Hepato nephropathology associated with inclusion body hepatitis complicated with citrinin mycotoxicosis in a broiler farm

Kumar, Manoj; Munusamy, Palanivelu; Latheef, Shyma K.; Singh, S. D.; Dhama, Kuldeep

doi:10.14202/vetworld.2018.112-117

Cited by 9 publications

(15 citation statements)

References 23 publications

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“…By the age of 1 month and even more so by 2 months, the control group birds undergo an increase in the liver weight, accompanied by cell hypertrophy prevailing over their proliferation that is consistent with the literature data [29,35]. This is proved by the nuclear-cytoplasmic ratio calculations.…”

Section: Microscopic Changes In the Liver On 30-60 Dayssupporting

confidence: 90%

“…Only half of the chickens had fat degeneration, lymphoid cell infiltration of the surrounding bile, and blood vessels connective tissue. The authors explain this by the subclinical infection caused by Clostridium perfringens and viruses, which lead to cholangiohepatitis as well as by aflatoxins, coming with the feed [29,[36][37][38].…”

Section: Microscopic Changes In the Liver On 15 Th Daysmentioning

confidence: 99%

“…The morphological study of the liver, using modern techniques (morphometry, histochemistry, and electron microscopy), allows to evaluate objectively the main parameters of metabolism, to reveal organ pathology, which develops as a result of the negative effect of crude protein high level in the diet, mycotoxin intake, and subclinical infections [3,6,19,[23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant influence on poultry liver morphology and hepatocyte ultrastructure

et al. 2019

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Background and Aim: The poultry farming development is held back by necessity to use the concentrates with the increased number of crude protein, mycotoxicoses, and subclinical infections concentration. They make a significant impact on the liver, therefore affecting its morphofunctional condition. Antioxidants use can prevent the negative influence of these factors. This study aimed to examine the impact of feed supplements containing natural antioxidants and synthetic antioxidants. Materials and Methods: The Muscovy ducks, Hungarian White geese, and quails were the study object. Birds after hatching from eggs were split into two groups: Control and two experimental. The control group (40 birds of each species) received a normal diet in accordance with the type and age. The young ducklings, goslings, and quails of the first experimental group (30 birds of each species) received water with diisopropylammonium dichloroacetate (Dironax). The young ducklings, goslings, and quails of the second experimental group (30 birds of each species) received liquid multivitamin preparation, containing organic selenium form (Solvimin Selen) from the 1st day of the postembryonic development to the age of 60 days. We performed the weighing of the young ducklings, goslings, and quails, determined the live weight, liver weight, using the electronic scales (measurement inaccuracy is 0.02 g). To conduct the morphometric, histological, and electron microscopic studies liver, we killed the birds at the age of 1 day, 15 days, a month and 2 months during the postembryonic ontogenesis. Results: The performed overall studies allowed to determine the positive influence of the antioxidants on growth and development of the meat bird, whose body mass increased by 5-10% in comparison with the control parameters. The antioxidants use prevents the development of fatty, hydropic and parenchymal degeneration, hepatocyte and epithelial cells necrosis of the bile ducts, and connective tissue proliferation with its further fibrosis. Conclusion: This study proved that it is more effective to use well-digestible, fast-acting natural polyvitaminic antioxidant complex with selenium, starting from the 1st day of the postembryonic ontogeny.

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Section: Microscopic Changes In the Liver On 30-60 Dayssupporting

confidence: 90%

Section: Microscopic Changes In the Liver On 15 Th Daysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant influence on poultry liver morphology and hepatocyte ultrastructure

et al. 2019

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“…Using Ziehl-Neelsen stain, acid-resistant bacteria were excluded. Differential diagnoses for granulomatous and necrotising hepatitis include toxic, ischemic or infectious causes [47,48]. In the presented investigations, only a limited number of samples could be investigated for pesticides.…”

Section: Plos Onementioning

confidence: 99%

Health status of free-ranging ring-necked pheasant chicks (Phasianus colchicus) in North-Western Germany

Liebing

Völker²,

Curland

et al. 2020

PLoS ONE

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Being a typical ground-breeding bird of the agricultural landscape in Germany, the pheasant has experienced a strong and persistent population decline with a hitherto unexplained cause. Contributing factors to the ongoing negative trend, such as the effects of pesticides, diseases, predation, increase in traffic and reduced fallow periods, are currently being controversially discussed. In the present study, 62 free-ranging pheasant chicks were caught within a two-year period in three federal states of Germany; Lower Saxony, North Rhine-Westphalia and Schleswig-Holstein. The pheasant chicks were divided into three age groups to detect differences in their development and physical constitution. In addition, pathomorphological, parasitological, virological, bacteriological and toxicological investigations were performed. The younger chicks were emaciated, while the older chicks were of moderate to good nutritional status. However, the latter age group was limited to a maximum of three chicks per hen, while the youngest age class comprised up to ten chicks. The majority of chicks suffered from dermatitis of the periocular and caudal region of the head (57-94%) of unknown origin. In addition, intestinal enteritis (100%), pneumonia (26%), hepatitis (24%), perineuritis (6%), tracheitis (24%), muscle degeneration (1%) and myositis (1%) were found. In 78% of the cases, various Mycoplasma spp. were isolated. Mycoplasma gallisepticum (MG) was not detected using an MG-specific PCR. Parasitic infections included Philopteridae (55%), Coccidia (48%), Heterakis/Ascaridia spp. (8%) and Syngamus trachea (13%). A total of 8% of the chicks were Avian metapneumovirus (AMPV) positive using RT-PCR, 16% positive for infectious bronchitis virus (IBV) using RT-PCR, and 2% positive for haemorrhagic enteritis virus (HEV) using PCR. All samples tested for avian encephalomyelitis virus (AEV), infectious bursal disease virus (IBDV) or infectious laryngotracheitis virus

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“…At present, citrinin detection methods include thin-layer chromatography [ 9 ], high-performance liquid chromatography [ 10 ], and liquid chromatography–tandem mass spectrometry (LC-MS) [ 11 ]. Thin-layer chromatography has the advantages of simple operation, low cost, and fast detection.…”

Section: Introductionmentioning

confidence: 99%

Research on the Mechanism of Action of a Citrinin and Anti-Citrinin Antibody Based on Mimotope X27

et al. 2020

Toxins

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Immunoassays are developed based on antigen–antibody interactions. A mimotope is an effective recognition receptor used to study the mechanism of action of antigens and antibodies, and is used for improving the sensitivity of the antibody. In this study, we built a 3D structure of the citrinin (CIT) mimotope X27 and anti-CIT single-chain antibody fragment (ScFv) through a “homologous modeling” strategy. Then, CIT and X27 were respectively docked to anti-CIT ScFv by using the “molecular docking” program. Finally, T28, F29, N30, R31, and Y32 were confirmed as the key binding sites in X27. Furthermore, the result of the phage-ELISA showed that the mutational phage lost the binding activity to the anti-CIT ScFv when the five amino acids were mutated to “alanine”, thereby proving the correctness of the molecular docking model. Lastly, a site-directed saturation strategy was adopted for the sites (T28, F29, N30, R31, and Y32). Eighteen different amino acids were introduced to each site on average. The activities of all mutants were identified by indirect competitive ELISA. The sensitivities of mutants T28F, T28I, F29I, F29V, N30T, and N30V were 1.83-, 1.37-, 1.70-, 2.96-, 1.31-, and 2.01-fold higher than that of the wild-type, respectively. In conclusion, the binding model between the CIT and antibody was elaborated for the first time based on the mimotope method, thereby presenting another strategy for improving the sensitivity of citrinin detection in immunoassays.

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Hepato nephropathology associated with inclusion body hepatitis complicated with citrinin mycotoxicosis in a broiler farm

Cited by 9 publications

References 23 publications

Antioxidant influence on poultry liver morphology and hepatocyte ultrastructure

Antioxidant influence on poultry liver morphology and hepatocyte ultrastructure

Health status of free-ranging ring-necked pheasant chicks (Phasianus colchicus) in North-Western Germany

Research on the Mechanism of Action of a Citrinin and Anti-Citrinin Antibody Based on Mimotope X27

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