Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability

Zeng, Jing; Wang, Jun; Cui, Yifu; Song, Xiaopeng; Zhang, Yizhe; Chen, Xuan; Hou, Ning; Teng, Yan; Lan, Yu; Chen, Ye-Guang; Yang, Xiao

doi:10.1093/cvr/cvaa016

Cited by 9 publications

(8 citation statements)

References 48 publications

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“… 46 , 65 , 66 HGF stimulates endothelial cell proliferation and migration through its specific receptor cellular-mesenchymal epithelial transition factor (c-Met) and promotes angiogenesis. 49 TGF-β1 has a dual effect on angiogenesis ( Fig. 1B ), when TGF-β1 binds to activator receptor like kinase 1 (ALK1) and induces recombinant mothers against decapentaplegic homolog 1/ recombinant mothers against decapentaplegic homolog 5(SMAD1/SMAD5) phosphorylation, resulting in angiogenesis.…”

Section: Resultsmentioning

confidence: 99%

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Platelet Rich Plasma in the Repair of Articular Cartilage Injury: A Narrative Review

Liang

Wang

et al. 2022

CARTILAGE

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Objective This paper reviews the research of platelet-rich plasma (PRP) in articular cartilage injury repair, to assess the mechanism, utilization, and efficacy of PRP in the treatment of articular cartilage injury, hoping to provide a theoretical basis for the clinical application of PRP in the future. Materials and Methods A comprehensive database search on PRP applications in cartilage repair was performed. Among them, the retrieval time range of PRP in clinical trials of repairing knee cartilage injury was from January 1, 2021 to January 1, 2022. Non-clinical trials and studies unrelated to cartilage injury were excluded. Result PRP can affect inflammation, angiogenesis, cartilage protection, and cellular proliferation and differentiation after articular cartilage injury through different pathways. In all, 13 clinical trials were included in the analysis. Conclusion PRP is an emergent therapeutic approach in tissue engineering. Most studies reported that PRP has a positive effect on cartilage injury, improving the joint function, meanwhile there is a lack of standardized standards. The technology of PRP in the repair and treatment of articular cartilage injury is worthy of further research.

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Section: Resultsmentioning

confidence: 99%

“…Yu et al 49 Connective tissue growth factor (CtgF) Promotes platelet adhesion, leukocyte migration and angiogenesis, and regulates collagen synthesis.…”

Section: Mechanisms Of Prpmentioning

confidence: 99%

Platelet Rich Plasma in the Repair of Articular Cartilage Injury: A Narrative Review

Liang

Wang

et al. 2022

CARTILAGE

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“…On the basis of the list of differentially abundant metabolites found in each comparison, there were 5 and 16 differential metabolites that were specifically identified in Phases D1 and D3D7 when compared with D0 (Figure 5a), and they were mainly involved in tryptophan metabolism and tyrosine metabolism (Supporting Information: Supplemental Figure 2). Studies demonstrated that tryptophan metabolism was potentially involved in the stress response (Le Floc'h et al, 2011) and tyrosine metabolism was associated with energy metabolism (Yu et al, 2021). An overview of the pathway analysis revealed that the DLS vascularization was accompanied by a series of changes in metabolism, which contributed to the different biological activities of the specific phase.…”

Section: Resultsmentioning

confidence: 99%

Dynamic alterations of metabolites revealed the vascularization progression of bioengineered liver

Zhou

Guo

Chen

et al. 2022

Biotech & Bioengineering

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Vascularization is a critical but challenging process in developing functional bioengineered livers with the decellularized liver scaffolds (DLSs) and the process is accompanied by cell-specific metabolic alterations. To elucidate the dynamic alterations of metabolites during vascularization, rat DLSs were vascularized with human umbilical vein endothelial cells and liquid chromatography mass spectrometrybased metabolomics was performed on culture supernatants collected at 0, 1, 3, 7, 14, and 21 days. Overall, 1698 peak pairs or metabolites were detected in the culture supernatants, with 309 metabolites being positively identified. The orthogonal partial least-squares discriminant analysis and functional enrichment analysis revealed three phases that could be clearly discriminated, including Phase D1 (cell proliferation and migration), Phase D3D7 (vascular lumen formation), and Phase D14D21 (functional endothelial barrier formation). Seventy-two common differentially abundant metabolites of known identity were detected in these three phases when compared with Day 0. Of these metabolites, a high level of β-Alanine indicated a better degree of vascularization and 14 days of in vitro dynamic culture is required to develop a functionalized vascular structure. These results enriched our understanding of the metabolic mechanism of DLS vascularization and indicated that β-Alanine could function as a potential predictor of the patency of vascularized bioengineered livers.

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“…HGS (hepatocyte growth factor-regulated tyrosine kinase substrate) is involved in tight junction protein trafficking and ECs permeability [ 39 ]. HGS is necessary for maintaining cerebrovascular stability [ 40 ]. PDGFB (platelet-derived growth factor subunit B) is expressed at a very low level in healthy vessels [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Epigenome-Wide Changes in the Cell Layers of the Vein Wall When Exposing the Venous Endothelium to Oscillatory Shear Stress

et al. 2023

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Epigenomic changes in the venous cells exerted by oscillatory shear stress towards the endothelium may result in consolidation of gene expression alterations upon vein wall remodeling during varicose transformation. We aimed to reveal such epigenome-wide methylation changes. Primary culture cells were obtained from non-varicose vein segments left after surgery of 3 patients by growing the cells in selective media after magnetic immunosorting. Endothelial cells were either exposed to oscillatory shear stress or left at the static condition. Then, other cell types were treated with preconditioned media from the adjacent layer’s cells. DNA isolated from the harvested cells was subjected to epigenome-wide study using Illumina microarrays followed by data analysis with GenomeStudio (Illumina), Excel (Microsoft), and Genome Enhancer (geneXplain) software packages. Differential (hypo-/hyper-) methylation was revealed for each cell layer’s DNA. The most targetable master regulators controlling the activity of certain transcription factors regulating the genes near the differentially methylated sites appeared to be the following: (1) HGS, PDGFB, and AR for endothelial cells; (2) HGS, CDH2, SPRY2, SMAD2, ZFYVE9, and P2RY1 for smooth muscle cells; and (3) WWOX, F8, IGF2R, NFKB1, RELA, SOCS1, and FXN for fibroblasts. Some of the identified master regulators may serve as promising druggable targets for treating varicose veins in the future.

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Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability

Cited by 9 publications

References 48 publications

Platelet Rich Plasma in the Repair of Articular Cartilage Injury: A Narrative Review

Platelet Rich Plasma in the Repair of Articular Cartilage Injury: A Narrative Review

Dynamic alterations of metabolites revealed the vascularization progression of bioengineered liver

Epigenome-Wide Changes in the Cell Layers of the Vein Wall When Exposing the Venous Endothelium to Oscillatory Shear Stress

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