ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway

Liu, Jiayun; Liu, Shenlin; Wu, Maolin; Deng, Jing; Yao, Xuequan; Liu, Fukun; Wu, Xiaoyu; Wu, Guannan

doi:10.18388/abp.2020_5519

Cited by 6 publications

(4 citation statements)

References 25 publications

(28 reference statements)

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“…This was mirrored in analysis of prostate cancer patients who possessed metastatic disease when the key ARF3-RAB11FIP4 cargo N-cadherin protein levels were low. In breast cancers, ARF3 upregulation was reported as associated with pro-proliferative functions 30 , while in gastric cancer, similar to our findings in prostate cells, ARF3 suppressed proliferative function and in vivo tumourigenesis 31 . Indeed, in our own analysis while ARF3 expression was commonly altered in tumour compared to normal tissue, the directionality of expression change was dependent on tumour type.…”

Section: Discussionsupporting

confidence: 89%

The small GTPase ARF3 controls metastasis and invasion modality by regulating N-cadherin levels

Sandilands

Freckmann

Román-Fernández

et al. 2022

Preprint

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ARF GTPases are central regulators of membrane trafficking that act by controlling local membrane identity and remodelling to facilitate vesicle formation. Unravelling ARF GTPase function is complicated by the overlapping association of ARFs with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and numerous interactors. The extent to which redundancy is a major factor in ARF function or whether individual ARF GTPases make unique contributions to cellular behaviour remains unclear. Through a functional genomic screen of 3-Dimensional (3D) prostate cancer cell behaviour we explore the contribution of all known ARF GTPases, GEFs, GAPs, and a large selection of interactors to collective morphogenesis. This revealed that the ARF3 GTPase regulates the modality of invasion, acting as a switch between leader cell-led chains of invasion or collective sheet movement. Functionally, the ability of ARF3 to control invasion modality is dependent on association and subsequent control of the junctional adhesion molecule N-cadherin. In vivo, ARF3 levels acted as a rheostat for metastasis from intraprostatic tumour transplants and ARF3:N-cadherin expression can be used to identify prostate cancer patients with metastatic, poor-outcome disease. Our analysis defines a unique function for the ARF3 GTPase in controlling how cells collectively organise during invasion and metastasis.

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Section: Discussionsupporting

confidence: 89%

The small GTPase ARF3 controls metastasis and invasion modality by regulating N-cadherin levels

Sandilands

Freckmann

Román-Fernández

et al. 2022

Preprint

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“…In line with our data, elongated spindles result from the depletion of the constituent Golgi proteins, which keep the integrity of the organelle in mouse oocyte 90 . The role of ARF1 in mediating Golgi morphological changes during mitosis 43 , and the function of class I ARF proteins in controlling proliferation have been reported 118 , 119 , including the ARF3 involvement in cell cycle progression and apoptosis in gastric cancer 118 . Our zebrafish data show variably penetrant effects on cell proliferation and death, especially for p.P47S and p.T32N.…”

Section: Discussionmentioning

confidence: 99%

Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish

et al. 2022

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Vesicle biogenesis, trafficking and signaling via Endoplasmic reticulum-Golgi network support essential developmental processes and their disruption lead to neurodevelopmental disorders and neurodegeneration. We report that de novo missense variants in ARF3, encoding a small GTPase regulating Golgi dynamics, cause a developmental disease in humans impairing nervous system and skeletal formation. Microcephaly-associated ARF3 variants affect residues within the guanine nucleotide binding pocket and variably perturb protein stability and GTP/GDP binding. Functional analysis demonstrates variably disruptive consequences of ARF3 variants on Golgi morphology, vesicles assembly and trafficking. Disease modeling in zebrafish validates further the dominant behavior of the mutants and their differential impact on brain and body plan formation, recapitulating the variable disease expression. In-depth in vivo analyses traces back impaired neural precursors’ proliferation and planar cell polarity-dependent cell movements as the earliest detectable effects. Our findings document a key role of ARF3 in Golgi function and demonstrate its pleiotropic impact on development.

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“…Patients with bladder cancer with high levels of ARL14 methylation have a worse prognosis ( 112 ). ARF3, a homolog of ARL14, is said to express itself insufficiently in gastric cancer and may function as a possible biomarker for the prognosis of gastric cancer ( 113 ).…”

Section: Additional Factors As Prognostic Indicators For Lung Cancermentioning

confidence: 99%

Serum cytokine levels and other associated factors as possible immunotherapeutic targets and prognostic indicators for lung cancer

Zhao

Jia²,

Zhang³

2023

Front. Oncol.

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Lung cancer is one of the most prevalent cancer types and the leading cause of cancer-related deaths worldwide. Non-small cell lung cancer (NSCLC) accounts for 80-85% of all cancer incidences. Lung cancer therapy and prognosis largely depend on the disease’s degree at the diagnosis time. Cytokines are soluble polypeptides that contribute to cell-to-cell communication, acting paracrine or autocrine on neighboring or distant cells. Cytokines are essential for developing neoplastic growth, but they are also known to operate as biological inducers following cancer therapy. Early indications are that inflammatory cytokines such as IL-6 and IL-8 play a predictive role in lung cancer. Nevertheless, the biological significance of cytokine levels in lung cancer has not yet been investigated. This review aimed to assess the existing literature on serum cytokine levels and additional factors as potential immunotherapeutic targets and lung cancer prognostic indicators. Changes in serum cytokine levels have been identified as immunological biomarkers for lung cancer and predict the effectiveness of targeted immunotherapy.

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ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway

Cited by 6 publications

References 25 publications

The small GTPase ARF3 controls metastasis and invasion modality by regulating N-cadherin levels

The small GTPase ARF3 controls metastasis and invasion modality by regulating N-cadherin levels

Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish

Serum cytokine levels and other associated factors as possible immunotherapeutic targets and prognostic indicators for lung cancer

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