Hepatoprotective activity of white horehound (Marrubium vulgare) extract against cyclophosphamide toxicity in male rats

Abstract

The hepatoprotective activity of Marrubium vulgare against cyclophosphamide toxicity in Wistar rats was evaluated. Adult male rats were divided into 4 groups of six each: a control group, a group injected with cyclophosphamide (150 mg/kg) for 3 days, a group orally given a Marrubium vulgare aqueous extract (500 mg of dry leaves /kg/day) for 30 days then treated with cyclophosphamide, and a group receiving only Marrubium vulgare for 30 days. After 33 days of treatment, activities of alanine amino transferase (ALAT), aspartate amino transferase (ASAT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) were determined in serum. Moreover, lipid peroxidation level and superoxide dismutase (SOD) activities, catalase (CAT) and glutathione peroxidase (GPx) were measured in liver. Alterations of these hepatic biomarkers and increased lipid peroxidation confirmed cyclophosphamide-induced liver toxicity. Cyclophosphamide also decreased the enzymatic defense system against oxidative stress. However, when this drug was administered in rats given Marrubium vulgare extract, all the biological parameters underwent much less alteration. Administration of Marrubium vulgare extract was found to be beneficial by attenuating cyclophosphamide-induced liver damage. The protective effect of the plant is mainly attributed to its antioxidant properties and the existence of phenolic acids and flavonoids, as highlighted by HPLC-based analysis.

Hepatoprotective and antioxidant properties of the aqueous extract of Olea europaea leaves against Diclofenac-induced liver damages in mice

“…It has been determined that ROS plays an essential role in pathogenesis of DF-induced hepatic injuries and free radicals. Administration of olive leaves extract reduced the elevation of level plasma ASAT, ALAT, caused by DF due to preventing the decline of antioxidant defense system and direct free radical scavenging activity The protective effect of AEOL can be attributed to the presence of phenolic acids and flavonoids which are able to release a hydrogen proton from their hydroxyl-group trap free radicals and prevent DF induced liver damage (Ettaya et al 2016). Thus, HPLC analysis showed that AEOL is rich in phenolic acids (gallic acid, epicatechin, vanillic acid, coumaric acid and resveratrol) and flavonoids (rutin and apigenin).…”

Phytochemical Profiling and Evaluation of Marrubium sivasense Aytaç, Akgül & Ekici for Antioxidant Activity and Inhibition Effects on α-Amylase, Lipoxygenase, Xanthine Oxidase and Tyrosinase Enzymes

“…In the literature, there are a number of papers promising about phytochemical properties and biological activities of diverse Marrubium species (7,(11)(12)(13)(14)(15)(16)(17)(18)(19). However, there is no information about phytochemistry and biological potent of endemic species M. sivasense.…”

“…A previous phytochemical studies on Marrubium species resulted with polyphenols (7), essential oils (8), labdane diterpenoids and sterols (9,10). Biological activity investigations of Marrubium species encompasses antibacterial (11), antioxidant, antiproliferative (12), antinociceptive (13), antidiabetic (14), antihypertensive (15), hepatoprotective (16), antiinflammatory (17), cardioprotective (18) and cytotoxic/cytostatic (19) potentials. Some Marrubium species are traditionally used to treat various diseases, including asthma, pulmonary infections, inflammation and hypotension, as cholagogues and sedative agents, and for pain relief.…”

Cosmetic Functional Ingredients from Botanical Sources for Anti-Pollution Skincare Products

“…Its aerial parts are official in Hungarian Pharmacopoeia VII [89], and the European Medicine Agency (EMA) approved in 2013 the treatment of cough associated to cold, mild dyspeptic complaints and temporary loss of appetite as indication for aerial part of this plant [90]. M. vulgare is reported to possess several biological activities, among which the most interesting are antihepatotoxic [91], antihyperglicemic [92], antibacterial [93], anti-inflammatory [94] and antioxidant properties [95]. In particular antioxidant properties may justify the widespread of Marrubium vulgare extracts, often prepared with peculiar extraction techniques, as natural cosmetic ingredients, with claims including anti-pollution, antioxidant, protective for irritated and stressed skin, detoxifying, soothing.…”