HER2 biosensing through SPR-envelope tracking in plasmonic optical fiber gratings

Abstract

In the biomedical detection context, plasmonic tilted fiber Bragg gratings (TFBGs) have been demonstrated to be a very accurate and sensitive sensing tool, especially well-adapted for biochemical detection. In this work, we have developed an aptasensor following a triple strategy to improve the overall sensing performances and robustness. Single polarization fiber (SPF) is used as biosensor substrate while the demodulation is based on tracking a peculiar feature of the lower envelope of the cladding mode resonances spectrum. This method is highly sensitive and yields wavelength shifts several tens of times higher than the ones reported so far based on the tracking of individual modes of the spectrum. An amplification of the response is further performed through a sandwich assay by the use of specific antibodies. These improvements have been achieved on a biosensor developed for the detection of the HER2 (Human Epidermal Growth Factor Receptor-2) protein, a relevant breast cancer biomarker. These advanced developments can be very interesting for point-of-care biomedical measurements in a convenient practical way.

PfHRP2 detection using plasmonic optrodes: performance analysis

“…This method corresponds to the transposition of the commercially-available Kretschmann prism configuration to unclad OFs [13]. Specific structures can be implemented to excite SPR with OFs such as the use of Tilted Fiber Bragg Gratings (TFBGs) [14][15][16][17], Long Period Fiber Gratings (LPFGs) [18,19], U-bent fibers [20,21], and many other lab on fiber (LOF) architectures [22,23]. These probes have already proven their high potential for biomarkers detection in very different media, especially inside human tissues [24,25] and body fluids [26,27] among others [28,29].…”

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

“…Another promising SPR biosensor for POC applications is based on optical fibers made of silica or polymers [89,91,[94][95][96]. Light propagates inside optical fibers via total internal reflection and the evanescent field on the surface is used to excite the plasmons on the outer metallic coating.…”

Hybrid LPG-FBG Based High-Resolution Micro Bending Strain Sensor

“…Various previous studies have aimed to improve the maximum functionality of Bragg grating sensors and approach detection for multiple characteristics of the measurement range [51][52][53][54]. Examples of methods used by previous papers to increase the sensitivity of FBGs are the Bragg grating technique, which uses a fine structure through a taper technique [19,29], and the Bragg grating based on a grating configuration [55,56].…”