Hepatoprotective effect of leaves of Morinda tinctoria Roxb. against paracetamol induced liver damage in rats

Subramanian, Mohanraj; Sadasivam, Balakrishnan; Chinnaiyan, Santhosh Kumar; Sekar, Vinoth Kumar; Chandu, Atul N

doi:10.1016/j.dit.2013.06.008

Cited by 28 publications

(17 citation statements)

References 13 publications

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“…Elevated serum levels of ASAT and ALAT are indicative of cellular leakage and loss of functional integrity of the hepatic cell membranes implying hepatocellular damage [37]. In the present study, high serum levels of ASAT and ALAT were detected in the diabetic group in comparison with the normal control.…”

Section: Discussionsupporting

confidence: 49%

Antidiabetic Effect of Thymus Satureioides Aqueous Extract in Streptozotocin-Induced Diabetic Rats

Kabbaoui

Chda

Mejrhit

et al. 2016

Int J Pharm Pharm Sci

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Objective:The aerial parts of Thymus satureioides have been traditionally used to treat diabetes mellitus and its complications in Morocco. The aim of the present study was to investigate the antidiabetic and antihyperlipidemic effects of the aqueous extract of Thymus satureioides aerial parts in streptozotocin (STZ) induced diabetic rats. Methods:Experimental diabetes was induced in overnight fasted rats by intraperitoneal injection of streptozotocin (45 mg/kg). Diabetic rats were orally administered with aqueous extract of Thymus satureioides (500 mg/kg b.w.) for 28 d. Glibenclamide (2 mg/kg), a standard antidiabetic drug, was used as a positive control drug. Body weight and fasting blood glucose (FGB) were measured every week. Oral glucose tolerance, change in lipid parameters, urea, creatinine, aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) levels of diabetic rats were evaluated at the end of the treatment.Results: Administration of Thymus satureioides aqueous extract to diabetic rats for 28 d reduced their fasting blood glucose levels significantly compared to the diabetic control rats. The extract improved body weight and glucose tolerance in diabetic rats. The antihyperlipidemic assessment of the extract revealed a decrease in plasma total cholesterol, triglycerides, LDL levels and an increase in HDL level in the plasma of treated diabetic rats. Furthermore, the biochemical liver and kidney functional tests have shown that serum biomarkers of liver and renal dysfunction were significantly reduced in treated diabetic rats. Conclusion:The present findings suggest that Thymus satureioides extract has both antidiabetic and antihyperlipidemic effects in experimental diabetic rats which can be beneficial in the management of diabetes and its complications.

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Section: Discussionsupporting

confidence: 49%

Antidiabetic Effect of Thymus Satureioides Aqueous Extract in Streptozotocin-Induced Diabetic Rats

Kabbaoui

Chda

Mejrhit

et al. 2016

Int J Pharm Pharm Sci

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“…Nearly 20,000 deaths and 250,000 new cases have been reported every year [42] . The percentage of liver toxicity due to various exposures is much higher in developing countries like India (8–30%) compared to advanced countries (2–3%) [51] . Oxidative stress plays a major role in the development of liver diseases.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl 4 challenged rats

2015

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The aim of the present study was to isolate hepatoprotective component from Urtica dioica Linn. (whole plant) against CCl4-induced hepatotoxicity in-vitro (HepG2 cells) and in-vivo (rats) model. Antioxidant activity of hydro alcoholic extract and its fractions petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (NBF) and aqueous fraction (AF) were determined by DPPH and NO radicals scavenging assay. Fractions were subjected to in-vitro HepG2 cell line study. Further, the most potent fraction (EAF) was subjected to in-vivo hepatoprotective potential against CCl4 challenged rats. The in-vivo hepatoprotective active fraction was chromatographed on silica column to isolate the bioactive constituent(s). Structure elucidation was done by using various spectrophotometric techniques like UV, IR, 1H NMR, 13C NMR and MS spectroscopy. Ethyl acetate fraction (EAF) of hydro-alcoholic extract of U. dioica possessed the potent antioxidant activity viz. DPPH (IC50 78.99 ± 0.17 μg/ml) and NO (IC50101.39 ± 0.30 μg/ml). The in-vitro HepG2 cell line study showed that the EAF prevented the cell damage. The EAF significantly attenuated the increased liver enzymes activities in serum and oxidative parameters in tissue of CCl4-induced rats, suggesting hepatoprotective and anti-oxidant action respectively. Column chromatography of most potent antioxidant fraction (EAF) lead to the isolation of 4-hydroxy-3-methoxy cinnamic acid (ferulic acid) which is responsible for its hepatoprotective potential. Hence, the present study suggests that EAF of hydro-alcoholic extract has significant antioxidant and hepatoprotective potential on CCl4 induced hepatotoxicity in-vitro and in-vivo.

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“…Excess paracetamol is oxidized to N-acetyl-pbenzoquinone imine (NAPQI) which is a toxic and is done by the hepatic cytochrome p450 system (CYP450) (Yen et al, 2007). Detoxi cation is normally expelled from NAPQI by GSH.GSH depletion occurs when using high doses of paracetamol and consequently the toxic NAPQI accumulation that binds to cellular proteins via cysteinyl sulfhydryl groups and forms NAPQI-protein adducts (Subramanian et al, 2013). This case results in the formation of reactive oxygen species (ROS) like superoxide anion (O 2 -) , hydrogen peroxide (H 2 O 2 ), and a hydroxyl root (OH − ) that impacts the cell membrane and stimulates lipid peroxide and also leads to liver necrosis (Chen et al, 2009).…”

Section: Antimicrobial Activity Of Biosynthesized Cuonpsmentioning

confidence: 99%

Biosynthesis of Copper Oxide Nanoparticles by Marine Streptomyces MHM38 and their Preventive Efficacy Against Paracetamol-Inducing Hepatic Damage of Albino Rats

Hamed

Gazwi

Youssif

2020

Preprint

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Abstract Biosynthesis methods, employing microorganisms, have emerged as an eco-friendly, clean and viable alternative to chemical and physical methods. The present study reports the biosynthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of copper oxide nanoparticles, the influence of some parameters such as concentration of copper sulphate, reaction time, filtrate to substrate ratio and pH were studied. 5mM CuSO4 was optimal for NP production. Well-defined CuONP formation occurred after 60 min incubation when equal volume of filtrate (cell-free supernatant) to substrate (CuSO4 solution) was added. NPs remained stable in aqueous solution with increasing time at pH 8. CuONPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) and finally the nature of the nanoparticles was identified by elemental analysis (EDX). Uv-vis spectroscopy of CuONPs exhibited peak at 550 nm which corresponds to the Surface Plasmon Resonance of CuONPs. Most of the particles are spherical in shape and size ranges from 1.06 – 6.5 nm analyzed using Transmission Electron Microscope (TEM). Antimicrobial activity of CuONPs was performed by well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939), fungi (Rhizoctonia solani, Fusarium solani, Aspergillus niger) and yeast (Candida albicans ATCC 10237) .The highest antimicrobial activities recorded were against Candida albicans ATCC 10237, were as Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the lesser activity. The preventive efficacy of CuONPs was evaluated against the oxidative stress induced by paracetamol (PAC) in albino rats. The biochemical findings of CuONPs groups appeared a significant (p˂0.05) diminish in the levels of ALT, AST, ALP, LDH, total and direct bilirubin, Urea, and Creatinine as compared to paracetamol group. Non-enzymatic and enzymatic antioxidants of CuONPs groups were significantly elevated (p˂0.05) in SOD and GSH levels and significantly low NO and MAD levels compared to the paracetamol group. Also, the histopathological examination of the CuONPs groups assured that the impact of improving CuONPs against paracetamol-induced liver damage.

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Hepatoprotective effect of leaves of Morinda tinctoria Roxb. against paracetamol induced liver damage in rats

Cited by 28 publications

References 13 publications

Antidiabetic Effect of Thymus Satureioides Aqueous Extract in Streptozotocin-Induced Diabetic Rats

Antidiabetic Effect of Thymus Satureioides Aqueous Extract in Streptozotocin-Induced Diabetic Rats

Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl 4 challenged rats

Biosynthesis of Copper Oxide Nanoparticles by Marine Streptomyces MHM38 and their Preventive Efficacy Against Paracetamol-Inducing Hepatic Damage of Albino Rats

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