Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL‐2, TLR4, and PCNA Expression Levels

Abstract

Aim of the Study. To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action. Materials and Methods. ALF was induced in Wistar rats by administrating D-GalN (900 mg/kg) and lipopolysaccharide (10 μg/kg). After treatment with JDHY granules, the levels of blood alanine aminotransferase, aspartate aminotransferase, total bilirubin, and prothrombin time were determined. Proliferating cell nuclear antigen was detected by immunohistochemistry staining. The expression of interleukin-2 (IL-2) and toll-like receptor 4 (TLR4) was examined by fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results. JDHY treatment dramatically improved liver function and increased survival rates in an ALF model in rats. We observed a decrease in IL-2 and TLR4 expression following treatment with JDHY in liver cells from ALF rats using qRT-PCR and Western blot analysis. Conclusion. We hypothesize that the therapeutic potential of JDHY for treating ALF is due to its modulatory effect on the suppression of inflammation and by promoting hepatocyte regeneration. Our results contribute towards validation of the traditional use of JDHY in the treatment of liver disease.

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

“…The role of GA, SY or GA and SY in hepatic regeneration was investigated by detecting the expression of PCNA mRNA and immunostained antibody. Commonly, upregulation of PCNA can increase the survival rate of cells and directly accelerates the DNA replication and cell proliferation (Paunesku and others ; Domitrović and others ).This study showed that GA and SY combination stimulate hepatic regeneration by overexpression of PCNA, this result confirmed by others (Huang and others ; Wang and others ).…”

ErHuang Formula Improves Renal Fibrosis in Diabetic Nephropathy Rats by Inhibiting CXCL6/JAK/STAT3 Signaling Pathway

“…In this study, the level of IL-6 and TNF-a was lower in EHF group compared to the model group, suggesting that EHF could exert anti-inflammatory effect in the treatment of DN. Moreover, proliferating cell nuclear antigen (PCNA) is closely related to the synthesis of DNA and plays an important role in the initiation of cell proliferation (Summary, 2017). In this manuscript, PCNA protein level was strongly suppressed by EHF, representing the anti-proliferative effect of EHF.…”

<p>The underlying mechanisms of Jie-Du-Hua-Yu granule for protecting rat liver failure</p>

“…Jie-Du-Hua-Yu (JDHY) granule is a traditional Chinese herbal compound, which has been used for over two decades in the treatment of liver diseases. This formula consists of six herbs: Radix paeoniae rubrathe, Artemisia capillaris, Rheum officinale, Radix curcumae, Oldenlandia diffusa, and Acorus gramineus 7,8. Our previous clinical studies suggested that JDHY exhibited protective effect in hepatitis B-related ALF patients and improved liver function 9.…”

“…However, the underlying mechanism was poorly understood. Based on previous reports on the beneficial activities of JDHY,7 we hypothesized that JDHY may attenuate LPS-induced liver injury in animal studies. To clarify its protective mechanism, we analyzed the effects of JDHY on LPS-induced damage both in vitro and in vivo.…”