Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats

Abstract

Background It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino rats were divided into four groups: group I (control group), healthy rates; group II (acetaminophen group), rates received acetaminophen for induction of liver fibrosis; group III (treated group), liver fibrosis of rates treated with Moringa oleifera extract; and group IV (prophylactic group), rates treated with Moringa oleifera extract before and after induction of liver fibrosis. Serum liver function parameters were quantified using a spectrophotometer, while tumor necrosis factor α (TNF-α) and transformed growth factor beta (TGF- β) in liver tissue homogenate by means of enzyme-linked immunosorbent assay (ELISA), and expression of liver tissue TNF-α and TGF-genes was measured by real-time PCR after extraction and purification. Hepatic tissue was also evaluated under a microscope for histopathological changes. Results Our results showed a significant decrease in liver enzymes, TNF-α, and TGF-β in the treated and prophylactic groups compared to the acetaminophen group, and our biochemical data were consistent with the histopathological findings confirming the hepatoprotective effect of Moringa oleifera extract. Conclusions Biochemical parameters and histopathology results provide evidence that Moringa oleifera ethanolic extract has a great potential to prevent and improve liver damage due to its protective activity.

Structure Modification Converts the Hepatotoxic Tacrine into Novel Hepatoprotective Analogs

“…THQ derivatives, especially compounds 1b , 2a , and 2b , surprisingly lowered the levels of IL-6, thereby dampening the inflammatory response in the liver. These results suggest that the anti-inflammatory activity of THQ derivatives may have contributed to their hepatoprotective effect. ,,,,− Additionally, decreased ROS production by THQ culminates in the suppression of cytokine activation, thereby attenuating the expression of pro-inflammatory mediators such as TNF- α and IL-6 …”

“…This suggests that the hepatoprotective effect of the compounds under investigation may be partly due to their antifibrotic activity by lowering the TGF-β level. 37,40,51 The administration of CCl 4 was associated with an increase in the expression of α-SMA, 69 a distinct actin isoform found in vascular smooth muscle cells but lacking functionality in skeletal muscle fibrogenesis. 70 However, α-SMA is important for the mobility and contraction of myofibroblasts 71 during hepatic fibrogenesis, so they may reach the damaged liver tissue.…”

Anti-liver fibrosis of green materials Moringa oleifera seed oils from Madura Island against hepatocellular carcinoma development

“…Previous studies have extensively described the ability of M. oleifera leaf extract in suppressing the rate of hepatocyte injury since it contains antioxidants and phenolic compounds that reduce oxidative stress caused by carcinogenic substances (Sadek et al, 2017). M. oleifera tested on mice induced by acetaminophen (trigger of liver fibrosis) result in decreasing TNF-α and TGF-β levels (Aly et al, 2020). M. oleifera extract inhibited the increase in mRNA and protein levels of interleukin-6, tumor necrosis factor-alpha, inducible nitric oxide synthase, and cyclooxygenease-2 (Muangnoi et al, 2012).…”

Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis

“…24(S)-Methyl-cholest-5-en-3b-ol (1). 1 H NMR (400 MHz, CDCl 3 ), signals assignable to six methyls [d H 0.66 (3H, s), 0.75 (3H, d, J = 6.8 Hz), 0.77 (3H, d, J = 6.8 Hz), 0.83 (3H, d, J = 6.8 Hz), 0.90 (3H, d, J = 6.8 Hz), and 0.99 (3H, s)], one oxygenated methine [d H 3.50 (1H, m)] and one olenic proton [d H 5.33 (1H, brd, J = 5.3 Hz)] were observed (Fig. S1 †).…”