Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR

Leblanc, Danielle; Poitras, Élyse; Gagné, Marie-Josée; Ward, Pierre; Houde, Alain

doi:10.1016/j.ijfoodmicro.2010.02.016

Cited by 64 publications

(54 citation statements)

References 38 publications

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“…The UK abattoir survey had found that one in five pigs had an active HEV infection as they entered the food chain [15]. This same finding of active HEV infections in slaughter-age pigs is found worldwide [32,33,34,35]. There was no evidence from the current study that PRRSV infection adversely affected the proportion of HEV infected pigs entering the food chain.…”

Section: Discussionsupporting

confidence: 74%

UK Pigs at the Time of Slaughter: Investigation into the Correlation of Infection with PRRSV and HEV

Frossard

Grierson

Cheney

et al. 2017

Viruses

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Hepatitis E virus (HEV) and porcine reproductive and respiratory syndrome virus (PRRSV) and are both globally prevalent in the pig population. While HEV does not cause clinical disease in pigs, its zoonotic potential has raised concerns in the food safety sector. PRRS has become endemic in the United Kingdom (UK) since its introduction in 1991, and continues to cause considerable economic losses to the swine industry. A better understanding of the current prevalence and diversity of PRRSV and HEV in the UK, and their potential association, is needed to assess risks and target control measures appropriately. This study used plasma, tonsil, and cecal content samples previously collected from pigs in 14 abattoirs in England and Northern Ireland to study the prevalence of several pathogens including PRRSV and HEV. The diversity of PRRSV strains detected in these samples was analyzed by sequencing open reading frame 5 (ORF5), revealing no substantial difference in PRRSV strains from these clinically unaffected pigs relative to those from clinical cases of disease in the UK. Despite the potential immuno-modulatory effect of PRRSV infection, previously demonstrated to affect Salmonella and HEV shedding profiles, no significant association was found between positive PRRSV status and positive HEV status.

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Section: Discussionsupporting

confidence: 74%

UK Pigs at the Time of Slaughter: Investigation into the Correlation of Infection with PRRSV and HEV

Frossard

Grierson

Cheney

et al. 2017

Viruses

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“…Thus, we provided molecular evidence of the presence of HEV in pigs in this country. Leblanc et al (2010) detected HEV RNA in feces and samples from several organs (lymph node 25.5%, bile 18.6%, feces 13.9%, and liver 20.9%) of pigs killed in slaughterhouses in Canada. In China, Li et al (2009) found that 3.5% of pig livers obtained from slaughterhouses tested positive for HEV RNA.…”

Section: Discussionmentioning

confidence: 98%

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Gutiérrez-Vergara¹,

Quintero²,

Duarte³

et al. 2015

Genet. Mol. Res.

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“…However, detection of the viral genome by PCR is technically challenging. Indeed, even in investigations on farm-raised animals (such as swine) where HEV infection is widespread, the threshold of HEV RNA detection is around 10 3 to 10 4 copies per ml of serum or per gram of faeces 28 29. Manipulation of blood samples, conditions of serum conservation for many years in some cases, and repeated freeze-thaw cycles of samples, are possible causes of HEV RNA degradation.…”

Section: Discussionmentioning

confidence: 99%

Chronic hepatitis E infection in children with liver transplantation

Halac

Béland²,

Lapierre³

et al. 2011

Gut

Self Cite

111

105

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The diagnosis of HEV infection is technically challenging and should be made simultaneously with RT-PCR methods, viral load quantification and serological markers. In immunosuppressed children who develop chronic hepatitis, the prevalence of HEV is high and could explain the chronic liver inflammation potentially leading to cirrhosis. Re-infection by different HEV strains from zoonotic transmission can result in progressive liver disease in immunocompromised children.

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Hepatitis E virus load in swine organs and tissues at slaughterhouse determined by real-time RT-PCR

Cited by 64 publications

References 38 publications

UK Pigs at the Time of Slaughter: Investigation into the Correlation of Infection with PRRSV and HEV

UK Pigs at the Time of Slaughter: Investigation into the Correlation of Infection with PRRSV and HEV

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Chronic hepatitis E infection in children with liver transplantation

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