Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants.

Kirchhof, Gudrun; Eckert, Barbara; Stoffels, Marion; Baldani, José Ivo; Reis, Verônica Massena; Hartmann, Anton

doi:10.1099/00207713-51-1-157

Cited by 156 publications

(82 citation statements)

References 38 publications

(47 reference statements)

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“…Isolates B65, B501, and B512 from wild rice species Oryza barthii, O. officinalis, and O. rufipogon, respectively, were closely related to Herbaspirillum spp. in ␤-Proteobacteria, which are known to be diazotrophic endophytes from sugarcane and other gramineous plants (3,20,23). Conversely, isolates B508-1, B511, and B513 from indica and javanica types of cultivated rice plants (O. sativa cultivars Kasalath and SC41, respectively) were closely related to Ideonella dechloratans (21) in ␤-Proteobacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Utilization of carbon sources such as meso-erythritol and N-acetylglucosamine has been used as criteria for species identification of Herbaspirillum (1,3,20). Isolates B65, B501, and B512 differed from H. seropedicae, H. rubrisubalbicans, and H. frisingense in the utilization of meso-erythritol, N-acetylglucosamine, L-rhamnose, and meso-inositol (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, a mild plant pathogen in sugarcane was reclassified as Herbaspirillum rubrisubalbicans (1). Recently, a new species, Herbaspirillum frisingense, isolated from C 4 fiber plants was proposed (20). We then examined whether diazotrophic isolates B65, B501, and B512 from wild rice could be classified as any of the known species of Herbaspirillum (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Isolates B65, B501, and B512 differed from H. seropedicae, H. rubrisubalbicans, and H. frisingense in the utilization of meso-erythritol, N-acetylglucosamine, L-rhamnose, and meso-inositol (Table 2). Kirchhof et al (20) developed several diagnostic probes of a 16S rRNA-targeted oligonucleotide (18 bp) that are specific for the genus Herbaspirillum and Herbaspirillum species. Complementary sequences of the diagnostic probes were compared with DNA sequences of our 16S rRNA genes, and identity was expressed as percentage similarity in Table 2.…”

Section: Resultsmentioning

confidence: 99%

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Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

El-Beltagy

Nishioka

Satô

et al. 2001

Appl Environ Microbiol

421

302

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Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum, Ideonella, Enterobacter, and Azospirillum. Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis, endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa, no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis, was detected by the acetylene reduction and 15 N 2 gas incorporation assays. Therefore, we conclude that Herbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

El-Beltagy

Nishioka

Satô

et al. 2001

Appl Environ Microbiol

421

302

View full text Add to dashboard Cite

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“…Recently, genome sequence analysis of the latter strain showed that it does not possess nif genes (45). This result suggests that testing of nitrogenase activity, which is not part of the clinical laboratory routine, may help in distinguishing the human isolates from diazotrophic environmental strains such as H. seropedicae, H. rubrisubalbicans, and H. frisingense (21,28,29).…”

mentioning

confidence: 97%

Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

et al. 2015

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e Herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. Here, biochemical tests, matrix-assisted laser deionization-time of flight (MALDI-TOF) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of Herbaspirillum spp. Discrete biochemical differences were observed between human and environmental strains. All strains adhered to HeLa cells at low densities, and cytotoxic effects were discrete, supporting the view that Herbaspirillum bacteria are opportunists with low virulence potential.

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Sustainable Cellulosic Grass Crop Production

Fike¹,

Parrish²,

Fike³

2013

Biofuel Crop Sustainability

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Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants.

Cited by 156 publications

References 38 publications

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

Endophytic Colonization and In Planta Nitrogen Fixation by a Herbaspirillum sp. Isolated from Wild Rice Species

Biochemical Characteristics, Adhesion, and Cytotoxicity of Environmental and Clinical Isolates of Herbaspirillum spp

Sustainable Cellulosic Grass Crop Production

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