the sleep-wake cycle without affecting the free-running period, photosensitivity, or the clock gene mRNA expression in SCN. In addition, both extracts decreased wakefulness around end of active phase. The findings of the present study suggest that HE may serve as a functional food in the prevention and treatment of Alzheimer's disease and delayed sleep phase syndrome.Hericium erinaceus (HE), an edible mushroom, has been used as a herbal medicine in several Asian countries since ancient times. HE has been reported to possess antitumor properties. Intraperitoneal administration of HE extracts to tumor-bearing mice for a 2-week period significantly reduced tumor size through the induction of NK cell activity, activation of macrophages, and inhibition of angiogenesis (11). Polysaccharides from HE also enhanced apoptosis induced by doxorubicin, a chemotherapeutic drug, in human hepatocellular carcinoma cells (13). Compounds isolated from the fruiting body of HE also reduced proliferation of HL-60 leukemia cells through induction of apoptosis (14). In addition, HE has been reported to have beneficial effects on the central nervous system, with HE intake found to reduce depression and anxiety in women (21). Hericenones isolated from the fruiting body and erinacines isolated from the mycelium of HE promoted nerve growth factor (NGF) biosynthesis in rodent astrocytes cultures (15). HE also prevented cognitive deficits induced by intracerebroventricular administration of amyloid β (25-35) peptide in mice (18). HE has been found to be effective in improving mild cognitive impairment in humans, which might be attributable to NGF synthesis promoting effect by hericenones (16). Repeated treatment with HE had neuroprotective effects in mice that were subjected to middle cerebral artery occlusion, through promotion of NGF synthesis (3). These studies suggest that HE has potential as a medicine for treatment and prevention of dementia. Dementia is closely linked with circadian clock, an internal clock that generates approximately 24 hours rhythm called as the circadian rhythm, and patients with dementia show functional circadian clock impairments (26). Although some studies have demonstrated that HE might be effective in the treatment and prevention of dementia, no previous studies have

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“…Either the H. erinaceus mycelium (erinacines A-I) or its fruit bodies (hericenones C-H) are the source of many bioactive extracts with valuable biological properties [3]. This study has identified that H. erinaceus has a number of therapeutic properties, such as antioxidant activity [1], hypolipidemic activity [4], hemagglutinating activity [5], antimicrobial activity [6], antiaging activity [7], and anticancer activity [8, 9]. Erinacine A (Fig.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Proteomic Analysis of Erinacine A’s Inhibition of Gastric Cancer Cell Viability and Invasiveness

Kuo

Lee

et al. 2017

Cell Physiol Biochem

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Background / Aims: Erinacine A, isolated from the ethanol extract of the Hericium erinaceus mycelium, has been demonstrated as a new alternative anticancer medicine. Drawing upon current research, this study presents an investigation of the molecular mechanism of erinacine A inhibition associated with gastric cancer cell growth. Methods: Cell viability was determined by Annexin V–FITC/propidium iodide staining and migration using a Boyden chamber assay to determine the effects of erinacine A treatment on the proliferation capacity and invasiveness of gastric cancer cells. A proteomic assay provided information that was used to identify the differentially-expressed proteins following erinacine A treatment, as well as the mechanism of its targets in the apoptotic induction of erinacine A. Results: Our results demonstrate that erinacine A treatment of TSGH 9201 cells increased cytotoxicity and the generation of reactive oxygen species (ROS), as well as decreased the invasiveness. Treatment of TSGH 9201 cells with erinacine A resulted in the activation of caspases and the expression of TRAIL. Erinacine A induction of apoptosis was accompanied by sustained phosphorylation of FAK/AKT/p70S6K and the PAK1 pathways, as well as the generation of ROS. Furthermore, the induction of apoptosis and anti-invasion properties by erinacine A could involve the differential expression of the 14-3-3 sigma protein (1433S) and microtubule-associated tumor suppressor candidate 2 (MTUS2), with the activation of the FAK/AKT/p70S6K and PAK1 signaling pathways. Conclusions: These results lead us to speculate that erinacine A may generate an apoptotic cascade in TSGH 9201 cells by activating the FAK/AKT/p70S6K/PAK1 pathway and upregulating proteins 1433S and MTUS2, providing a new mechanism underlying the anti-cancer effects of erinacine A in human gastric cancer cells.

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Hericium erinaceus enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells

Cited by 91 publications

References 43 publications

Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

<i><b>Hericium erinaceus</b></i><b> extracts alter behavioral rhythm in </b><b>mice </b>

A Comparative Proteomic Analysis of Erinacine A’s Inhibition of Gastric Cancer Cell Viability and Invasiveness

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