Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

Li, Hong

doi:10.3892/ijo.2010.852

Cited by 8 publications

(7 citation statements)

References 34 publications

(45 reference statements)

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“…NF-κB regulates cell survival and its activation contributes to drug resistance via inhibiting the pro-apoptotic effects of chemotherapy. The studies demonstrated that Agaricus blazei and Hericium erinaceus could sensitize doxorubicin-mediated apoptosis through inhibiting NF-κB activation [ 146 , 147 ]. It was reported that the PI3K/AKT pathway contributed to drug resistance of cancer cells, and inactivation of the pathway by Pleurotus pulmonarius significantly enhanced the sensitivity of HCC cells to cisplatin [ 134 ].…”

Section: Effects Of Combination Of Dietary Natural Products With Amentioning

confidence: 99%

Dietary Natural Products for Prevention and Treatment of Liver Cancer

et al. 2016

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Liver cancer is the most common malignancy of the digestive system with high death rate. Accumulating evidences suggests that many dietary natural products are potential sources for prevention and treatment of liver cancer, such as grapes, black currant, plum, pomegranate, cruciferous vegetables, French beans, tomatoes, asparagus, garlic, turmeric, ginger, soy, rice bran, and some edible macro-fungi. These dietary natural products and their active components could affect the development and progression of liver cancer in various ways, such as inhibiting tumor cell growth and metastasis, protecting against liver carcinogens, immunomodulating and enhancing effects of chemotherapeutic drugs. This review summarizes the potential prevention and treatment activities of dietary natural products and their major bioactive constituents on liver cancer, and discusses possible mechanisms of action.

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Section: Effects Of Combination Of Dietary Natural Products With Amentioning

confidence: 99%

Dietary Natural Products for Prevention and Treatment of Liver Cancer

et al. 2016

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“…It is one of the most important edible and culinary medicinal species. [7778] It is cultivated commercially for the health food market and is shown to possess a range of medicinal properties against diseases like cancer[7980] and chronic hepatitis. [81] Preclinical studies have shown it to possess anti-inflammatory effects against a range of ulcerogens.…”

Section: Dietary Agents With Anti-ibd Effectsmentioning

confidence: 99%

Dietary Agents and Phytochemicals in the Prevention and Treatment of Experimental Ulcerative Colitis

Saxena

Kaur

Hegde

et al. 2014

Journal of Traditional and Complementary Medicine

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Inflammatory bowel diseases (IBDs), consisting mainly of ulcerative colitis (UC) and Crohn's disease (CD), are important immune-mediated diseases of the gastrointestinal tract. The etiology of the disease includes environmental and genetic factors. Its management presents a constant challenge for gastroenterologists and conventional surgeon. 5-Amninosalicylates, antibiotics, steroids, and immune modulators have been used to reduce the symptoms and for maintenance of remission. Unfortunately, long-term usage of these agents has been found to lead to severe toxicities, which are deterrent to the users. Pre-clinical studies carried out in the recent past have shown that certain dietary agents, spices, oils, and dietary phytochemicals that are consumed regularly possess beneficial effects in preventing/ameliorating UC. For the first time, this review addresses the use of these dietary agents and spices in the treatment and prevention of IBD and also emphasizes on the mechanisms responsible for their effects.

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“…Moreover, DOX causes early activation of p53 in tumor cells. Upon activation of the p53 pathway, the expression of the apoptotic regulatory protein, B-cell lymphoma 2 (Bcl-2) is decreased, whereas the downstream caspases, caspase-9, and caspase-3 are activated, leading to apoptosis [ 36 ]. However, the clinical use of DOX is limited by its severe side effects and the associated developed drug resistance, thus, single-agent chemotherapy is no longer appropriate for treating human tumors.…”

Section: Introductionmentioning

confidence: 99%

Manuka honey enhanced sensitivity of HepG2, hepatocellular carcinoma cells, for Doxorubicin and induced apoptosis through inhibition of Wnt/β-catenin and ERK1/2

et al. 2021

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Background Recently, there is increasing awareness focused on the identification of naturally occurring anticancer agents derived from natural products. Manuka honey (MH) has been recognized for its biological properties as antimicrobial, antioxidant, and anticancer properties. However, its antiproliferative mechanism in hepatocellular carcinoma is not investigated. The current study focused mainly on investigating the molecular mechanism and synergistic effect of anticancer properties of MH on Doxorubicin (DOX)-mediated apoptotic cell death, using two different p53 statuses (HepG2 and Hep3B) and one non-tumorigenic immortalized liver cell line. Results MH treatment showed a proliferative inhibitory effect on tested cells in a dose-dependent manner with IC50 concentration of (6.92 ± 0.005%) and (18.62 ± 0.07%) for HepG2 and Hep3B cells, respectively, and induced dramatic morphological changes of Hep-G2 cells, which considered as characteristics feature of apoptosis induction after 48 h of treatment. Our results showed that MH or combined treatments induced higher cytotoxicity in p53-wild type, HepG2, than in p53-null, Hep3B, cells. Cytotoxicity was not observed in normal liver cells. Furthermore, the synergistic effect of MH and Dox on apoptosis was evidenced by increased annexin-V-positive cells and Sub-G1 cells in both tested cell lines with a significant increase in the percentage of Hep-G2 cells at late apoptosis as confirmed by the flow cytometric analysis. Consistently, the proteolytic activities of caspase-3 and the degradation of poly (ADP-ribose) polymerase were also higher in the combined treatment which in turn accompanied by significant inhibitory effects of pERK1/2, mTOR, S6K, oncogenic β-catenin, and cyclin D1 after 48 h. In contrast, the MH or combined treatment-induced apoptosis was accompanied by significantly upregulated expression of proapoptotic Bax protein and downregulated expression of anti-apoptotic Bcl-2 protein after 48 h. Conclusions Our data showed a synergistic inhibitory effect of MH on DOX-mediated apoptotic cell death in HCC cells. To our knowledge, the present study provides the first report on the anticancer activity of MH and its combined treatment with DOX on HCC cell lines, introducing MH as a promising natural and nontoxic anticancer compound.

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Agaricus blazei Murill enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells by NFÎºB-mediated increase of intracellular doxorubicin accumulation

Cited by 8 publications

References 34 publications

Dietary Natural Products for Prevention and Treatment of Liver Cancer

Dietary Natural Products for Prevention and Treatment of Liver Cancer

Dietary Agents and Phytochemicals in the Prevention and Treatment of Experimental Ulcerative Colitis

Manuka honey enhanced sensitivity of HepG2, hepatocellular carcinoma cells, for Doxorubicin and induced apoptosis through inhibition of Wnt/β-catenin and ERK1/2

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