Hepatoprotection of Mentha aquatica L., Lavandula dentata L. and Leonurus cardiaca L.

Abstract

The phenolic composition of hydroethanolic extracts of Mentha aquatica L., Lavandula dentata L. and Leonurus cardiaca L., obtained from plants grown under organic cultivation, was determined and their hepatoprotective effects were investigated in vitro. L. cardiaca extract was rich in phenylethenoid glycosides, especially lavandolifolioside (254 ± 36 μg/mg), whereas rosmarinic acid and eriodictyol-O-rutinoside were the major phenolic compounds of L. dentata and M. aquatica extracts, accounting for 68 ± 7 μg/mg and 145 ± 22 μg/mg, respectively. These differential phenolic components presumably account for their dissimilar antioxidant properties. While L. cardiaca extract showed moderate biological effects, M. aquatica extract displayed high antioxidant activity in chemical models, and that of L. dentata was effective in counteracting potassium dichromate-induced ROS generation in human hepatocarcinoma cells. Moreover, M. aquatica extract (50 μg/mL) and its mixture (50%/50%) with L. dentata extract displayed an effective cytoprotective effect.

An analytical framework combining online high‐performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca

“…In the case of DPPH, the n-hexane, ethyl acetate, and dichloromethane extract did not show radical scavenging action, and there was a positive relation with the HPLC-online-DPPH of these extracts. Methanol extract exhibited a notably reduced DPPH scavenging activity, in agreement with the studies of Pereira et al, which found an EC50 value of 18.3 μg/mL in the DPPH test performed on L. cardiaca hydroethanolic extracts [37]. Furthermore, a study carried out by Jafari et al revealed that the hydroalcoholic extract of L. car-diaca had higher antioxidant activity (IC50 53.79 μg/mL) than ethyl acetate extract (IC50 107.16 ug/mL) [38].…”

“…Free radicals are accountable for oxidative damage to vital biomolecules. Keeping an equilibrium between oxidants and free radicals is a prerequisite for good health [8,37].…”

Micropropagation of lavender: a protocol for production of plantlets

“…Palavras-chave: Lavandula dentata, cultivo in vitro, óleos essenciais, multiplicação massal. demonstrated (Pereira et al, 2019). Martins et al (2019) identified more than 20 compounds in the essential oil of this species, which confirms its potential for the industry.…”

Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

“…To this end, the chemical compositions of tested L. cardiaca extracts were determined by HPLC-DAD-MS analysis. Data regarding L. cardiaca total bioactive compounds levels have been addressed in earlier studies [6,[26][27][28][29]. Different levels were observed in these studies.…”