Hepatotoxicity induced by gibberellic acid in adult rats and their progeny

Troudi, Afef; Samet, Amira Mahjoubi; Zeghal, Najiba

doi:10.1016/j.etp.2009.08.010

Cited by 49 publications

(45 citation statements)

References 27 publications

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“…This is in accordance with Troudi et al (2009) who observed that intrahepatic blood vessels, central and portal veins are congested and their lining epithelia are eroded. This is besides an inflammatory leucocytic infiltrations, which were more pronounced in the livers of treated mother rats than in their offspring when mother rats were given daily 200 ppm GA 3 in drinking water from the 14th day of pregnancy until day 14 after delivery.…”

Section: Discussionsupporting

confidence: 78%

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

2013

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The aim of the present study was to investigate the protective role of grape seed extract (containing proanthocyandin) against the adverse effects of plant growth regulators (GA 3 (gibberellic acid) and IAA (indoleacetic acid)). The present data showed that the administration of either GA 3 and IAA caused undesirable changes in both hepatic and testicular structure. This was evidenced by a disturbed hepatic strands, pyknotic nuclei, central vein with collapsed endothelium, dilatation in bile sinusoids, congested blood vessel, binucleatd hepatocytes, lymphocytic infiltration, vacuolation, giant hepatic cells, increased Kupffer cells and karyoryxis. Additionally, it was shown that degenerative changes in the testis, spermatogenic arrest, moderate tubular necrosis, Leydig cell degeneration and reduction in the number and size of the seminiferous tubules with some spermatogonia detached from the basement membrane. Concerning flow cytometric study of the liver a significant decrease in G0/1 % and a significant increase in S phase %, G2/ M %, P 53 % and apoptosis % (sub G1) were detected. However, in testis the data recorded a significant decrease in the percentage of mature sperm (percentage of haploid cells) and a significant increase in the percentage of spermatide, diploid cells, P 53 and of apoptotic cells. On the other hand, a distinct recovery of the mentioned hepatic and testicular histopathological and cytogenetic disorders was observed when proanthocyanidin was supplemented to rats administered either of the plant growth hormones (GA 3 and IAA).

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Section: Discussionsupporting

confidence: 78%

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

2013

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“…It was evidenced that, the reactive oxygen resulting from oxidative stress in the tissue causes a significant alteration in the cellular structure resulting in tissue injury and cell damage. This is in accordance with Troudi et al (2009) who observed that intrahepatic blood vessels, central and portal veins are congested and their lining epithelia are eroded. This is besides an inflammatory leucocytic infiltrations, which were more pronounced in the livers of rats which were given daily doses of GA 3 in the drinking water.…”

Section: Discussionsupporting

confidence: 78%

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Hassan

Al-Rawi

2012

Cytotechnology

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The present study aims to investigate the heptonephro-protective effect of grape seeds proanthocyanidin extract (GSPE) against the risks induced by gibberellic acid (GA3) in male rats. The results recorded that GA3 caused a significant increase in total lipids, total cholesterol, triglycerides and LDL-C levels in serum, concomitant with a significant decrease in serum HDL-C. A significant increase in serum AST, ALT, urea and creatinine, while, a significant decrease in total protein content in serum was observed in rats given GA3. Hepatic and renal lipid peroxidation product (MDA) was significantly increased, meanwhile, total antioxidant capacity (TAC), glutathione, and catalase levels were significantly decreased. In addition, there was a negative change in liver structure including dilatation in the central veins with degeneration of endothelium cells and cellular injury around the veins as well as in the kidney structure such as lesion in both glomeruli and tubules, detachment of the Malpighian corpuscles from the Bowman's capsule's epithelium, shrinkage in the glomerular capillary network. However, almost all of these adverse effects seemed to be ameliorated by oral administration of GSPE with GA3 to rats for 2 month indicating the protective effect of grape seeds GSPE on GA3 induced oxidative stress in rats.

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“…This could be explained either by an increase of the food intake of lactating rats, as demonstrated in our previous study [41], and/ or by an increase of mitotic division induced by this phytohormone [36]. Growth perturbations of pups could be explained by the direct action of GA 3 on foetuses and pups via its transfer through milk, as demonstrated by our results.…”

Section: Discussionmentioning

confidence: 64%

“…Pups were counted, weighed and each litter reduced to eight pups (four males and four females if possible) in order to maintain a similar lactation performance and to ensure maternal care [22]. GA 3 intake, ingested daily by lactating rats, was calculated after measuring drinking water consumption as reported by our previous paper [41]. So each lactating rat treated with GA 3 ingested 10.04 mg GA 3 /day.…”

Section: Animals and Treatmentmentioning

confidence: 99%

Oxidative stress induced by gibberellic acid on kidney tissue of female rats and their progeny: biochemical and histopathological studies

et al. 2011

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Gibberellic acid (GA(3)) is an endogenous plant growth regulator used worldwide in agriculture. The objective of this study was to investigate the effects of GA(3) on the kidney function of adult rats and their pups. Female Wistar rats were given daily 200 ppm GA(3) in drinking water from the 14th day of pregnancy until day 14 after delivery. GA(3) induced nephrotoxicity, as evidenced by a reduction in the 24-h urine volume and an increase in plasma creatinine, urea and uric acid levels. Nephrotoxicity was objectified by a significant increase of malondialdehyde level and a decrease of antioxidant enzyme activities like catalase, superoxide dismutase, glutathione peroxidase and glutathione content in kidneys of suckling pups and their mothers. Kidney histological studies confirmed biochemical parameters. We concluded that the exposure of rats to GA(3) induced oxidative stress and histopathological changes in kidneys of suckling rats and their mothers during late pregnancy and early postnatal periods.

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Hepatotoxicity induced by gibberellic acid in adult rats and their progeny

Cited by 49 publications

References 27 publications

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

Proanthocyanidin as a cytogenetic protective agent against adverse effects of plant growth regulators supplementation in rats

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Oxidative stress induced by gibberellic acid on kidney tissue of female rats and their progeny: biochemical and histopathological studies

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