Stability of hepatitis C virus RNA in blood samples by TaqMan real‐time PCR

Şener, Kenan; Yapar, Mehmet; Bedir, Orhan; Gül, Cem; Çoşkun, Ömer; Kubar, Ayhan

doi:10.1002/jcla.20354

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Most of these studies were conducted with small patient numbers with the aim of verifying routine sampling conditions. As a consistent finding, the HCV RNA load was in most instances stable for 72 h at 4 ° C [8][9][10][11][12]. However, storage at other conditions (e.g.…”

Section: Discussionmentioning

confidence: 57%

“…Several studies have investigated the influence of storage on the quantifiable HCV viral load in patient blood samples [8,9]. Most of these studies were conducted with small patient numbers with the aim of verifying routine sampling conditions.…”

Section: Discussionmentioning

confidence: 99%

“…room temperature) or for longer time periods at 4 ° C resulted in more discrepant outcomes. In some studies HCV RNA seemed to be relatively stable at 4 ° C for several weeks [8,9,11], but other studies found significantly decreasing viral loads in some samples stored for more than 72 h [10,13]. As a confounding issue, in all of these studies only a small number (2-19) of individual samples were analyzed, and different material (serum or plasma), storage conditions, and also variation of diagnostic assaysmade an inter-study comparison even more difficult.…”

Section: Discussionmentioning

confidence: 99%

“…Although previous studies showed that samples could be stored at 4 ° C for several weeks without significant loss of HCV RNA [8,9], the suitability of the diagnostic test system has to be proven in the above described context.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days

Berger

Salla

Keppler

et al. 2016

Transfus Med Hemother

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Background: The HCV RNA testing of potential cornea donors frequently relies on blood samples stored pre mortem. The recommended storage time of maximum 72 h frequently excludes a significant fraction of donors. Methods: The influence of storage time of EDTA plasma samples at 4 °C on the viral load measured with the Roche HCV Quantitative Test vs. 2.0 was evaluated for 43 samples from HCV-positive individuals. Results: The mean reduction of the viral load after 4 °C storage for 6-8 days was 0.46 log₁₀ IU/ml (range +0.17 to -1.66 log₁₀ IU/ml). After 1-3 days a mean loss of 0.19 log₁₀ IU/ml (range +0.30 to -1.41 log₁₀ IU/ml) and after 3-5 days of 0.32 log₁₀ IU/ml (range +0.36 to -1.81 log₁₀ IU/ml) was observed. In 23.3% of samples, a viral load reduction ≥ 1 log₁₀ IU/ml (1.0-1.81 log₁₀ IU/ml) was found after prolonged storage (5-8 days). In none of the samples did the HCV load fall below the detection limit. Conclusion: Plasma storage for up to 8 days can quantitatively reduce the HCV RNA load, yet has no influence on the reliability of a qualitative HCV RNA detection by this ultrasensitive test to determine the HCV status of serologically negative cornea donors.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days

Berger

Salla

Keppler

et al. 2016

Transfus Med Hemother

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“…Patients with non-responder HCV infection had monthly HCV RNA real time PCR tests. The assay and quantification were performed as described before (14).…”

Section: Follow-upmentioning

confidence: 99%

Efficacy of autologous mesenchymal stem cell transplantation in patients with liver cirrhosis

Kantarcıoğlu

Demirci²,

Avcu³

et al. 2015

Turk J Gastroenterol

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Background/Aims: Because of several limitations and complications of liver transplantation, new alternative treatment modalities are required for patients with liver cirrhosis. Many study results encourage the use of autologous bone marrow-derived mesenchymal stem cells for liver diseases. In this study, we assessed the impact of autologous mesenchymal stem cell transplantation on liver tissue and liver chemistry. Materials and Methods: Twenty-five patients with biopsy-proven liver cirrhosis were enrolled in the study. Patients received 1×10 6 autologous mesenchymal stem cells/kg via a peripheral vein. Biochemical parameters were checked monthly. Periodical radiological screening and liver biopsies before mesenchymal stem cell transplantation were performed after 6 months. Liver specimens were assessed by a pathologist. Results: No side effect was observed and the mesenchymal stem cell transplantation procedure was well tolerated. Twelve patients completed the study. In 8 patients, improvements in Model for End-Stage Liver Disease (MELD) scores were observed. Serum albumin levels markedly increased in the third month. In patients with nonresponder hepatitis C, HCV RNA levels both became negative after mesenchymal stem cell transplantation. Histopathological examinations of liver tissues before and at 6 months after transplantation revealed no change in liver tissue regeneration or fibrosis. However, in 5 patients, hepatitis activity index scores decreased. Conclusion: Autologous mesenchymal stem cell transplantation via peripheral vein is safe and feasible. Consecutive liver biopsy examinations suggested that mesenchymal stem cells could not reach the liver in a sufficient amount. Improvement in patients and clearance of HCV RNA may have occurred through immunomodulatory mediators secreted by transplanted mesenchymal stem cells, namely the "endocrine" effect.

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The acceptability of serum sample storage for hepatitis C viral load analysis in chronic hepatitis C patients

Huang

Liu

et al. 2019

Adv in Digestive Medicine

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The detectable time duration of stored serum sample containing hepatitis C virus (HCV) remains to be elucidated. This study aimed to elucidate the viral load changes of the stored serum samples under 4°C conditions. We measured the viral loads collected from 13 patients (8 males, age = 60.5 ± 17.5 years). The samples were kept under 4°C conditions before analysis. Besides baseline viral load, the samples then were measured at days 3, 5, 7, 9, and 11 after collection, respectively. The measured viral loads at different time points were compared with baseline samples by using the Abbott RealTime HCV Assay in an m2000 PCR System. The viral loads of the 13 patients ranged between 0.151 and 10 648.491 KIU/mL at baseline. There were no significant differences in viral load changes between the baseline and the samples of other time points. The differences were within 0.45 log10 IU/mL (P > 0.05) at different time points. The study demonstrated that serum samples kept at 4°C for 11 days were acceptable for molecular analysis.

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Stability of hepatitis C virus RNA in blood samples by TaqMan real‐time PCR

Cited by 8 publications

References 23 publications

HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days

HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days

Efficacy of autologous mesenchymal stem cell transplantation in patients with liver cirrhosis

The acceptability of serum sample storage for hepatitis C viral load analysis in chronic hepatitis C patients

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