The objective of this study was to determine the differences between the healthy Holsteın-Friesian, Jersey, Simmental, and Aberdeen Angus breeds Beta-hydroxybutyrate (BHBA) levels at the first three weeks after calving. The blood samples were collected for analysis between 10-20 days postpartum. In this study, 80 animals were used in different regions in total. BHBA was analyzed by Ketosite®( Ketosite®, Stanbio Labrotory Inc., Boerne, TX USA). Milk fat and protein levels were immediately measured by an electronic milk analyser (Milkoscan FT1 FOSS industries, Denmark). Statistical analyses of the study were performed using Sigma Plot 12 ( Systat Software Inc., San Lose, CA). The means of BHBA levels were compared by One Way Anova. As in Holstein cows, blood BHBA levels increased in the early lactation period and also in other breeds. The results of this study presented that serum BHBA levels were significantly higher in Holsteın-Friestein cows than in Simmental and Aberdeen Angus cows.