Hepatitis C Virus Replicates in the Same Immune Cell Subsets in Chronic Hepatitis C and Occult Infection

Pham, Tram N. Q.; King, Dawn; MacParland, Sonya A.; McGrath, Jerry; Reddy, S. Bharati; Bursey, Ford; Michalak, Tomasz I.

doi:10.1053/j.gastro.2007.12.011

Cited by 127 publications

(174 citation statements)

References 32 publications

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“…In patients with CHC, HCV-RNA was detected in all various cell subtypes, and monocytes had the greatest viral load, but in OCI, B cells having higher HCV quantities compared to monocytes (Pham et al, 2008).…”

Section: Potential Pathogenic Mechanisms Resulting In An Ocimentioning

confidence: 96%

Occult Hepatitis C Infection and its Clinical Relevance in Lymphoproliferative Disorders

Agha¹,

Mashad²,

Mofreh³

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Potential Pathogenic Mechanisms Resulting In An Ocimentioning

confidence: 96%

Occult Hepatitis C Infection and its Clinical Relevance in Lymphoproliferative Disorders

Agha¹,

Mashad²,

Mofreh³

2017

Int.J.Curr.Microbiol.App.Sci

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“…Our study is the first report evaluating if/how the expression of miRNA-155 and miRNA-196b is related to the HCV RNA replication in PBMCs from chronic hepatitis C patients. Previous studies have confirmed that HCV RNA can replicate in PMBCs from chronically infected patients (3,4). As the replication of the genomic HCV RNA strand involves the synthesis of the antigenomic strand (the replication intermediate) we used the quantitative strand-specific RT-PCR to distinguish PBMCs, depending on the presence of the antigenomic strand.…”

Section: Discussionmentioning

confidence: 97%

“…However, several studies reported that both genomic and antigenomic HCV RNA strands were detected in PBMCs from chronically infected [chronic hepatitis C (CHC)] patients (3,4), giving the evidence that an in vivo virus can replicate in extrahepatic sites, like PBMCs.…”

mentioning

confidence: 99%

Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells

Sidorkiewicz¹

2011

Int J Mol Med

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Abstract.A tight relationship has been revealed between cellular microRNAs (miRNAs) and the course of hepatitis C virus (HCV) replication in human hepatoma cells. Although the detection of the antigenomic HCV RNA strand in peripheral blood mononuclear cells (PBMCs) has provided evidence for viral replication in PBMCs, no reports have shown how miRNAs are affected upon HCV RNA synthesis in PBMCs. The aim of the present study was to assess if and how the expression levels of miRNA-155 and miRNA-196b in PBMCs are related to HCV replication in PBMCs of chronic hepatitis C (CHC) patients. Supporting analyses were performed to evaluate the expression of precursor pri-miR-155 (BIC) and Dicer protein.The genomic and antigenomic HCV RNA strands in PBMCs were detected by strand-specific qRT-PCR. The expression levels of miRNAs, BIC RNA and Dicer protein were assayed on PBMCs by qRT-PCR and Western blotting, respectively. miRNA-155 and miRNA-196b were detected in all studied PBMC samples, but their levels varied according to the presence of the antigenomic HCV RNA strand in PBMCs. Increased expression levels of miRNA-155 and miRNA-196b were associated with the presence of the antigenomic HCV RNA strand in PBMCs. In this group of patients higher frequency of BIC RNA and Dicer protein detection was also found. This study demonstrates that HCV RNA replication in PBMCs of CHC patients is connected with the increased and coordinated expression of miRNA-155 and miRNA-196b.

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“…Anti-HCV pozitifken HCV RNA'nın negatif olması durumunda bazı olasılıklar değerlendirilmelidir. Bunlar, HCV'nin kan dolaşımı dışında PKMNH veya karaciğer gibi başka bir yerde sekestre olması, vireminin intermitan vasıfta olması dolayı-sıyla test esnasında HCV RNA'nın plazmada bulunmaması, HCV RNA'nın test için belirlenmiş olan sınır değerinin altında olması, virusla infekte olduktan sonra plazmada viral RNA kaybolmasına rağmen anti-HCV varlığının uzun süre devam etmesi veya anti-HCV'nin pasif olarak kan transfüzyonu sı-rasında kazanılabilmesidir (22)(23)(24)(25). Bu konuyla ilgili yapılan bir araştırmada HCV ile infekte diyaliz hastalarının %35'inde vireminin dalgalı bir seyir gösterdiği tespit edilmiştir (26).…”

Section: İrdelemeunclassified

Frequency and Epidemiologic Characteristics of Hepatitis C Virus Infection in Patients Receiving Hemodialysis in Our Region

Bozkurt¹,

Aygen²,

Yıldız³

et al. 2012

Klimik Dergisi

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ÖzetAmaç: Hepatit C virusu (HCV) hemodiyalize giren hasta popülas-yonunda sık görülen bir infeksiyon etkenidir. Çalışmada bölge-mizdeki hemodiyaliz hastalarında HCV infeksiyonunun sıklığının ve epidemiyolojik özelliklerinin araştırılması amaçlanmıştır. Yöntemler: Çalışmaya rutin olarak hemodiyalize giren 16 yaşın üzerindeki kronik böbrek yetmezliği olan 100 hasta alındı. Hastaların demografik verileri, HCV için olası bulaşma yolları, hemodiyalize giriş yolu, hemodiyaliz süresi, karaciğer fonksiyon testleri, hepatit göstergeleri ve HCV RNA sonuçları araştırıldı. Hepatit göstergeleri "enzyme immunoassay" (EIA, Abbott Architect, Almanya) ile test edildi. Plazmada HCV RNA "real time" polimeraz zincir reaksiyonu (PZR, COBAS TaqMan Roche, Almanya) ile araştırıldı. PZR testinin ölçüm aralığı 25-3.91x10 8 İÜ/ ml, duyarlılığı ise 25 İÜ/ml idi. Bulgular: Hastaların yaş ortalaması 58.6±13.9 yıldı ve %56'sı erkekti. Ortalama diyaliz süresi 4.8±3.9 (1-19) yıl bulundu. Alanin aminotransferaz ve aspartat aminotransferaz değerleri ortalaması sırasıyla 17±1.1 İÜ/lt ve 16.1±7.2 İÜ/lt idi. En sık bulaşma yolu cerrahi girişim, ikinci sıklıkla kan transfüzyonu olarak saptandı. Çalışmaya dahil edilen 100 hastanın 16'sında anti-HCV ve HCV RNA testlerinden en az biri pozitifti. Hastalardan 10'unda anti-HCV pozitifliği, 11'inde HCV RNA pozitifliği belirlendi. Anti-HCV'si pozitif olan 10 hastadan 5'inin HCV RNA'sı pozitif bulundu. Anti-HCV'si negatif olan 90 hastanın 6 (%6.7)'sında HCV RNA pozitifti. Bu hastaların HCV RNA değerleri 75-338 İÜ/lt arasındaydı. AbstractObjective: Hepatitis C virus (HCV) is a common cause of infection in patients receiving hemodialysis. In this study, we aimed to determine frequency and epidemiological characteristics of HCV infection in hemodialysis patients. Methods: One hundred patients with end-stage renal disease, over 16 years old, receiving hemodialysis were enrolled in this study. Demographic data, possible routes of transmission, type of vascular access, duration of hemodialysis, liver function tests, markers of hepatitis and HCV RNA were all evaluated. Serological markers were tested with enzyme immunoassay (EIA, Abbott Architect, Germany). HCV RNA was studied by using real-time polymerase chain reaction (PCR, COBAS TaqMan Roche, Germany). Measurement range of the PCR test was 25-3.91x10 8 IU/mL and the sensitivity of the test was 25 IU/mL. Results: The mean age of the patients was 58.5±13.9 years and 56% were male. The average duration of dialysis was 4.8±3.9 (1-19) years. The mean levels of aspartate aminotransferase and alanine aminotransferase were 17±1.1 IU/L and 16.1±7.2 IU/L, respectively. Surgical procedures and blood transfusion were the most common route of transmission. Of the 100 patients, 16 were positive for at least one of anti-HCV and HCV RNA. 10 out of 100 patients were positive for anti-HCV and 11 were positive for plasma HCV RNA. Five of the 10 patients whose anti-HCV results were positive were also found to be positive for HCV RNA. Six (6.7%) out of 90 anti-HCV-negative patien...

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Hepatitis C Virus Replicates in the Same Immune Cell Subsets in Chronic Hepatitis C and Occult Infection

Cited by 127 publications

References 32 publications

Occult Hepatitis C Infection and its Clinical Relevance in Lymphoproliferative Disorders

Occult Hepatitis C Infection and its Clinical Relevance in Lymphoproliferative Disorders

Coordinated increase of miRNA-155 and miRNA-196b expression correlates with the detection of the antigenomic strand of hepatitis C virus in peripheral blood mononuclear cells

Frequency and Epidemiologic Characteristics of Hepatitis C Virus Infection in Patients Receiving Hemodialysis in Our Region

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