Hepatitis C Virus Stability: The Issue!

Cardoso, M.; Koerner, K.; Hinz, Wibke; Lenz, Cornelia; Schwandt, Andrea; Kubanek, B

doi:10.1046/j.1423-0410.1999.7620124.x

Cited by 15 publications

(10 citation statements)

References 6 publications

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“…Entrapment of HCV in the clots and the inhibitory effects of heparin on the PCR have also been evaluated [4,13]. These observations are not in accordance with recent reports of Miskowsky et al [14], Damen et al [13] and Da Silva-Cardoso et al [5,6]. Miskowsky et al could not ®nd any difference in the concentration of circulating HCV RNA between samples collected in plasma tubes (EDTA or ACD) or serotubes [14].…”

Section: Discussionmentioning

confidence: 90%

“…Moreover, we considered, as signi®cant, an HCV RNA titre variation of almost 0.5 log. This criteria is more restrictive than that adopted by other authors [5,6].…”

Section: Discussionmentioning

confidence: 91%

“…Damen et al con®rmed the stability of HCV RNA in clotted blood stored at +4°C for 72 h [13]. Da Silva-Cardoso et al reported that after dilution and long-term storage (21 days at +4°C), the presence of HCV RNA was still detectable by using a sensitive qualitative test [5]. In a further study, these authors suggest that the presence of blood cells and the type of sample (serum or plasma) have only a small effect on HCV RNA concentration after storage at +4°C for 168 h [6].…”

Section: Discussionmentioning

confidence: 99%

“…However, immediate whole blood separation into plasma and packed red cells and sophisticated storage conditions are not always practicable for the blood transfusion service with mobile and/or decentralized blood collection units. More recently, some authors reported that HCV RNA is relatively stable in EDTA-collected whole blood samples which are kept at +4°C [5,6]. At present there are no data concerning the stability of HCV RNA after repeated cycles of freezing and thawing.…”

Section: Introductionmentioning

confidence: 99%

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The stability of hepatitis C virus RNA after storage at +4 °C

Gessoni¹,

Barin²,

Frigato³

et al. 2000

Journal of Viral Hepatitis

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One of the major issues in nucleic acid testing is how to store blood samples to obtain reliable results. We therefore studied hepatitis C virus (HCV) RNA concentration in samples after storage at +4 degrees C or freezing and thawing. Six HCV RNA-positive, untreated subjects were studied. Blood samples were collected from these subjects in plasma preparation tubes. The HCV RNA concentration was analysed after storage at +4 degrees C for 168 h or after five freeze-thaw cycles. For HCV RNA quantification we used a qualitative and a quantitative commercial test. After 168 h of storage at +4 degrees C, the HCV RNA concentration was similar to that observed at time-point 0 (5.025 log vs 5.492 log). In one sample we observed a significant fall in HCV RNA concentration. After five freeze-thaw cycles, the HCV RNA concentration was lower than that observed at time-point 0 (4.454 log vs 5.492 log) and in four samples we observed a significant fall in HCV RNA concentration. Our data suggest that HCV RNA is stable in whole blood samples stored at +4 degrees C for 168 h. Based on our results, we conclude that the standard procedures for transport of blood samples (at room temperature for a maximum of 5 h) and storage schedules (+4 degrees C for a maximum of 48 h) can be maintained without compromising the quality of results.

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Section: Discussionmentioning

confidence: 90%

“…Moreover, we considered, as signi®cant, an HCV RNA titre variation of almost 0.5 log. This criteria is more restrictive than that adopted by other authors [5,6].…”

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The stability of hepatitis C virus RNA after storage at +4 °C

Gessoni¹,

Barin²,

Frigato³

et al. 2000

Journal of Viral Hepatitis

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“…34 Studies of the stability of HCV by blood bank services have shown that HCV is quite stable for at least seven days in ser um and plasma stored at 4°C. 35,36 Temperature also has an impact on HCV survival as determined by HCV RNA titers, with loss of viraemia after short (f ive days) and long-term storage at room temperature, but relative stability of frozen samples even after several freeze-thaw cycles. 37 Abe and Konomi assessed HCV RNA detection in serum spotted and dried on to f ilter paper held at room temperature for one, two, three and four weeks.…”

Section: Hepatitis Cmentioning

confidence: 99%

Blood‐borne viruses and their survival in the environment: is public concern about community needlestick exposures justified?

Thompson

Boughton

Dore

2003

Australian and New Zealand Journal of Public Health

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Stability of hepatitis C virus RNA in blood samples by TaqMan real‐time PCR

Şener

Yapar

Bedir

et al. 2010

Clinical Laboratory Analysis

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The storage conditions of blood samples for reliable results are very important in hepatitis C virus (HCV) RNA amplification tests used in routine HCV analyses. According to many studies, storage conditions could affect the RNA stability for HCV RNA detection. We have studied HCV RNA stability in blood samples stored at 4 degrees C. Nineteen blood samples containing different HCV RNA levels were stored at 4 degrees C and they were then analyzed by TaqMAN real-time PCR method. HCV RNA levels remained almost stable (100%) at least for five weeks at this storage condition. However, among them, the stability period was up to 11 weeks in two of the samples. As with these findings, there was a slightly significant correlation between the positivity time and the beginning HCV RNA levels (r=0.474, P=0.040). We conclude that, blood samples can be stored at 4 degrees C for five weeks without any significant difference in detected HCV RNA level by using TaqMan real-time PCR.

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Hepatitis C Virus Stability: The Issue!

Cited by 15 publications

References 6 publications

The stability of hepatitis C virus RNA after storage at +4 °C

The stability of hepatitis C virus RNA after storage at +4 °C

Blood‐borne viruses and their survival in the environment: is public concern about community needlestick exposures justified?

Stability of hepatitis C virus RNA in blood samples by TaqMan real‐time PCR

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