Hepatoprotective and nephroprotective effects of sardinelle (Sardinella aurita) protein hydrolysate against ethanol-induced oxidative stress in rats

Abstract

Ethanol consumption-induced oxidative stress that is a major etiological factor has been proven to play important roles in organs' injury. In the present study, we investigated the protective effect of fish protein hydrolysate prepared from the heads and viscera of sardinelle (Sardinella aurita) (SPH) against the toxicity of ethanol on the liver and kidney of adult male rats. Animals were divided into four groups of six animals each: group C served as control, group Eth received 30 % ethanol solution at the dose of 3 g/kg body weight, group SPH received only 7.27 mg of SPH/kg body weight, and group Eth-SPH received ethanol and SPH simultaneously at the doses of 30 % and 7.27 mg/kg body weight, respectively. All groups were treated by gavage way for 15 days. Ethanol treatment decreased the defense enzymatic system including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), which increased after the co-administration of SPH. Malondialdehyde (MDA) and toxicity biomarker levels such as aspartate transaminase (AST) and alanine transaminase (ALT) and alcaline phosphatase (ALP) and gamma-glutamyl transaminase (GGT) activities were enhanced after chronic ethanol treatment and reduced by co-treatment with SPH. The histological examination of the liver and kidney confirmed biochemical changes in ethanol-treated rats and demonstrated the protective role of SPH.

Rutin ameliorates oxidative stress and preserves hepatic and renal functions following exposure to cadmium and ethanol

“… 2012 ; Kamoun et al. 2017 ). The alterations in liver, serum and kidney oxidative status due to Cd and/or Cd + EtOH exposure observed in the present study also corroborated previous findings (Sharma et al.…”

Protective role of luteolin against bisphenol A‐induced renal toxicity through suppressing oxidative stress, inflammation, and upregulating Nrf2/ARE/ HO‐1 pathway

“…Malondialdehyde (MDA), superoxide dismutase (SOD), HO‐1 contents of glutathione (GSH), and glutathione peroxidase (GPx) in rat's kidney were estimated utilizing commercial kits (Cayman Chemical Co., Ann Arbor, MI, USA) as per the manufacturer's instruction .…”

Nutritional characteristics of marine fish Sardinella zunasi Bleeker and immunostimulatory activities of its glycoprotein

“…The species of genus Sardinella are taken as a good source of bioactive components. Previous investigation of genus Sardinella species has exhibited that the chemical or nutritional composition is rich in proteins, lipids (especially polyunsaturated fatty acids), and minerals [10][11][12][13] and signicant biological properties such as antioxidant, 14,15 antibacterial, 16 anticancer, 17 hepatoprotective and nephroprotective, 18 hypolipidemic, antiobesity and cardioprotective effects. 19 However, there is still a lack of more data about the chemical or nutritional composition of genus Sardinella, and there is no report about the chemical or nutritional composition of S. zunasi Bleeker.…”