Hepatoprotective effect of ethanol extract fromBerchemia lineateagainst CCl₄-induced acute hepatotoxicity in mice

Abstract

Objective: The present study investigates the hepatoprotective effect of Berchemia lineate ethanol extract (BELE) on CCl 4 -induced acute liver damage in mice. Materials and methods: Effect of BELE administrated for 7 consecutive days was evaluated in mice by the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), albulin (ALB), globulin (GLB), and total protein (TP) levels, as well as liver superoxide dismutase (SOD) activity and malondialdehyde (MDA) level. Moreover, histopathological examinations were also taken. SOD (291.17, 310.32, and 325.67 U/mg prot) and reduction of MDA (7.27, 6.77, and 5.33 nmol/mg prot) levels. Histopathological examinations also confirmed that BELE can ameliorate CCl 4 -induced liver injuries, characterized by extensive hepatocellular degeneration/necrosis, inflammatory cell infiltration, congestion, and sinusoidal dilatation. Discussion and conclusion: The results indicated that BELE possessed remarkable protective effect against acute hepatotoxicity and oxidative injuries induced by CCl 4 , and that the hepatoprotective effects of BELE may be due to both the inhibition of lipid peroxidation and the increase of antioxidant activity.

Star Anise (Illicium verum) oil administration alleviates carbon tetrachloride-induced hepatotoxicity in rats

“…Also, CCl4 injected rats (group 3) showed an increase in ALP level. These result was agreed with Deb, (1998) and Li et al, (2015). Theses alterations may be attributed to the increase synthesis ALP due to the biliary tract involvement (Muriel et al, 1992).…”

Protective Effect of Gallotannin-Enriched Extract Isolated from Galla Rhois against CCl4-Induced Hepatotoxicity in ICR Mice

“…These findings were consistent with those of many previous studies that showed administration of several antioxidant mixtures significantly decreased the levels of serum marker enzymes relative to vehicle groups in CCl 4 treated animals. For example, the level of ALT, ALP, and AST was significantly decreased by methanol extract of Carissa opaca leaves (MCL) [ 43 ], aqueous leaf extract of Persea Americana (AEPA) [ 44 ], Berchemia lineate ethanol extract (BELE) [ 45 ], methanol extract of Melaleuca styphelioides (MSE) [ 46 ], and polyphenols-enriched extract from Huangshan Maofeng green tea (HMTP) [ 47 ]. However, MCL treatment induced a decrease in LDH level [ 43 ], which may have been caused by differences in the composition or concentration of bioactive compounds among studies.…”

Protective effect and mechanism of ginsenoside Rg1 on carbon tetrachloride-induced acute liver injury

“…CCl 4 injury mouse model. A CCl 4 -induced acute liver injury model of mice was established as described by Li et al (22). Male Kunming mice (20±2 g; n=60) were purchased from the Experimental Animal Center of Anhui Medical University [animal license no.…”