Hepcidin Determination in Dried Blood by Microfluidic LC–MS/MS: Comparison of DBS and Volumetric Absorptive Microsampling for Matrix Effect and Recovery

Houbart, Virginie; Cobraiville, Gaël; Servais, Anne-Catherine; Napp, Aurore; Merville, Marie‐Paule; Fillet, Marianne

doi:10.4155/bio.15.181

Cited by 26 publications

(14 citation statements)

References 22 publications

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“…Good extraction recoveries were observed for vincristine and tariquidar in both biological matrices, demonstrating that the unbuffered 60% ACN extraction solvent provided a reliable extraction procedure for both analytes. Formic acid was not included as part of the extraction solvent despite its use in other VAMS-related methods [11,15,16]. No distinct improvements were seen in extraction recovery with the inclusion of this acid, likely because of its presence in both mobile phase solvents, making the sample immediately compatible with the mass spectrometer upon injection of 1 μL of sample into the solvent channel.…”

Section: Discussionmentioning

confidence: 99%

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Rosser

Atkinson

Nath

et al. 2022

J of Separation Science

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A liquid chromatography‐tandem mass spectrometry method was developed and validated for the simultaneous quantification of vincristine and tariquidar in 10 μL of mouse whole blood using volumetric absorptive microsampling devices. Samples were extracted from the devices and quantified against calibrators prepared in a human blood plasma matrix. Separation of vincristine and tariquidar was achieved using a Shimpack XR ODS III C18 stationary phase and H2O and methanol mobile phase solvents containing 0.1% formic acid, running a gradient elution at a flow rate of 0.2 mL/min over 6.0 min. The method was linear up to 1200 ng/mL (R2 > 0.99 for both analytes), with calibrator accuracy within ± 15% of the nominal concentrations and analyte coefficient of variance <15% for both vincristine and tariquidar. Pharmacokinetic assessment of both analytes was successfully applied in mice as both single‐agent therapy and combination therapy over a 24‐h period, and a 2.3‐fold increase in vincristine drug exposure was observed in combination with tariquidar. This study validates the use of this approach for longitudinal analysis of drug exposure in animal studies.

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Section: Discussionmentioning

confidence: 99%

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Rosser

Atkinson

Nath

et al. 2022

J of Separation Science

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show abstract

“…VAMS devices are calibrated to contain specified volumes of blood and exhibits minimal sample volume variation for samples containing different levels of hematocrit and does not suffer from inhomogeneity (Spooner et al, ; Qu et al, ). These devices are extremely useful for TK and PK studies using animals, as it reduces sample volume and number of animals, which follows the three “R's” guidelines of reduce, refine, and replace for in situ sampling to preserve animal health and welfare (Houbart et al, ). By reducing the number of animals, the interindividual differences in PK profiles is reduced (Kita & Mano, ).…”

Section: Microsamplingmentioning

confidence: 99%

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

Frey

Damon

Badu‐Tawiah

2019

Mass Spectrometry Reviews

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Recent advancements in the sensitivity of chemical instrumentation have led to increased interest in the use of microsamples for translational and biomedical research. Paper substrates are by far the most widely used media for biofluid collection, and mass spectrometry is the preferred method of analysis of the resultant dried blood spot (DBS) samples. Although there have been a variety of review papers published on DBS, there has been no attempt to unify the century old DBS methodology with modern applications utilizing modified paper and paper‐based microfluidics for sampling, storage, processing, and analysis. This critical review will discuss how mass spectrometry has expanded the utility of paper substrates from sample collection and storage, to direct complex mixture analysis to on‐surface reaction monitoring.

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“…DBS techniques have also been successful in identification of proteins of diagnostic interest such as hemoglobin in Sickle-cell disease and other hemoglobinopathies, apolipoproteins and ceruloplasmin to diagnose Wilson's disease [120][121][122]. Other peptides and protein biomarkers discovered using DBS include hepcidin [123], peginesatide [124] and insulin-like growth factor-1 [125].…”

Section: Omic Applicationsmentioning

confidence: 99%

Challenges and opportunities in blood flow through porous substrate: A design and interface perspective of dried blood spot

Kumar

Agrawal

Chatterjee

2019

Journal of Pharmaceutical and Biomedical Analysis

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Highlights  Comparative analysis of current blood microsampling devices is reviewed.  Intrinsic and extrinsic factors affecting blood flow in porous materials are presented.  Current advances in the design and development of micro devices and materials for development of blood microsamplers are presented.  Major clinical and preclinical applications of dried blood spot created by a blood microsampler device are discussed.

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Hepcidin Determination in Dried Blood by Microfluidic LC–MS/MS: Comparison of DBS and Volumetric Absorptive Microsampling for Matrix Effect and Recovery

Abstract: The data suggest that the combination of VAMS and phospholipid removal plates provides low matrix effect and high sensitivity, and constitutes an easy and promising protocol for hepcidin analysis.

Cited by 26 publications

References 22 publications

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Quantification of vincristine and tariquidar by liquid chromatography‐tandem mass spectrometry in mouse whole blood using volumetric absorptive microsampling for pharmacokinetic applications

Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications

Challenges and opportunities in blood flow through porous substrate: A design and interface perspective of dried blood spot

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