Heregulin promotes expression and subcellular redistribution of ADP‐ribosylation factor 3

Abstract

To identify genes whose expression is modulated by heregulin-L L1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed di¡erential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protein functioning in vesicular traf¢cking, phospholipase D activation and intracellular transport. HRG treatment increased expression of ARF3 mRNA and protein. Also, HRG triggered a rapid redistribution of ARF3, ¢rst to cell membranes and then to the nuclear compartment, where ARF3 colocalized with acetylated histone H3 in discrete regions. In addition, the ARF3 protein was developmentally regulated in the mammary gland with the highest levels in virgin and post-weaning glands. Together, these ¢ndings suggest for the ¢rst time that stimulation of ARF3 expression, subcellular redistribution and interaction with acetylated histone H3 may play a role in the action of HRG in mammary epithelial cells. ß

Systematic Differences in Signal Emitting and Receiving Revealed by PageRank Analysis of a Human Protein Interactome

“…For other proteins, we may expect that they are distributed differently from the feedback pathway proteins. We did literature search of the bottom 10 proteins in reverse ranking of the 2,249 proteins ( File S8 ) and confirmed, as expected, their nucleus localization for the proteins TNS4 [26], SUPT6H [27], ALX and HSH2D [28], ARF3 [29], and Nek9 (at least a small portion located in the nucleus) [30]. Learned from Table 5 , it is also known that top ranked proteins, regardless of in forward ranking or reverse ranking, have more possibility to be located in the cytosol or in both nucleus and a non-nucleus place than the bottom ranked ones.…”

ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway

“…ADP-ribosylation factor (ARFs) are classified as three classes, including ARF1, ARF2, ARF3, ARF4, ARF5, ARF6 (Li et al, 2002;Kahn et al, 2006). As a key regulator of subcellular compartments' formation, ARFs play an important role in vesicular trafficking and activating phospholipase D (Seo et al, 2020).…”