Hepatoprotective effects of early pentoxifylline administration on hepatic injury induced by concanavalin A in rat

Abstract

Tumor necrosis factor alpha (TNF-α) plays an important role in the pathogensis of hepatitis C virus (HCV) infection induced liver injury. This study aimed to evaluate the effects of TNF-α inhibition with pentoxifylline (PTX) on concanavalin A (Con A)-induced hepatic injury in rats. The rats were distributed among 3 groups: (i) control group (1 mL saline·week(-1) by intravenous injection (i.v.)); (ii) Con A treatment group (20 mg Con A·(kg body mass)(-1)·week(-1), i.v.), and (iii) rats treated with Con A and with PTX (200 mg PTX·(kg body mass)(-1)·day(-1), per oral) group. Blood samples and livers were collected at the end of weeks 1, 2, 4, and 8 of Con A treatment. Portal pressure (PP) was measured at the end of week 8. The administration of PTX was found to confer significant protection against the injurious effects of Con A on the liver, by reducing serum levels of aspartate aminotransferase, alanine aminotransferase, hepatic TNF-α, and malondialdehyde. Histopathological examination revealed that treatment with PTX significantly suppressed early inflammation, reduced alpha smooth muscle actin, and the apoptosis of hepatocytes induced by Con A. Moreover, PTX significantly (P < 0.05) reduced PP, and quantitative analyses of the area of fibrosis induced by treatment with Con A showed a significant reduction at the end of week 8. We conclude that rats treated with PTX revealed a more or less normal hepatocyte architecture as well as marked improvement in fibrosis and PP.

Pristimerin as a Novel Hepatoprotective Agent Against Experimental Autoimmune Hepatitis

“…Additionally, Con A injection resulted in neutrophils infiltration as indicated by increased level and immuno-expression of MPO in the hepatic tissue. The deleterious effects of Con A on liver have been similarly shown in previous investigations (Feng et al, 2008 ; Mohamed et al, 2014 ; El-Agamy, 2016 ). Noteworthy, Pris pretreatment reversed all of these Con A-induced biochemical and histopathological alterations leading to marked improvement of the hepatic function which indicates potent hepatoprotective activity of Pris.…”

The effects of tadalafil and pentoxifylline on apoptosis and nitric oxide synthase in liver ischemia/reperfusion injury

“…PTX, a methylxanthine derivative and a nonspecific type‐4 PDE inhibitor, is clinically used in the treatment of lower extremity claudication. PTX has been reported to exert vasodilator effect in addition to significantly suppressing inflammation and to reduce hepatocyte apoptosis in experimental models [27]. Takhtfooladi et al.…”