Hepatoprotective Effect of Citrus Sinensis Peel Extract Against Isoniazid and Rifampicin-induced Liver Injury in Wistar Rats

Abstract

Tuberculosis (TB) is one of the leading causes of death in developing countries. One of the problems in controlling TB disease is that most anti-tuberculosis drugs are hepatotoxic. Citrus sinensis peel extract is the rich source of secondary metabolites with high potential effectiveness as an antioxidant. In the present study, we evaluate the hepatoprotective effect of Citrus sinensis peel ethanolic extract (CSPEE) on isoniazid and rifampicin-induced liver injury in Wistar rats. Twenty five adult male Wistar rats were divided into 5 groups of 5 each : control, (INH+RIF) (50 mg/kg bw once a day for 14 days), (INH+RIF) + various dose of CSPEE (300, 450, 600 mg/kg bw). CSPEE was given orally once a day for 14 days followed by administration of INH + RIF suspension. The measurement of serum ALT and AST were carried out on the 15th day. Histopathologic examination of the liver was also performed. The Serum ALT and AST of the rats that induced with INH + RIF were increased significantly (P<0.001) compare to those of control groups, and the histopathologic slides showed steatosis, vacuolation and necrosis of hepatic cells. The serum ALT and AST in groups treated with CSPEE were not significantly different (p>0.05) with those of control groups. The serum ALT and AST and histopathological examination of the liver of the group that administered 600 mg/kg CSPEE were closest to normal rats. Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats.

Effects of Ethanol Extract from Sunkist (Citrus sinensis L. Osbeck) Peels on Rat’s Estrus Cycle

“…Moreover, this complex will influence the gene expression of the cell. Kuhnle et al reported that orange peels contented 36 µg/100g wet weight of phytoesterogen based on the LC-MS 13 and C-Labelled standard. 18 Moreover, Shalaby et al reported that the presence of flavanones, flavones, and flavonols in Citrus sinensis and Citrus aurantifolia leaf or peel showed a powerful estrogen-like activity.…”

“…The filtered remaceration and maceration were evaporated by rotary evaporator at a temperature of 70 o C and then followed by concentrated using the oven at 40 o C until they become thick extracts. 12,13 After that, the ethanol extract of Sunkist peels was suspended into the sodium carboxymethylcellulose to form oral suspension. The oral suspension used 0.5% Sodium Carboxymethyl cellulose as vehiculum.…”

Sunkist Peel Ethanol Extract Ameliorates Diabetic Nephropathy in Streptozotocin-Induced Diabetic Wistar Rats

“…After 3 days, it was filtered by filter paper, and then the residue was re-macerated with 600 ml of 96% ethanol for 3 days. The filtered remaceration and maceration were evaporated by rotary evaporator at a temperature of 70°C and then followed by concentrated using the oven at 40°C until they become thick extracts [12], [13].…”

Role of Sunkist Peels Extract Against the Level of Estradiol and Maturity of Follicle in the Female Wistar Rat

“…was re-macerated with 600 ml of 70% ethanol for 3 days. The filtered maceration and maceration were evaporated by rotary evaporator at a temperature of 70°C and then followed by concentrated using the oven at 40 o C until they become thick extracts [11,12].…”