Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Gutiérrez-Vergara, Cristian; Quintero, J.; Duarte, Joyce; Suescún, Jaime Parra; Herrera, Albeiro López

doi:10.4238/2015.march.31.20

Cited by 17 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the strains of HEV obtained from these patients were identified as genotype 3 [29,30]. Betancur et al reported a frequency of 11.25% for IgG anti-HEV in serum samples from pig farms workers in Antioquia [31], while Gutiérrez et al detected the HEV genome in 26% of swine feces [33] and in 41.3% and 25% pig livers from slaughterhouses and grocery stores, respectively [35]. Forero et al also identified the HEV genome in 26.9% of porcine stool samples from slaughterhouses of different subregions of Antioquia, which belonged to genotype 3 [34,70].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

First evidence of the Hepatitis E virus in environmental waters in Colombia

et al. 2017

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is one of the main causes of acute viral hepatitis of enteric transmission. HEV has been detected in environmental samples in several countries from Europe and Asia, constituting a risk factor for waterborne infection. In Colombia, HEV has been identified in samples obtained from patients as well as from swine, but no environmental studies have been carried out. To determine if HEV is present in environmental waters, samples from the main source of drinking water plant and of wastewater system of eight municipalities and two villages of Antioquia state (North West Colombia), were collected between December 2012 and April 2014. The HEV genome was detected by RT-PCR in 23.3% (7/30) of the samples from the main source of drinking water plants and in 16.7% (5/30) from sewage. Viral concentrates obtained from three positive sewage samples were used to inoculate HepG2 cell cultures that were followed for one month; however, the viral genome was not detected in any cell culture. This study demonstrates the circulation of HEV in both source of drinking water plants and wastewater in Antioquia state, Colombia. The presence of HEV in environmental waters could be a risk for waterborne transmission in this population. The findings of the present study, together with the evidence of HEV circulation in human and swine in Colombia, should be consider by public health authorities for the development of surveillance programs and the inclusion of HEV infection diagnosis in the guidelines of viral hepatitis in the country. This is the first report of HEV in environmental samples in Colombia and the second one in Latin America.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Besides, serological and molecular evidence of HEV infection was established in fecal and liver samples of swine [33–35]. …”

Section: Introductionmentioning

confidence: 99%

First evidence of the Hepatitis E virus in environmental waters in Colombia

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Very recent studies have reported high infection rates of HEV subtype 3a in pigs from Antioquia . Interestingly, HEV RNA had been previously detected in 25% of pig livers from grocery stores in the same geographical region …”

Section: Detection Of Hev In Domestic and Wild Animals In South Americamentioning

confidence: 99%

Hepatitis E virus in South America: The current scenario

Pisano

Martinez‐Wassaf²,

Mirazo

et al. 2018

Liver International

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is one of the most frequent causes of acute viral hepatitis of enteric transmission worldwide. In South America the overall epidemiology has been little studied, and the burden of the disease remains largely unknown. A research of all scientific articles about HEV circulation in South America until November 2017 was carried out. Human seroprevalences of HEV varied according to the studied population: blood donors presented prevalence rates ranging from 1.8% to 9.8%, while reports from HIV‐infected individuals, transplant recipients and patients on hemodialysis showed higher prevalence rates. Only 2 cases of chronic hepatitis in solid‐organ transplant patients from Argentina and Brazil have been described. Detection of HEV in the swine population is widely prevalent in the region. Anti‐HEV antibodies have also been recently documented in wild boars from Uruguay. Although scarce, studies focused on environmental and food HEV detection have shown viral presence in these kind of samples, highlighting possible transmission sources of HEV in the continent. HEV genotype 3 was the most frequently detected in the region, with HEV genotype 1 detected only in Venezuela and Uruguay. HEV is widely distributed throughout South America, producing sporadic cases of acute hepatitis, but as a possible agent of chronic hepatitis. Finding the virus in humans, animals, environmental samples and food, show that it can be transmitted through many sources, alerting local governments and health systems to improve diagnosis and for the implementation of preventive measures.

show abstract

“…HEV‐3 was found in bile swine (9.6%) and effluent samples from slaughterhouses of Brazil (dos Santos, de Paula, de Oliveira, Marchevsky, & Pinto, ). In Colombia, an infection rate of 41.3% was informed when analysing HEV presence in pig livers, and more recently, a rate of 16.6% was reported in Uruguay after performing the same analysis (Gutiérrez‐Vergara, Quintero, Duarte, Suescún, & López‐Herrera, ; Mirazo et al, ). Likewise, in Argentina it has been also demonstrated the circulation of HEV (Munné et al, , ).…”

Section: Introductionmentioning

confidence: 99%

Detection of HEV in naturally infected swine from central Argentina by an optimized HEV/MS2 duplex RT‐qPCR

Marziali

Acosta

Bolatti

et al. 2019

Zoonoses and Public Health

View full text Add to dashboard Cite

Hepatitis E virus (HEV) is currently considered as a global health concern due to the recognition of its zoonotic transmission to humans, mainly from swine, and its association with the development of severe cases of hepatitis in human risk populations. The lack of updated data on HEV state of infection in swineherds of Argentina, and the necessity of robust technologies for its detection in complex biological samples, positions HEV as an emerging issue in public health. Here, we have optimized a RT‐qPCR with internal control for a more precise and accurate HEV RNA detection in swine stool samples. We implemented this optimized molecular tool to analyse the current epidemiological scenario of HEV infection in swine from the core region of commercial activity of Argentina. A total of 135 stool samples were collected from 16 different farms and tested for HEV presence, resulting in 11 positive cases (8.1%). Phylogenetic analysis demonstrated that all of them correspond to HEV genotype 3 and that different subtypes circulate in the region. Moreover, two of the detected strains presented a high nucleotide similarity with a previously identified isolate from human sewage discharges, suggesting the zoonotic transmission of HEV to humans. Collectively, this work provides a better understanding of HEV epidemiology in Argentina while contributes to the improvement of HEV detection technologies.

show abstract

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Cited by 17 publications

References 31 publications

First evidence of the Hepatitis E virus in environmental waters in Colombia

First evidence of the Hepatitis E virus in environmental waters in Colombia

Hepatitis E virus in South America: The current scenario

Detection of HEV in naturally infected swine from central Argentina by an optimized HEV/MS2 duplex RT‐qPCR

Contact Info

Product

Resources

About