Heptadecanoic acid inhibits cell proliferation in PC‑9 non‑small‑cell lung cancer cells with acquired gefitinib resistance

Xu, Changzhi; Wu, Pengfei; Gao, Jiajia; Zhang, Lanlan; Ma, Tengfei; Ma, Bingbing; Yang, Shuai; Shao, Guojian; Yu, Yang; Huang, Xunduan; Yang, Xingyuan; Zhang, Buchang

doi:10.3892/or.2019.7130

Cited by 22 publications

(20 citation statements)

References 37 publications

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“…To date, pentadecanoic acid (C15:0) and heptadecanoic acid (C17:0) have been widely utilized as internal standards in gas chromatography experiments, dairy food intake bio-markers, and bio-markers for coronary heart diseases and type II diabetes mellitus [ 25 , 26 , 27 , 28 ]. Interestingly, a recent study demonstrates that heptadecanoic acid (C17:0) can exert anti-cancer effects in gefitinib-resistant non-small cell lung carcinoma (NSCLC) cells, emphasizing the efficacy of OCFAs in targeting human lung cancer cells [ 29 ]. Nevertheless, investigations assessing the anti-cancer effects of odd-chain fatty acids (C15:0 and C17:0) are extremely limited, and no studies have evaluated the in vitro anti-cancer effects of pentadecanoic acid (C15:0).…”

Section: Introductionmentioning

confidence: 99%

Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

Nguyen

Moon

et al. 2020

Nutrients

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Saturated fatty acids possess few health benefits compared to unsaturated fatty acids. However, increasing experimental evidence demonstrates the nutritionally beneficial role of odd-chain saturated fatty acids in human health. In this study, the anti-cancer effects of pentadecanoic acid were evaluated in human breast carcinoma MCF-7/stem-like cells (SC), a cell line with greater mobility, invasiveness, and cancer stem cell properties compared to the parental MCF-7 cells. Pentadecanoic acid exerted selective cytotoxic effects in MCF-7/SC compared to in the parental cells. Moreover, pentadecanoic acid reduced the stemness of MCF-7/SC and suppressed the migratory and invasive ability of MCF-7/SC as evidenced by the results of flow cytometry, a mammosphere formation assay, an aldehyde dehydrogenase activity assay, and Western blot experiments conducted to analyze the expression of cancer stem cell markers—CD44, β-catenin, MDR1, and MRP1—and epithelial–mesenchymal transition (EMT) markers—snail, slug, MMP9, and MMP2. In addition, pentadecanoic acid suppressed interleukin-6 (IL-6)-induced JAK2/STAT3 signaling, induced cell cycle arrest at the sub-G1 phase, and promoted caspase-dependent apoptosis in MCF-7/SC. These findings indicate that pentadecanoic acid can serve as a novel JAK2/STAT3 signaling inhibitor in breast cancer cells and suggest the beneficial effects of pentadecanoic acid-rich food intake during breast cancer treatments.

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Section: Introductionmentioning

confidence: 99%

Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

Nguyen

Moon

et al. 2020

Nutrients

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“…Another study found that some OCFAs, such as heptadecanoic acid and omega‐3 polyunsaturated fatty acids, inhibited the proliferation and migration of lung cancer cells by promoting apoptosis and inhibiting the phosphatidylinositol 3‐kinase (PI3K)/Akt signaling pathway [ 61 ]. Heptadecanoic acid was also shown to induce increased apoptosis in lung cancer cells and enhance the cytotoxic effects of gefitinib [ 62 ]. However, the oncogenic mechanism that leads to the production of OCFAs remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Spatial metabolomics for evaluating response to neoadjuvant therapy in non‐small cell lung cancer patients

Shen

Sun

Zens

et al. 2022

Cancer Communications

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Background The response to neoadjuvant chemotherapy (NAC) differs substantially among individual patients with non‐small cell lung cancer (NSCLC). Major pathological response (MPR) is a histomorphological read‐out used to assess treatment response and prognosis in patients NSCLC after NAC. Although spatial metabolomics is a promising tool for evaluating metabolic phenotypes, it has not yet been utilized to assess therapy responses in patients with NSCLC. We evaluated the potential application of spatial metabolomics in cancer tissues to assess the response to NAC, using a metabolic classifier that utilizes mass spectrometry imaging combined with machine learning. Methods Resected NSCLC tissue specimens obtained after NAC (n = 88) were subjected to high‐resolution mass spectrometry, and these data were used to develop an approach for assessing the response to NAC in patients with NSCLC. The specificities of the generated tumor cell and stroma classifiers were validated by applying this approach to a cohort of biologically matched chemotherapy‐naïve patients with NSCLC (n = 85). Results The developed tumor cell metabolic classifier stratified patients into different prognostic groups with 81.6% accuracy, whereas the stroma metabolic classifier displayed 78.4% accuracy. By contrast, the accuracies of MPR and TNM staging for stratification were 62.5% and 54.1%, respectively. The combination of metabolic and MPR classifiers showed slightly lower accuracy than either individual metabolic classifier. In multivariate analysis, metabolic classifiers were the only independent prognostic factors identified (tumor: P = 0.001, hazards ratio [HR] = 3.823, 95% confidence interval [CI] = 1.716–8.514; stroma: P = 0.049, HR = 2.180, 95% CI = 1.004–4.737), whereas MPR (P = 0.804; HR = 0.913; 95% CI = 0.445–1.874) and TNM staging (P = 0.078; HR = 1.223; 95% CI = 0.977–1.550) were not independent prognostic factors. Using Kaplan‐Meier survival analyses, both tumor and stroma metabolic classifiers were able to further stratify patients as NAC responders (P < 0.001) and non‐responders (P < 0.001). Conclusions Our findings indicate that the metabolic constitutions of both tumor cells and the stroma are valuable additions to the classical histomorphology‐based assessment of tumor response.

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“…In addition, some studies have shown that pentadecanoic acid can improve hyperinsulinemia, protect islet cells and improve the inflammatory state of diabetic mice [23]. Heptadecanoic acid is an effective drug against NSCLC cells in vitro [24].…”

Section: Discussionmentioning

confidence: 99%

Variability of Volatile Compounds in the Medicinal Plant Dendrobium officinale from Different Regions

Huang

Zhang

et al. 2020

Molecules

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Dendrobium officinale Kimura et Migo, a rare and traditional medicinal plant, contains many nutrients such as polysaccharides, alkaloids, amino acids and so on. Different growth environment and intraspecific hybridization of different germplasm resources lead to large differences in the yield, quality and medicinal value of D. officinale. Here, the volatile compounds of D. officinale from four producing regions (Zhejiang, Fujian, Yunnan and Jiangxi) were analyzed to provide a certain reference value for the selection of a specific medicinal component in D. officinale breeding. Fresh stems of D. officinale germplasm resources were collected, and the chemical constituents were determined by gas chromatography-mass spectrometry. A total of 101 volatile compounds were identified, of which esters and alcohols accounted for 23 and 22. Hexacosane is the highest relative content of all volatile components. The highest content of hexacosane was observed in YA1 from Yunnan was 34.41%, and the lowest (23.41%) in JA1 from Jiangxi. Moreover, 5-10 unique substances were determined in different regions. A total of 17 medicinal components were detected, and three unique medicinal components were detected only in YA1, revealing that YA1 can provide raw materials for the application of specific medicinal substances extraction. A total of four toxic components were detected, but no toxic components were detected in JA1 from Jiangxi, suggested that the germplasm resources from Jiangxi could be exploited efficiently for breeding superior D. officinale specimens. The results provide a theoretical basis for the collection, protection and utilization of D. officinale germplasm resources in different regions.

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Heptadecanoic acid inhibits cell proliferation in PC‑9 non‑small‑cell lung cancer cells with acquired gefitinib resistance

Cited by 22 publications

References 37 publications

Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

Pentadecanoic Acid, an Odd-Chain Fatty Acid, Suppresses the Stemness of MCF-7/SC Human Breast Cancer Stem-Like Cells through JAK2/STAT3 Signaling

Spatial metabolomics for evaluating response to neoadjuvant therapy in non‐small cell lung cancer patients

Variability of Volatile Compounds in the Medicinal Plant Dendrobium officinale from Different Regions

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