Herbaceous peony tryptophan decarboxylase confers drought and salt stresses tolerance

Zhao, Daqiu; Zhang, Xiayan; Wang, Rong; Liu, Ding; Sun, Jing; Tao, Jun

doi:10.1016/j.envexpbot.2019.03.013

Cited by 25 publications

(18 citation statements)

References 43 publications

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“…The OpTDC2 activity was measured by calculating the production of tryptamine, as described previously [26‐27]. To avoid tryptophan and PLP form an irreversible Pictet‐Spengler reaction in solution, we first mixed the 1 mM PLP with the OpTDC2 [54.6 pkat (mg protein) –1 ], then 1 mM l ‐tryptophan was added into that solution to start this catalytic reaction.…”

Section: Methodsmentioning

confidence: 99%

Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila

You

Feng

Wang

et al. 2020

Biotech and App Biochem

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Tryptophan decarboxylase (TDC, EC 4.1.1.28) catalyzes tryptophan decarboxylation to form tryptamine through the cofactor pyridoxal‐5′‐phosphate (PLP), a crucial stage in the production of the terpenoid indole alkaloids like camptothecin (CPT). A new gene encoding TDC was identified from the CPT‐producing plant Ophiorrhiza pumila by transcriptome analysis, termed OpTDC2. It contained a 1,536 bp open reading frame that encodes a 511 amino acid protein with a molecular mass of 57.01 kDa and an isoelectric point of 6.39. Multiple sequence alignment and phylogenetic tree analysis showed the closest similarity (85%) with the TDC from Mitragyna speciosa. Moreover, the highest expression of OpTDC2 was observed in the O. pumila root. To achieve high‐efficiency expression of OpTDC2 in Escherichia coli, we fused the TF tag onto the N‐terminal of the OpTDC2. Optimum enzymatic activity was observed at 45 °C, pH 8 and cofactor concentration of 0.1 mM. The catalytic reaction was strongly inhibited by metal ions of Cu2+, Zn2+, and Fe2+. The l‐tryptophan was particularly catalyzed compared with d‐tryptophan. Besides, the Km and kcat of the OpTDC2 were 1.08 mM and 0.78 Sec−1, respectively. The results provided information on new functional OpTDC2 that might be used in synthetic biology for the enhanced biosynthesis of CPT in O. pumila.

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Section: Methodsmentioning

confidence: 99%

Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila

You

Feng

Wang

et al. 2020

Biotech and App Biochem

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“…The tryptophan decarboxylase gene of P. lactiflora (PlTDC) in the melatonin biosynthesis pathway enhanced the tolerance of transgenic tobacco to drought, which was induced for 18 days in the absence of water at 22°C. When the NaCl concentration was 200 mM, there was no difference between transgenic and WT leaf disks, but when the NaCl concentration was >200 mM, the green area of leaf disks of WT lines gradually shrank, especially at 800 mM, where the WT leaf disks all turned brown, in contrast to those of transgenic lines, in which only a few turned brown 177 . When P. lactiflora was treated at a low temperature (4°C), the expression level of the cold resistance gene PlGPAT remained high for 72 h, indicating its role in cold resistance 178 .…”

Section: Abiotic Stress Resistancementioning

confidence: 95%

Germplasm resources and genetic breeding of Paeonia: a systematic review

Yang

Sun

et al. 2020

Hortic Res

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Members of the genus Paeonia, which consists of globally renowned ornamentals and traditional medicinal plants with a rich history spanning over 1500 years, are widely distributed throughout the Northern Hemisphere. Since 1900, over 2200 new horticultural Paeonia cultivars have been created by the discovery and breeding of wild species. However, information pertaining to Paeonia breeding is considerably fragmented, with fundamental gaps in knowledge, creating a bottleneck in effective breeding strategies. This review systematically introduces Paeonia germplasm resources, including wild species and cultivars, summarizes the breeding strategy and results of each Paeonia cultivar group, and focuses on recent progress in the isolation and functional characterization of structural and regulatory genes related to important horticultural traits. Perspectives pertaining to the resource protection and utilization, breeding and industrialization of Paeonia in the future are also briefly discussed.

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“…Biochemical and molecular research contributes to better understanding and possible regulation of developmental traits, from stress tolerance [ 60 , 61 ] to flower color [ 62 , 63 ] and biosynthesis of secondary metabolites [ 64 ]. Currently, the first priority for genetic research should be introduction of disease resistance to new cultivars, and recent reports confirm possibility of this direction [ 65 – 67 ].…”

Section: Future Outlookmentioning

confidence: 99%

Cut peony industry: the first 30 years of research and new horizons

Kamenetsky-Goldstein

2022

Horticulture Research

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Herbaceous peony is an ancient medicinal and ornamental crop, cultivated in China and Japan for thousands of years. Numerous varieties of different colors are popular garden plants in different continents and countries. In recent decades, peony has gained a new reputation as cut flowers. Only in Europe, in 30 years, trade in cut peony stems has increased 50 fold. Today, more than 25 countries produce cut peony flowers, with primary markets in Europe, Asia and the USA. This short review summarizes the contribution of research in plant physiology to the development of new technologies of peony production and flowering advancement. Despite the popularity of cut peonies, several factors still restrict their production: complicated flowering physiology, challenges in mass propagation, and postharvest handling. Further research of biochemical and molecular mechanisms, as well as breeding of new cultivars will promote the development of the peony industry and facilitate the creation of a Global Peony Chain for the successful marketing of this beautiful flower.

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Herbaceous peony tryptophan decarboxylase confers drought and salt stresses tolerance

Cited by 25 publications

References 43 publications

Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila

Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila

Germplasm resources and genetic breeding of Paeonia: a systematic review

Cut peony industry: the first 30 years of research and new horizons

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