Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells

Sharma, Surendra Kumar; Arogya, Sheela Meruga; Bhaskarmurthy, Deepak Hiraganahalli; Agarwal, Amit; Velusami, Chandrasekaran Chinampudur

doi:10.4103/0973-1296.84237

Cited by 23 publications

(11 citation statements)

References 17 publications

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“…[34] Number of medicinal plants has been reported with good hepatoprotective activity with potential antioxidative mechanism due to presence of flavonoids and phenolic compounds. [353637] The protective role of this plant against liver toxicity might be through antioxidative effect of flavonoids rich in the plant.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of Ficus religiosa leaves against isoniazid+rifampicin and paracetamol induced hepatotoxicity

Parameswari¹,

Chetty²,

Chandrasekhar³

2013

Phcog Res

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Background:The present study was designed to investigate the hepato protective effect of methanolic extract of Ficus religisoa L., Moraceae, on isoniazid-rifampicin and paracetamol induced hepatotoxicity in rats.Materials and Methods:Male Wistar albino rats were divided into six groups; group 1 served as a control received vehicle (Distilled water), group 2 served as a toxic control, received isoniazid-rifampicin (100 mg/ kg, i.p.) or paracetamol 200mg/kg, p.o in sterile water, groups 3, 4 and 5 received 100, 200 and 300mg/kg bw, p.o. methanolic extract of F. religisoa along with INH+RIF or paracetamol and group 6 received Liv 52 as reference standard. All the treatment protocols followed 21 days for INH+RIF model and seven days for paracetamol model, after treatment rats were sacrificed and blood was used for biochemical and liver was used for histological studies.Results:Administration of INH+RIF and paracetamol caused a significant elevation in the levels of liver marker enzymes (P < 0.05 and P < 0.01) and thiobarbituric acid reactive substances (P < 0.001) in experimental rats. Administration of methanolic extracts of F. religisoa significantly prevented isoniazid-rifampicin and paracetamol induced elevation in the levels of serum diagnostic liver marker enzymes and TBARS level in experimental groups of rats. Moreover, total protein and reduced glutathione levels were significantly (P < 0.001) increased in treatment group. The effect of extract was compared with a standard drug, Liv 52. The changes in biochemical parameters were supported by histological profile.Conclusion:The methanolic extract of F. religisoa protects against isoniazid- rifampicin and paracetamol induced oxidative liver injury in rats.

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Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of Ficus religiosa leaves against isoniazid+rifampicin and paracetamol induced hepatotoxicity

Parameswari¹,

Chetty²,

Chandrasekhar³

2013

Phcog Res

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“…An aqueous P. urinaria extract inhibits HBV replication and expression in a HBV transient transfection model in vitro (Wu et al, 2015 ). Sharma et al ( 2011 ) reported that a methanolic extract of P. urinaria whole plant protects the Hep G2 cell line against tert-butyl hydroxide (t-BH)-induced cytotoxicity. An ethanolic extract of P. urinaria whole plant attenuates the acetaminophen-induced hepatotoxicity and inhibition of the cytochrome P450 CYP2E1 enzyme in mice (Hau et al, 2009 ).…”

Section: Pharmacological Activities Of P Urinaria mentioning

confidence: 99%

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Geethangili

Ding

2018

Front. Pharmacol.

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The genus Phyllanthus (L.) is one of the most important groups of plants belonging to the Phyllantaceae family. Phyllanthus urinaria (L.) is an annual perennial herbal species found in tropical Asia, America, China, and the Indian Ocean islands. P. urinaria is used in folk medicine as a cure to treat jaundice, diabetes, malaria, and liver diseases. This review provides traditional knowledge, phytochemistry, and biological activities of P. urinaria. The literature reviewed for this article was obtained from the Web of Science, SciFinder, PubMed, ScienceDirect, and Google Scholar journal papers published prior to December 2017. Phytochemical investigations reveal that the plant is a rich source of lignans, tannins, flavonoids, phenolics, terpenoids, and other secondary metabolites. Pharmacological activities include anticancer, hepatoprotective, antidiabetic, antimicrobial, and cardioprotective effects. Thus, this present review summarizes the phytochemical constituents and their biological activities including biological studies on various crude extracts and fractions both in vitro and in vivo, and on clinical trial information about P. urinaria. This review compiles 93 naturally occurring compounds from P. urinaria along with their structures and pharmacological activities. The review is expected to stimulate further research on P. urinaria, and its pharmacological potential to yield novel therapeutic agents.

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“…It is the most important site of intermediary metabolism responsible for the detoxification and excretion of various substances like xenobiotics by altering and expelling toxins and wastes ( Chaudhari and Mahajan, 2016 ). Owing to continuous exposure to the toxicant via the portal blood circulation, the liver is highly susceptible to damage by xenobiotics, drugs, and hazardous substances ( Sharma et al, 2011 ). Due to handling the metabolism of various proteins, lipids, carbohydrates, secretion of bile, and storage of vitamins, it is considered as one of the most vital organs ( Bechmann et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effect of Steroidal Glycosides From Dioscorea villosa on Hydrogen Peroxide-Induced Hepatotoxicity in HepG2 Cells

et al. 2018

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Dioscorea villosa, commonly known as “Wild Yam” and native to North America, is well documented for its pharmacological properties due to the presence of steroidal glycosides. However, the hepatoprotective potential of these compounds has not been studied so far. The present investigation was aimed to study the hepatoprotective effect of the steroidal glycosides from D. villosa against H2O2, a known hepatotoxin, in human liver cell line (HepG2). Cytotoxicity assessment was carried out in cells exposed to various concentrations (10–50 μM) of compounds for 24 h using MTT assay and morphological changes. All tested compounds were known and among them, spirostans (zingiberensis saponin I, dioscin, deltonin and progenin III) were found to be cytotoxic whereas, furostans (huangjiangsu A, pseudoprotodioscin, methyl protobioside, protodioscin, and protodeltonin) were non-cytotoxic. Further, HepG2 cells were pretreated with biologically safe concentrations (10, 30, and 50 μM) of non-cytotoxic compounds and then cytotoxic (0.25 mM) concentration of H2O2. After 24 h, cell viability was assessed by MTT and NRU assays, while morphological changes were observed under the microscope. The results showed that treatment of HepG2 cells with compounds prior to H2O2 exposure effectively increased cell viability in a concentration-dependent manner. Furthermore, huangjiangsu A, pseudoprotodioscin, methyl protobioside, protodioscin, and protodeltonin at 50 μM increased GSH level and decreased intracellular ROS generation against H2O2-induced damages. The results from this study revealed that compounds isolated from D. villosa have hepatoprotective potential against H2O2-induced cytotoxicity and ROS generation and could be promising as potential therapeutic agents for liver diseases.

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Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells

Cited by 23 publications

References 17 publications

Hepatoprotective activity of Ficus religiosa leaves against isoniazid+rifampicin and paracetamol induced hepatotoxicity

Hepatoprotective activity of Ficus religiosa leaves against isoniazid+rifampicin and paracetamol induced hepatotoxicity

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Hepatoprotective Effect of Steroidal Glycosides From Dioscorea villosa on Hydrogen Peroxide-Induced Hepatotoxicity in HepG2 Cells

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