Hepato Protective Effect of S-Adenosyl-L-Methionine Against Isoniazid Induced Hepatitis in Animal Model

Abstract

BACKGROUNDS-Adenosyl-L-Methionine (SAM) protects liver cells in viral hepatitis and paracetamol-induced hepatic damage, etc. But, there are scanty data regarding hepatoprotective effect of SAM in isoniazid-induced liver damage. With this background, the present study is undertaken to assess the activity of SAM in isoniazid-induced liver damage in animal model. MATERIALS AND METHODSHepatic injury was induced in albino rats by administering isoniazid (INH) at a dose of 54 mg/kg orally once daily for 30 days and served as the animal model of hepatitis. S-Adenosyl-L-Methionine (SAM) at dose of 48 mg/kg once daily was administered one hour prior to administration of INH to other group of rats (SAM pretreated group) for same 30 days duration and act as the test group. Silymarin 100 mg/kg was used as the standard hepatoprotective drug in the study. The liver enzymes and bilirubin level in serum were measured in above treatment groups and were compared statistically. Histopathology of liver was also compared among different groups. RESULTSThere was a significant lowering of liver enzymes in SAM pretreated group in comparison to the model group of INH-induced hepatitis. Histological examination also showed protective effect on liver architecture in rats pretreated with SAM followed by INH. CONCLUSIONThe results of the animal study clearly demonstrated the hepatoprotective effects of SAM against isoniazid-induced hepatitis.