Hepatoprotective activity of the ethanolic extracts of Dipterocarpus turbinatus leaves in Paracetamol induced hepatotoxicity in rats

Abstract

Objective: Dipterocarpus turbinatus is a large woody plant widely grown in Northeastern state, Tripura. Ethanol extract of leaves was evaluated for its antioxidant and hepatoprotective activity. D. turbinatusMaterials and Methods: The preliminary phytochemical study was carried out to find out the presence of various phytoconstituents in different extracts obtained by cold maceration. Antioxidant activity was done by Nitric oxide scavenging and DPPH* scavenging. Paracetamol (3gm/kg) was used to induce hepatotoxicity in rats and 200mg/kg & 400mg/kg of ethanol extract of leaves of (EEDT) was used for the study, while silymarin (50mg/kg) was used as a standard. D. turbinatus Blood was collected by retro-orbital puncture and various biochemical parameters were determined by photometric analysis.: Ethanolic extract showed different bioactive constituents like flavonoid, phenolic, alkaloid, tannin. ResultsPlant extract was exhibited antioxidant properties. Tested extracts showed a significant reduction in the elevated level of various biochemical parameters like SGOT, SGPT, ALP, total bilirubin & increase the level of protein level with the dose-dependent manner. Histopathological studies of the liver of the tested animal showed there is an improvement in the architecture of the hepatic cell with plant extract treatments.The results suggest that ethanolic extract Conclusion: of leaves in different dose shows significant hepatoprotective activity against paracetamol-induced D.turbinatus hepatotoxicity in rats.