Hepatoprotective effect of pentoxifylline against D-galactosamine-induced hepatotoxicity in rats

Taye, Ashraf; El‐Moselhy, Mohamed A.; Hassan, Magdy K.; Ibrahim, Hanaa; Mohammed, Aliaa F

doi:10.1016/s1665-2681(19)31751-x

Cited by 26 publications

(15 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7C ). Since D-GalN is hepatotoxic 53 54 and sensitizes mice to LPS 55 56 , we determined whether this carbohydrate modulator also enhanced BLP-induced organ damage. We found ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

Lakshmikanth

Jacob

Kudva

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2−/− mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.

show abstract

“…7C ). Since D-GalN is hepatotoxic 53 54 and sensitizes mice to LPS 55 56 , we determined whether this carbohydrate modulator also enhanced BLP-induced organ damage. We found ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

Lakshmikanth

Jacob

Kudva

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In a model of D-galactosamine-induced hepatotoxicity in rats, Taye et al (2009) proved that pretreatment with PTX prevented D-galactosamine-induced reduction of antioxidant enzyme activities, superoxide dismutase, and attenuated the elevated malonal- (1 week), (C) PTX-treated rats (1 week), (D) Con A-treated rats (2 weeks), (E) PTX-treated rats (2 weeks), (F) Con A-treated rats (4 weeks), (G) PTXtreated rats (4 weeks), (H) Con A-treated rats (8 weeks), (I) PTXtreated rats (8 weeks). Con A, concanavalin A; Con A treated, rats administered 20 mg Con A·(kg body mass) -1 , i.v.…”

Section: Fig 4 (A-i) Photomicrographs Of Liver Sections From (A) Comentioning

confidence: 99%

Hepatoprotective effects of early pentoxifylline administration on hepatic injury induced by concanavalin A in rat

Mohamed

Elmelegy

El-Aziz

et al. 2014

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

Tumor necrosis factor alpha (TNF-α) plays an important role in the pathogensis of hepatitis C virus (HCV) infection induced liver injury. This study aimed to evaluate the effects of TNF-α inhibition with pentoxifylline (PTX) on concanavalin A (Con A)-induced hepatic injury in rats. The rats were distributed among 3 groups: (i) control group (1 mL saline·week(-1) by intravenous injection (i.v.)); (ii) Con A treatment group (20 mg Con A·(kg body mass)(-1)·week(-1), i.v.), and (iii) rats treated with Con A and with PTX (200 mg PTX·(kg body mass)(-1)·day(-1), per oral) group. Blood samples and livers were collected at the end of weeks 1, 2, 4, and 8 of Con A treatment. Portal pressure (PP) was measured at the end of week 8. The administration of PTX was found to confer significant protection against the injurious effects of Con A on the liver, by reducing serum levels of aspartate aminotransferase, alanine aminotransferase, hepatic TNF-α, and malondialdehyde. Histopathological examination revealed that treatment with PTX significantly suppressed early inflammation, reduced alpha smooth muscle actin, and the apoptosis of hepatocytes induced by Con A. Moreover, PTX significantly (P < 0.05) reduced PP, and quantitative analyses of the area of fibrosis induced by treatment with Con A showed a significant reduction at the end of week 8. We conclude that rats treated with PTX revealed a more or less normal hepatocyte architecture as well as marked improvement in fibrosis and PP.

show abstract

“…By this reason, the finding that PTX/CG combination did not change the enzyme activities was expectable. In other experimental models— D ‐galactosamine‐induced hepatotoxicity20 and carbon tetrachloride‐induced liver toxicity21 however, a reduction of the enzyme antioxidants SOD and GPx is established; it is suggested that the pretreatment of animals with PTX (50 or 100 mg kg −1 for 7–21 days) might prevent the oxidative damage by increasing antioxidant enzyme levels.…”

Section: Resultsmentioning

confidence: 99%

“…A few data concerning the antioxidant capacity of PTX were found in the literature. The findings in the both experimental models— D ‐galactosamine‐induced hepatotoxicity20 and carbon tetrachloride‐induced liver toxicity21 suggest that the hepatoprotective role of PTX might be determined to its antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

In vivo effects of pentoxifylline on enzyme and non‐enzyme antioxidant levels in rat liver after carrageenan‐induced paw inflammation

Vircheva

Alexandrova

Georgieva

et al. 2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

The present study aimed to investigate the effects of pentoxifylline (PTX) on the carrageenan (CG)-induced paw oedema and on the endogenous levels of cell enzyme and non-enzyme antioxidants in rat liver, 4 and 24 h after CG injection. PTX (50 mg kg(-1) , i.p.), administered 30 min before CG, decreased the paw oedema, 2-4 h after CG administration. The drug protected CG-induced decrease of glutathione (non-enzyme antioxidant) and had no effect on CG-unchanged activities of superoxide dismutase, glutathione peroxidase (enzyme antioxidants) and glucose-6-phosphate dehydrogenase (enzyme, important for the activity of GSH-conjugated antioxidant enzymes). The drug showed a good antioxidant capacity in chemical systems, generating reactive oxygen species. The present results suggest that the antioxidant activity of PTX might contribute to its beneficial effects in liver injuries.

show abstract

Hepatoprotective effect of pentoxifylline against D-galactosamine-induced hepatotoxicity in rats

Cited by 26 publications

References 28 publications

Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

Hepatoprotective effects of early pentoxifylline administration on hepatic injury induced by concanavalin A in rat

In vivo effects of pentoxifylline on enzyme and non‐enzyme antioxidant levels in rat liver after carrageenan‐induced paw inflammation

Contact Info

Product

Resources

About